Genetic alteration of nerve membrane excitability in temperature-sensitive paralytic mutants of Drosophila melanogaster

Nature ◽  
1980 ◽  
Vol 286 (5775) ◽  
pp. 814-816 ◽  
Author(s):  
Chun-Fang Wu ◽  
Barry Ganetzky
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Alteration ◽  
Temperature Sensitive ◽  
Nerve Membrane ◽  
Membrane Excitability

scholarly journals INDIRECT SUPPRESSION INVOLVING BEHAVIORAL MUTANTS WITH ALTERED NERVE EXCITABILITY IN DROSOPHILA MELANOGASTER

Genetics ◽  
1982 ◽  
Vol 100 (4) ◽  
pp. 597-614 ◽  
Author(s):  
Barry Ganetzky ◽  
Chun-Fang Wu
Keyword(s):  
Drosophila Melanogaster ◽  
Potential Temperature ◽  
Temperature Sensitive ◽  
Membrane Excitability ◽  
Nerve Activity ◽  
Functional Relationships ◽  
Nerve Excitability ◽  
Excitable Membranes ◽  
Allele Specific ◽  
And Function

ABSTRACT Two classes of X-linked behavioral mutants of Drosophila melanogaster, leg-shaking mutants and bang-sensitive mutants, are suppressed by napts (no action potential, temperature-sensitive), an autosomal temperature-sensitive paralytic mutation. So far, napts is found to suppress thirteen mutations at seven loci, two of which produce leg shaking and five bang-sensitivity. Suppression is recessive, occurs at temperatures permissive for napts, and is indirect and function-specific rather than allele-specific. At restrictive temperatures, napts is known to completely block all nerve activity. Several of the mutants suppressed by napts are shown by neurophysiological experiments to have increased nerve excitability. The physiological defect of these mutants as well as their behavioral defect is suppressed by napts. Thus, suppression occurs within individual neurons at the level of excitable membranes and apparently depends on the reduction in membrane excitability caused by napts even under permissive conditions. We suggest that all mutants suppressed by napts may have related defects leading to enhanced nerve excitability. Genetic interactions of this type help reveal functional relationships between different behavioral mutants and suggest ways of isolating new mutants with altered excitable membranes.

scholarly journals THE MECHANISM OF A BROODING EFFECT ASSOCIATED WITH SEGREGATION DISTORTION IN DROSOPHILA MELANOGASTER

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 619-631
Author(s):  
D L Hartl
Keyword(s):  
Drosophila Melanogaster ◽  
Segregation Distortion ◽  
Mature Sperm ◽  
Temperature Sensitive ◽  
Short Time

ABSTRACT The recovery of the SD chromosome from a heterozygous SD male increases with brood. This is independent of the age of the female, occurs during the time the sperm are stored in the females, disappears when the segregation distortion is suppressed, and is temperature-sensitive-temperature shocks above or below 25°C applied to the mature sperm both tend to accelerate the increase in the recovery of SD. All this suggests the existence of a class of sperm affected by SD in which the sperm are able to fertilize eggs for a short time following ejaculation but become dysfunctional thereafter.

Genetic analysis of vitellogenesis in Drosophila melanogaster: the identification of a temperature-sensitive mutation affecting one of the yolk proteins

Development ◽  
1978 ◽  
Vol 47 (1) ◽  
pp. 111-120
Author(s):  
M. Bownes ◽  
B. D. Hames
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Temperature Sensitive ◽  
Large Reduction ◽  
Yolk Proteins ◽  
Protein Uptake ◽  
Temperature Sensitive Mutation ◽  
Transplant Experiments

A number of female sterile mutations on the first and third chromosomes of Drosophila melanogaster have been screened for defects in the yolk proteins using polyacrylamide gel electrophoresis. Two new mutants were identified. 6m45 accumulates all three yolk proteins (YP1, YP2 and YP3) in the haemolymph but they are all absent from the ovaries suggesting it is a yolk-protein-uptake mutant. In contrast, 1163 is a temperature-sensitive mutation with a large reduction in the quantity of YP1 in the haemolymph and ovaries at 29 °C. Both mutants are autonomous in ovary transplant experiments.

Studies on the female-sterile phenotype of 1(1)su(f) ts76a, a temperature-sensitive allele of the suppressor of forked mutation in Drosophila melanogaster

Development ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 247-256
Author(s):  
Thomas G. Wilson
Keyword(s):  
Cell Death ◽  
Drosophila Melanogaster ◽  
Follicle Cell ◽  
Lethal Mutation ◽  
Female Sterility ◽  
Temperature Sensitive ◽  
Egg Chambers ◽  
A Cell ◽  
Sensitive Allele

A new allele of the suppressor of forked [su(f)] mutation in Drosophila melanogaster has been found and designated 1(1)su(f)ts76a. It is temperature-sensitive for suppression of forked (f) and has additional temperature-sensitive phenotypes of lethality, female sterility, and abnormal bristle formation at 29 °C. It closely resembles two other conditional alleles of su(f), 1(1)su(f)ts67g and 1(1)ts726. Female sterility at 29 °C is characterized by both disorganized egg chambers in the ovarioles and also chorion-deficient oocytes. Both of these abnormalities may be the result of premature follicle cell death. The observations on 1(1)su(f)ts76a are consistent with the proposal that the similar allele, 1(1)ts726, is a cell-lethal mutation specifically affecting mitotically active cells.

scholarly journals Proteomic characterization of a temperature-sensitive conditional lethal in Drosophila melanogaster

Heredity ◽  
2009 ◽  
Vol 104 (2) ◽  
pp. 125-134 ◽  
Author(s):  
K S Pedersen ◽  
M C Codrea ◽  
C J Vermeulen ◽  
V Loeschcke ◽  
E Bendixen
Keyword(s):  
Drosophila Melanogaster ◽  
Temperature Sensitive
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