Role of the blastocoele microenvironment in early mouse embryo differentiation

Nature ◽  
1980 ◽  
Vol 284 (5756) ◽  
pp. 550-552 ◽  
Author(s):  
Roger A. Pedersen ◽  
Akiko I. Spindle
Keyword(s):  
Mouse Embryo ◽  
Early Mouse Embryo ◽  
Early Mouse

The role of cell adhesion in the synchronization and orientation of polarization in 8-cell mouse blastomeres

Development ◽  
1986 ◽  
Vol 93 (1) ◽  
pp. 239-255
Author(s):  
Martin H. Johnson ◽  
Bernard Maro ◽  
Masatoshi Takeichi
Keyword(s):  
Cell Adhesion ◽  
Mouse Embryo ◽  
Normal Development ◽  
Early Event ◽  
Early Mouse Embryo ◽  
Adhesion System ◽  
Dependent Cell ◽  
Temporal And Spatial ◽  
Early Mouse

A detailed investigation into the activity of the homotypic, Ca2+-dependent cell-cell adhesion system (CDS) in the early mouse embryo has revealed its involvement in (i) the synchronizing of the time of polarization of 8-cell blastomeres, and (ii) the orienting of the axis of polarization. Since polarization marks an important and early event in the process of cell diversification in the mouse embryo, it is concluded that the CDS provides an important component of the system by which the temporal and spatial elements of normal development are integrated.

Cell-matrix interactions in the early mouse embryo

1992 ◽  
Vol 50 (1) ◽  
pp. 600-601
Author(s):  
A.E. Sutherland ◽  
P.G. Calarco ◽  
C.H. Damsky
Keyword(s):  
Mouse Embryo ◽  
Giant Cells ◽  
Blastocyst Stage ◽  
Integrin Subunit ◽  
Β1 Integrin ◽  
Cell Stage ◽  
Early Mouse Embryo ◽  
Migratory Activity ◽  
Early Mouse ◽  
Cell Matrix Interactions

Cell-extracellular matrix (ECM) interactions mediated by the integrin family of receptors are critical for morphogenesis and may also play a regulatory role in differentiation during early development. We have examined the onset of expression of individual integrin subunit proteins in the early mouse embryo, and their roles in early morphogenetic events. As detected by immunoprecipitation, the α6, αV, β1, and β3 subunits are detected as early as the 4-cell stage, α5 at the hatched blastocyst stage and αl and α3 following blastocyst attachment. We tested the role of these integrins in the attachment and migratory activity of two cell populations of the early mouse embryo: the trophoblast giant cells, which invade the uterine stroma and ultimately contribute to the chorio-allantoic placenta, and the parietal endoderm, which migrates over the inner surface of the trophoblast and ultimately forms Reichert's membrane and the parietal yolk sac. Experiments were done in serum-free medium on substrates coated with laminin (Ln) and fibronectin (Fn). Trophoblast outgrowth occurs on Ln and its E8 fragment (long arm), but not on the E1’ fragment (cross region) (Figs. 1, 2 ). This outgrowth is inhibited by anti-E8, anti-Ln, and by the anti-β1 family antiserum anti-ECMR, but not by anti-αV or the function-perturbing GoH3 antibody that recognizes the α6/β1 integrin, a major Ln (E8) receptor. This suggests that trophoblast outgrowth on Ln or E8 is mediated by a different β1 integrin such as α3/β1. Early stages of trophoblast outgrowth (up to 48 hours) on Fn are inhibited by anti-Fn and by function-perturbing anti-αV antibodies, whereas at later times outgrowth becomes insensitive to anti-αV but remains sensitive to the anti-β1 family antiserum anti-ECMr, indicating that trophoblast cells modulate their interaction with Fn during outgrowth. Trophoblast outgrowth on vitronectin (Vn) is sensitive to anti-αV antibodies throughout the 5-day period examined.

The Expression of X-Linked Phosphoglycerate Kinase in the Early Mouse Embryo

1982 ◽  
Vol 23 (1-3) ◽  
pp. 141-144 ◽  
Author(s):  
W.K.G. Krietsch ◽  
R. Fundele ◽  
G.W.K. Kuntz ◽  
M. Fehlau ◽  
K. Bürki ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Phosphoglycerate Kinase ◽  
Early Mouse Embryo ◽  
Early Mouse
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