scholarly journals Cell culture: Fetal calf serum drought hits cell culture laboratories

Nature ◽  
1980 ◽  
Vol 285 (5760) ◽  
pp. 63-63 ◽  
Author(s):  
Ian Anderson
Keyword(s):  
Cell Culture ◽  
Fetal Calf Serum ◽  
Calf Serum

Effects of Zinc on Hepatopancreatic Cell Culture of Kuruma Prawn, Litopenaeus vannamei

2013 ◽  
Vol 864-867 ◽  
pp. 151-154
Author(s):  
Hong Wei Wang ◽  
Hai Ming Xu ◽  
Chun Long Zhao ◽  
Da Li ◽  
Hui Wang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Division ◽  
Litopenaeus Vannamei ◽  
Fetal Calf Serum ◽  
Cultured Cells ◽  
Calf Serum ◽  
Primary Cells

The hepatopancreatic cell culture of the kuruma prawn, Litopenaeusvannamei, was conducted to identify the effects of zinc on cell division. The culturesystem consists of medium 199 (M 199) supplemented with 0.060 mol/L NaCl,1.011g/L glucose, 1000 UI/ml penicillin, 1000 μg/ml treptomycin, 20% heatinactivated fetal calf serum (FCS) for primary cells and 10 % for subculture cells. TheRNA/DNA ratio in cultured cells was measured. The results show that the celldivision of cultured hepatopancreas cells in L. vannamei was increased by the optimalconcentration of Zn2+, 80 μg/L.

scholarly journals Whey and β-lactoglobulin: 2 milk by-products which can replace fetal calf serum in mouse hybridoma cell culture

1994 ◽  
Vol 74 (2) ◽  
pp. 127-137 ◽  
Author(s):  
J. Capiaumont ◽  
C. Legrand ◽  
B. Dousset ◽  
I. Parmentelot ◽  
G. Linden ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Hybridoma Cell Culture ◽  
Β Lactoglobulin ◽  
By Products

scholarly journals Eicosanoid content in fetal calf serum accounts for reproducibility challenges in cell culture

2020 ◽  
Author(s):  
Laura Niederstaetter ◽  
Benjamin Neuditschko ◽  
Julia Brunmair ◽  
Lukas Janker ◽  
Andrea Bileck ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Resolution ◽  
Fetal Calf Serum ◽  
Cultured Cells ◽  
High Resolution Mass Spectrometry ◽  
Calf Serum ◽  
Protein Composition ◽  
Efficient Uptake ◽  
U937 Macrophage

AbstractReproducibility issues regarding in vitro cell culture experiments are related to genetic fluctuations and batch-wise variations of biological materials such as fetal calf serum (FCS). Genome sequencing may control the former, while the latter may remain unrecognized. Using a U937 macrophage model for cell differentiation and inflammation, we investigated whether the formation of effector molecules was dependent on the FCS batch used for cultivation. High resolution mass spectrometry was used to identify FCS constituents and to explore their effects on cultured cells evaluating secreted cytokines, eicosanoids and other inflammatory mediators. Remarkably, the FCS eicosanoid composition showed more batch-dependent variations than the protein composition. Efficient uptake of fatty acids from medium by U937 macrophages and inflammation-induced release thereof was evidenced using C13-labelled arachidonic acid, highlighting rapid lipid metabolism. For functional testing, FCS batch-dependent nanomolar concentration differences of two selected eicosanoids, 5-HETE and 15-HETE, were balanced out by spiking in. Culturing U937 cells at these defined conditions indeed resulted in significant proteome alterations indicating HETE-induced PPARγ activation, independently corroborated by HETE-induced formation of peroxisomes observed by high-resolution microscopy. In conclusion, the present data demonstrate that FCS-contained eicosanoids, subject to substantial batch-wise variation, may modulate cellular effector functions in cell culture experiments.

Human derived alternatives to fetal calf serum in cell culture

Cytotherapy ◽  
2013 ◽  
Vol 15 (4) ◽  
pp. S16
Author(s):  
K. Ploederl ◽  
K. Höller ◽  
A. Lindenmair ◽  
D. Theiß ◽  
J. Kemptner ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fetal Calf Serum ◽  
Calf Serum

scholarly journals Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils.

2020 ◽  
Author(s):  
Razieh Alipour ◽  
Alimohammad Fatemi ◽  
Fereshteh Alsahebfosul ◽  
Alireza Andalib ◽  
Abbasali Pourazar
Keyword(s):  
Cell Culture ◽  
Fetal Calf Serum ◽  
Culture Media ◽  
Calf Serum ◽  
Cell Types ◽  
Autologous Serum ◽  
Autologous Plasma ◽  
Cd11b Expression ◽  
Suitable Alternative ◽  
Neutrophil Cell

Abstract Objective Currently the replacement of fetal calf serum (FCS) by a more suitable alternative is a sought aim in the field of tissue and cell culture research. Autologous plasma (AP) and especially autologous serum (AS) have been shown to be effective substitutes of FCS in culture media for some of cell types. Nevertheless, there is no comparative data on the most appropriate supplement for cell media in neutrophil studies, it is now unclear whether AP have relatively an equal, superior or inferior performance to FCS in neutrophil cell culture. In the present study, human blood neutrophils were isolated and cultured in FCS- or AP-supplemented medium. After 12, 36 and 60 hours of incubation, cell viability, oxidative burst and CD11b expression were determined by flow cytometry.Results Compared to the culture of neutrophils in FCS 10% medium, the culture of neutrophils in a medium with AP 10% could prolong their life span without affecting their function. The findings introduce AP as a better supplement for human neutrophil cell culture than FCS and propose a simple and economical procedure for neutrophil isolation and culture.

scholarly journals Effect of Cryopreservation on Different Passages of Porcine Dorsal Root Ganglion Cell Culture

2019 ◽  
Vol 5 ◽  
pp. 1
Author(s):  
Sabina Ali ◽  
Galina Bozhok ◽  
◽  
Keyword(s):  
Cell Culture ◽  
Primary Culture ◽  
Dorsal Root ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Relative Number ◽  
Cell Types ◽  
Dorsal Root Ganglion Cell ◽  
Neonatal Piglets ◽  
Culture Cells

The cell culture obtained from dorsal root ganglia (DRG) is a valuable model used in biology or medical research. However, the effect of cryopreservation on the properties of DRG-derived cell culture of different passages remains unclear up to date. The objective of the study is to assess the effect of cryopreservation with various concentrations of cryoprotectant dimethyl sulfoxide (DMSO) on the viability and morphological features of porcine neonatal DRG cell culture of different passages. Cell suspension was obtained from DRG of neonatal piglets and cultured in an α-MEM nutritional medium supplemented with 10% fetal calf serum (0, 1st, and 2nd passages). Cells of different passages were cryopreserved at a cooling rate of 0.5°C/min to – 20°C (step 1) and 1°C/min to – 80°C (step 2) followed by immersion into liquid nitrogen. Cryoprotective solutions based on α-MEM nutrient medium and 25% fetal calf serum (FCS) containing 5%, 7.5%, and 10% DMSO were used. It was established that the primary culture (passage 0) consisted of four cell morphological types: large rounded cell bodies of sensory neurons (SN) and three types of non-neuronal cells, namely, polygonal cells with pronounced elongated processes (type 1), spindle-shaped cells (type 2), and multipolar flattened fibroblastoid cells (type 3). As the number of passages increases, an elimination of SN from the culture, a decrease in the relative number of 1st and 2nd cell types, and expansion of 3rd cell type were observed. DRG cell culture had sufficiently high resistance to cryopreservation as cell viability was in the range from 83% to 90% using different concentrations of DMSO. The cells of passage 1 were more resistant to cryopreservation in comparison with primary culture cells (passage 0). The best result was achieved by freezing the culture of passage 1 in the cryoprotective medium with 7.5% DMSO, where 90.6% of viable cells were observed after thawing.

scholarly journals Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils.

2019 ◽  
Author(s):  
Razieh Alipour ◽  
Alimohammad Fatemi ◽  
Fereshteh Alsahebfosul ◽  
Alireza Andalib ◽  
Abbasali Pourazar
Keyword(s):  
Cell Culture ◽  
Fetal Calf Serum ◽  
Culture Media ◽  
Calf Serum ◽  
Cell Types ◽  
Autologous Serum ◽  
Autologous Plasma ◽  
Cd11b Expression ◽  
Suitable Alternative ◽  
Neutrophil Cell

Abstract Objective Currently the replacement of fetal calf serum (FCS) by a more suitable alternative is a sought aim in the field of tissue and cell culture research. Autologous plasma (AP) and especially autologous serum (AS) have been shown to be effective substitutes of FCS in culture media for some of cell types. Nevertheless, there is no comparative data on the most appropriate supplement for cell media in neutrophil studies, it is now unclear whether AP have relatively an equal, superior or inferior performance to FCS in neutrophil cell culture. In the present study, human blood neutrophils were isolated and cultured in FCS- or AP-supplemented medium. After 12, 36 and 60 hours of incubation, cell viability, oxidative burst and CD11b expression were determined by flow cytometry.Results Compared to the culture of neutrophils in FCS 10% medium, the culture of neutrophils in a medium with AP 10% could prolong their life span without affecting their function. The findings introduce AP as a better supplement for human neutrophil cell culture than FCS and propose a simple and economical procedure for neutrophil isolation and culture.

Elucidating the contribution of the elemental composition of fetal calf serum to antigenic expression of primary human umbilical-vein endothelial cells in vitro

2011 ◽  
Vol 31 (3) ◽  
pp. 199-210 ◽  
Author(s):  
Nicholas Bryan ◽  
Kirstie D. Andrews ◽  
Michael J. Loughran ◽  
Nicholas P. Rhodes ◽  
John A. Hunt
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Elemental Composition ◽  
Fetal Calf Serum ◽  
Umbilical Vein ◽  
Calf Serum ◽  
Human Umbilical Vein ◽  
Antigenic Expression ◽  
Umbilical Vein Endothelial Cells

One of the major obstacles to obtaining human cells of a defined and reproducible standard suitable for use as medical therapies is the necessity for FCS (fetal calf serum) media augmentation in routine cell culture applications. FCS has become the supplement of choice for cell culture research, as it contains an array of proteins, growth factors and essential ions necessary for cellular viability and proliferation in vitro. It is, however, a potential route for the introduction of zoonotic pathogens and makes defining the cell culture milieu impossible in terms of reproducibility, as the precise composition of each batch of serum not only changes but is in fact extremely variable. The present study determined the magnitude of donor variations in terms of elemental composition of FCS and the effect these variations had on the expression of a group of proteins associated with the antigenicity of primary human umbilical-vein endothelial cells, using a combination of ICPMS (inductively coupled plasma MS) and flow cytometry. Statistically significant differences were demonstrated for a set of trace elements in FCS, with correlations made to variations in antigenic expression during culture. The findings question in detail the suitability of FCS for the in vitro supplementation of cultures of primary human cells due to the lack of reproducibility and modulations in protein expression when cultured in conjunction with sera from xenogeneic donors.

scholarly journals Eicosanoid Content in Fetal Calf Serum Accounts for Reproducibility Challenges in Cell Culture

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 113
Author(s):  
Laura Niederstaetter ◽  
Benjamin Neuditschko ◽  
Julia Brunmair ◽  
Lukas Janker ◽  
Andrea Bileck ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Resolution ◽  
Fetal Calf Serum ◽  
Cultured Cells ◽  
High Resolution Mass Spectrometry ◽  
Calf Serum ◽  
Protein Composition ◽  
Efficient Uptake ◽  
U937 Macrophage

Reproducibility issues regarding in vitro cell culture experiments are related to genetic fluctuations and batch-wise variations of biological materials such as fetal calf serum (FCS). Genome sequencing may control the former, while the latter may remain unrecognized. Using a U937 macrophage model for cell differentiation and inflammation, we investigated whether the formation of effector molecules was dependent on the FCS batch used for cultivation. High resolution mass spectrometry (HRMS) was used to identify FCS constituents and to explore their effects on cultured cells evaluating secreted cytokines, eicosanoids, and other inflammatory mediators. Remarkably, the FCS eicosanoid composition showed more batch-dependent variations than the protein composition. Efficient uptake of fatty acids from the medium by U937 macrophages and inflammation-induced release thereof was evidenced using C13-labelled arachidonic acid, highlighting rapid lipid metabolism. For functional testing, FCS batch-dependent nanomolar concentration differences of two selected eicosanoids, 5-HETE and 15-HETE, were balanced out by spiking. Culturing U937 cells at these defined conditions indeed resulted in significant proteome alterations indicating HETE-induced PPARγ activation, independently corroborated by HETE-induced formation of peroxisomes observed by high-resolution microscopy. In conclusion, the present data demonstrate that FCS-contained eicosanoids, subject to substantial batch-wise variation, may modulate cellular effector functions in cell culture experiments.

scholarly journals Direct addition of poly-lysine or poly-ethylenimine to the medium, a simple alternative to plate pre-coating

2021 ◽  
Author(s):  
Alexander Faussner ◽  
Matthias Manfred Deininger ◽  
Chrisitan Weber ◽  
Sabine Steffens
Keyword(s):  
Cell Culture ◽  
Fetal Calf Serum ◽  
Cell Attachment ◽  
Calf Serum ◽  
Cationic Polymers ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
Direct Addition ◽  
293 Cells ◽  
Cost Efficient

For most cell culture experiments, it is indispensable that the cells are firmly anchored to the culture plates, tolerating several rinsing steps, and withstanding shear forces or temperature changes without detaching. For semi-adherent cells such as the very common HEK 293 cells, this could so far be obtained only by time-consuming plate pre-coating with cationic polymer solutions. We report here, that i) pre-coating with the cheaper poly-ethylenimine (PEI) works as well as the commonly used poly-D-lysine (PDL), but more importantly and novel ii) that simple direct addition of either PEI (1.5 µg/ml) or PDL (2 µg/ml) to the cell culture medium results in strongly anchored HEK 293 cells, indistinguishable from ones seeded on pre-coated plates. Therefore, the replacement of plate pre-coating by direct addition of either PEI or PDL gives comparable excellent results, but is highly labour-, time-, and cost-efficient. Interestingly, additional experiments in this context showed that strong cell attachment requires only cationic polymers but not fetal calf serum added to the medium. Fetal calf serum is, however, of course required for further maintenance and growth of the cells.

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