Homology and non-homology at the yeast mating type locus

Nature ◽  
1981 ◽  
Vol 289 (5795) ◽  
pp. 250-252 ◽  
Author(s):  
George F. Sprague ◽  
Jasper Rine ◽  
Ira Herskowitz
Keyword(s):  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus ◽  
Yeast Mating

scholarly journals Investigation of Mating Pheromone–Pheromone Receptor Specificity in Lentinula edodes

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 506
Author(s):  
Sinil Kim ◽  
Byeongsuk Ha ◽  
Minseek Kim ◽  
Hyeon-Su Ro
Keyword(s):  
Mating Type ◽  
Lentinula Edodes ◽  
Signal Pathway ◽  
Recombinant Yeast ◽  
Mating Pheromone ◽  
Type Locus ◽  
Pheromone Receptor ◽  
Mating Type Locus ◽  
Yeast Mating ◽  
Mating Signal

The B mating-type locus of Lentinula edodes, a representative edible mushroom, is highly complex because of allelic variations in the mating pheromone receptors (RCBs) and the mating pheromones (PHBs) in both the Bα and Bβ subloci. The complexity of the B mating-type locus, five Bα subloci with five alleles of RCB1 and nine PHBs and three Bβ subloci with 3 alleles of RCB2 and five PHBs, has led us to investigate the specificity of the PHB–RCB interaction because the interaction plays a key role in non-self-recognition. In this study, the specificities of PHBs to RCB1-2 and RCB1-4 from the Bα sublocus and RCB2-1 from the Bb sublocus were investigated using recombinant yeast strains generated by replacing STE2, an endogenous yeast mating pheromone receptor, with the L. edodes RCBs. Fourteen synthetic PHBs with C-terminal carboxymethylation but without farnesylation were added to the recombinant yeast cells and the PHB–RCB interaction was monitored by the expression of the FUS1 gene—a downstream gene of the yeast mating signal pathway. RCB1-2 (Bα2) was activated by PHB1 (4.3-fold) and PHB2 (2.1-fold) from the Bα1 sublocus and RCB1-4 (Bα4) was activated by PHB5 (3.0-fold) and PHB6 (2.7-fold) from the Bα2 sublocus and PHB13 (3.0-fold) from the Bα5 sublocus. In particular, PHB3 from Bβ2 and PHB9 from Bβ3 showed strong activation of RCB2-1 of the Bβ1 sublocus by 59-fold. The RCB–PHB interactions were confirmed in the monokaryotic S1–10 strain of L. edodes by showing increased expression of clp1, a downstream gene of the mating signal pathway and the occurrence of clamp connections after the treatment of PHBs. These results indicate that a single PHB can interact with a non-self RCB in a sublocus-specific manner for the activation of the mating pheromone signal pathways in L. edodes.

scholarly journals Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus

Methods ◽  
2009 ◽  
Vol 48 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Toyoko Tsukuda ◽  
Kelly M. Trujillo ◽  
Emmanuelle Martini ◽  
Mary Ann Osley
Keyword(s):  
Chromatin Remodeling ◽  
Mating Type ◽  
Strand Break ◽  
Double Strand Break ◽  
Type Locus ◽  
Mating Type Locus ◽  
Yeast Mating ◽  
Dna Double Strand Break

scholarly journals Identification of a protein that binds to the Ho endonuclease recognition sequence at the yeast mating type locus.

1997 ◽  
Vol 17 (2) ◽  
pp. 770-777 ◽  
Author(s):  
R Wang ◽  
Y Jin ◽  
D Norris
Keyword(s):  
Mating Type ◽  
Recognition Sequence ◽  
Mobility Shift ◽  
Vitro Assay ◽  
Type Locus ◽  
Mating Type Locus ◽  
Yeast Mating ◽  
Mating Type Switching

Mating type switching in Saccharomyces cerevisiae initiates when Ho endonuclease makes a site-specific double-stranded break at MAT, the yeast mating type locus. To identify other proteins involved in this process, we examined whether extracts prepared from ho- mutants contain additional factors that bind near the recognition sequence for Ho. Using an electrophoretic mobility shift assay, we isolated a chromatographic fraction that contains an activity, named YZbp, which binds to two sequences flanking the recognition sequence at MATalpha and to one sequence overlapping it at MATa. MAT plasmids carrying mutations in the YZbp recognition sequence are cleaved by purified Ho at wild-type efficiencies in an in vitro assay. These same plasmids, however, are not cleaved by Ho inside cells, demonstrating that YZbp acts as a positive activator of in vivo cleavage. YZbp is present in all cell types, even those not undergoing mating type switching, suggesting that it has additional cellular functions.

scholarly journals Deletions and single base pair changes in the yeast mating type locus that prevent homothallic mating type conversions.

1983 ◽  
Vol 80 (11) ◽  
pp. 3401-3405 ◽  
Author(s):  
B. Weiffenbach ◽  
D. T. Rogers ◽  
J. E. Haber ◽  
M. Zoller ◽  
D. W. Russell ◽  
...  
Keyword(s):  
Mating Type ◽  
Base Pair ◽  
Type Locus ◽  
Single Base ◽  
Mating Type Locus ◽  
Yeast Mating ◽  
Single Base Pair

scholarly journals rme1 Mutation of Saccharomyces cerevisiae: map position and bypass of mating type locus control of sporulation.

1981 ◽  
Vol 1 (10) ◽  
pp. 958-960 ◽  
Author(s):  
J Rine ◽  
G F Sprague ◽  
I Herskowitz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus ◽  
Type Information

Sporulation in Saccharomyces cerevisiae normally occurs only in MATa/MAT alpha diploids. We show that mutations in RME1 bypassed the requirements for both a and alpha mating type information in sporulation and therefore allowed MATa/MATa and MAT alpha/MAT alpha diploids to sporulate. RME1 was located on chromosome VII, between LEU1 and ADE6.

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