Vesicle formation and acetylene reduction activity in Frankia sp. CPI1 cultured in defined nutrient media

Nature ◽  
1980 ◽  
Vol 287 (5783) ◽  
pp. 633-635 ◽  
Author(s):  
John D. Tjepkema ◽  
William Ormerod ◽  
John G. Torrey
Keyword(s):  
Acetylene Reduction ◽  
Acetylene Reduction Activity ◽  
Vesicle Formation ◽  
Nutrient Media ◽  
Reduction Activity

Effect of O2 on vesicle formation, acetylene reduction, and O2-uptake kinetics in Frankia sp. HFPCcI3 isolated from Casuarina cunninghamiana

1985 ◽  
Vol 31 (9) ◽  
pp. 804-809 ◽  
Author(s):  
Marcia A. Murry ◽  
Zhang Zhongze ◽  
John G. Torrey
Keyword(s):  
Diffusion Barrier ◽  
Acetylene Reduction ◽  
Root Nodules ◽  
Acetylene Reduction Activity ◽  
Vesicle Formation ◽  
Reduction Activity ◽  
Casuarina Cunninghamiana ◽  
Protection Mechanisms ◽  
Large Phase

The effect of the partial pressure of oxygen (PO2) on the formation of vesicles, which are thought to be the site of N2 fixation in Frankia, was studied in HFPCcI3, an effective isolate from Casuarina cunninghamiana. Unlike other actinorhizal root nodules, vesicles are not produced by the endophyte in Casuarina nodules. However, in culture under aerobic conditions, large, phase-bright vesicles are formed in HFPCcI3 within 20 h following removal of [Formula: see text] from the culture medium and reach peak numbers within 72 to 96 h. In vivo acetylene reduction activity parallels vesicle formation. Optimum rates of acetylene reduction in short-term assays occurred at 20% O2 (0.2 atm (1 atm = 101.325 kPa)) in the gas phase. O2 uptake (respiration) determined polarographically showed diffusion-limited kinetics and remained unsaturated by O2 until 300 μM O2. In contrast, respiration in [Formula: see text]-grown cells was saturated by O2 between 8 and 10 μM O2. These results indicate the presence of a diffusion barrier associated with the vesicles. Vesicle development was repressed in cells incubated in N-free media sparged with gas mixtures with PO2 between 0.001 and 0.003 atm. Nitrogenase was induced under these conditions, but acetylene reduction was extremely O2 sensitive. The kinetics of O2 uptake as a function of dissolved O2 concentration in avesicular cells were similar to those in [Formula: see text]-grown cells indicating the lack of a diffusion barrier. These results demonstrate that vesicle formation and the development of the O2 protection mechanisms of nitrogenase are regulated by ambient PO2 in HFPCcI3.

Nitrogen fixation associated with 'Park' Kentucky bluegrass (Poapratensis L.)

1979 ◽  
Vol 25 (10) ◽  
pp. 1197-1200 ◽  
Author(s):  
R. C. Shearman ◽  
W. L. Pedersen ◽  
R. V. Klucas ◽  
E. J. Kinbacher
Keyword(s):  
Nitrogen Fixation ◽  
Acetylene Reduction ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Acetylene Reduction Activity ◽  
Controlled Environment ◽  
Nitrogen Accumulation ◽  
Reduction Activity ◽  
Significant Difference ◽  
Reduction Assay

Associative nitrogen fixation in Kentucky bluegrass (Poa pratensis L.) turfs inoculated with five nitrogen-fixing bacterial isolates was evaluated using the acetylene reduction assay and nitrogen accumulation as indicators of fixation. 'Park' and 'Nugget' Kentucky bluegrass turfs were grown in controlled environment chambers and inoculated with Klebsiella pneumoniae (W-2, W-6, and W-14), Erwinia herbicola (W-8), and Enterobacter cloacae (W-11). 'Park' inoculated with K. pneumoniae (W-6) had significant acetylene reduction activity using undisturbed turfs. Other treatments including turfs treated with heat-killed cells had no significant difference in acetylene reduction. In a second study, 'Park' and 'South Dakota Certified' turfs were grown in a greenhouse and inoculated with K. pneumoniae (W-6) and E. herbicola (W-8). 'Park' inoculated with K. pneumoniae (W-6) had increased acetylene reduction activity rates and also a greater nitrogen accumulation in aerial tissues when compared to controls. Acetylene reduction activity was correlated (r = 0.92) to nitrogen accumulation. Other treatments did not effectively increase acetylene reduction activity or nitrogen accumulation.

scholarly journals Diversity and Effectivity of Indigenous Mesorhizobium Strains for Chickpea (Cicer arietinum L.) in Myanmar

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 287 ◽  
Author(s):  
Khin Myat Soe ◽  
Aung Zaw Htwe ◽  
Kyi Moe ◽  
Abiko Tomomi ◽  
Takeo Yamakawa
Keyword(s):  
Sequence Analysis ◽  
Cicer Arietinum ◽  
Acetylene Reduction ◽  
Dry Weight ◽  
Acetylene Reduction Activity ◽  
Rrna Gene ◽  
Cicer Arietinum L ◽  
Reduction Activity ◽  
Legume Production ◽  
High Level

Chickpea (Cicer arietinum L.) is one of the world’s main leguminous crops that provide chief source of food for humans. In the present study, we characterized thirty isolates of indigenous chickpea rhizobia from Myanmar based on the sequence analysis of the bacterial 16S rRNA gene. The sequence analysis confirmed that all isolates were categorized and identified as the genus Mesorhizobium and they were conspecific with M. plurifarium, M. muliense, M. tianshanense, and M. sp. This is the first report describing M. muliense, M. tianshanense, and M. plurifurium from different geographical distribution of indigenous mesorhizobia of chickpea in Myanmar. In order to substitute the use of chemical fertilizers in legume production, there is a need for the production of Biofertilizers with rhizobial inoculants. The effectiveness of Myanmar Mesorhizobim strains isolated from soil samples of major chickpea growing areas of Myanmar for plant growth and nitrogen fixation were studied in pot experiments. The nodule dry weight and acetylene reduction activity of the plant inoculated with Mesorhizobium tianshanense SalCP19 was significantly higher than the other tested isolates in Yezin-4 chickpea variety. But, Mesorhizobium sp. SalCP17 was showed high level of acetylene reduction activity per plant in Yezin-6 chickpea variety.

Acetylene reduction activity in salt‐tolerant alfalfa andrhizobium

1989 ◽  
Vol 3 (4) ◽  
pp. 469-476 ◽  
Author(s):  
Ramzi M. Mohammad ◽  
W.F. Campbell ◽  
M.D. Rumbaugh
Keyword(s):  
Acetylene Reduction ◽  
Acetylene Reduction Activity ◽  
Salt Tolerant ◽  
Reduction Activity
Sign in / Sign up

Export Citation Format

Share Document