Correlation between fragmented immunoglobulin genes and heavy chain deletion mutants

Nature ◽  
1979 ◽  
Vol 281 (5732) ◽  
pp. 600-602 ◽  
Author(s):  
Blas Frangione ◽  
Edward C. Franklin
Keyword(s):  
Heavy Chain ◽  
Deletion Mutants ◽  
Immunoglobulin Genes

Nuclear transcripts of mouse heavy chain immunoglobulin genes contain only the expressed class of C-region sequences

Science ◽  
1979 ◽  
Vol 204 (4397) ◽  
pp. 1087-1088 ◽  
Author(s):  
K. Marcu ◽  
U Schibler ◽  
R. Perry
Keyword(s):  
Heavy Chain ◽  
Immunoglobulin Genes

Anti-CD40 plus interleukin-4-activated human naive B cell lines express unmutated immunoglobulin genes with intraclonal heavy chain isotype variability

1995 ◽  
Vol 25 (3) ◽  
pp. 733-737 ◽  
Author(s):  
Laurent Galibert ◽  
Joelle van Dooren ◽  
Isabelle Durand ◽  
Françoise Rousset ◽  
Royston Jefferis ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Lines ◽  
Heavy Chain ◽  
Interleukin 4 ◽  
Immunoglobulin Genes

scholarly journals Genes activated in the presence of an immunoglobulin enhancer or promoter are negatively regulated by a T-lymphoma cell line.

1988 ◽  
Vol 8 (5) ◽  
pp. 1932-1939 ◽  
Author(s):  
D M Zaller ◽  
H Yu ◽  
L A Eckhardt
Keyword(s):  
Heavy Chain ◽  
Immunoglobulin Heavy Chain ◽  
Negative Control ◽  
Lymphoma Cell ◽  
Chain Gene ◽  
Immunoglobulin Genes ◽  
Heavy Chain Gene ◽  
Immunoglobulin Heavy Chain Gene ◽  
Specific Expression ◽  
T Lymphoma

The tissue-specific expression of immunoglobulin genes can be partially explained by a requirement for activating factors found only in B lymphocytes and their derivatives. However, loss of immunoglobulin expression upon fusion of an immunoglobulin-producing myeloma cell with a T lymphoma cell (BW5147) or fibroblast (L cell) suggests that negatively acting factors also play a role in the tissue specificity of immunoglobulin genes. Expression of a cloned immunoglobulin heavy-chain gene introduced into myeloma cells was suppressed after fusion of the myeloma transformants with BW5147. The presence of either the immunoglobulin heavy-chain enhancer or promoter conferred suppression, under similar conditions, upon a heterologous gene that is normally expressed in both B and T lymphocytes. These immunoglobulin heavy-chain gene control regions, or gene modifications induced by them, are subject to negative control by T-lymphocyte-derived factors.

Biochemical genetics of the mouse IgM system

1984 ◽  
Vol 62 (4) ◽  
pp. 217-224 ◽  
Author(s):  
Marc J. Shulman ◽  
Robert G. Hawley ◽  
Atsuo Ochi ◽  
W. O. T. Baczynsky ◽  
Catherine Collins ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Heavy Chain ◽  
Mutant Cell ◽  
Immunoglobulin M ◽  
Structural Basis ◽  
Deletion Mutants ◽  
Transfer System ◽  
Biochemical Genetics ◽  
Chain Synthesis

Using a mouse hybridoma system, we have developed methods of isolating a variety of mutant cell lines in which immunoglobulin function or synthesis is defective. The analysis of mutants defective in κ chain synthesis has defined a class of murine transposons. The deletion mutants produce immunoglobulin M (IgM) bearing μ heavy chain fragments and provide information on the requirements of IgM assembly and μ gene expression. We also describe a transfer system for the μ and κ genes which will be useful in analyzing the structural basis of IgM function.

scholarly journals Nucleotide sequences of Immunoglobulin μ heavy chain deletion mutants

1983 ◽  
Vol 11 (21) ◽  
pp. 7471-7485 ◽  
Author(s):  
W.O.T. Baczynsky ◽  
Shunji Sugii ◽  
Helios Murialdo ◽  
Nancy Pennell ◽  
Catherine Filkin ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Nucleotide Sequences ◽  
Deletion Mutants

scholarly journals The immunoglobulin heavy chain locus contains another B-cell-specific 3' enhancer close to the alpha constant region.

1993 ◽  
Vol 13 (3) ◽  
pp. 1547-1553 ◽  
Author(s):  
P Matthias ◽  
D Baltimore
Keyword(s):  
Transcription Factors ◽  
B Cell ◽  
Heavy Chain ◽  
Variable Region ◽  
Immunoglobulin Heavy Chain ◽  
Immunoglobulin Genes ◽  
Constant Region ◽  
Transcriptional Enhancer ◽  
Additional Control ◽  
Complex Regulation

The transcription of immunoglobulin genes is controlled by variable region promoters and by enhancers, both of which are lymphoid specific. Because immunoglobulin genes are subject to an extremely complex regulation, we anticipated that there might be additional control elements for these genes. We therefore sought additional enhancers and demonstrate here that there is indeed another weak transcriptional enhancer just 3' to the mouse alpha constant region. This novel immunoglobulin enhancer is lymphoid specific and at two positions can bind members of the Oct family of transcription factors.

The variability, arrangement, and rearrangement of immunoglobulin genes

1980 ◽  
Vol 58 (3) ◽  
pp. 176-187 ◽  
Author(s):  
T. H. Rabbitts ◽  
P. H. Hamlyn ◽  
G. Matthyssens ◽  
B. A. Roe
Keyword(s):  
Heavy Chain ◽  
Cdna Clone ◽  
Variable Region ◽  
Immunoglobulin Genes ◽  
Constant Region ◽  
Cross Hybridization ◽  
Heavy Chain Variable Region ◽  
Human Dna ◽  
V Genes

The multiplicity of heavy-chain variable-region (VH) genes in mouse and human DNA has been estimated using a mouse heavy- (H) chain cDNA clone. We found about 10 hybridization components in mouse DNA and about 20 components in human DNA. Cross-hybridization studies of variable region (V) genes indicate that these components represent the numbers of genes within the VH subgroups of each of these species. The arrangement and rearrangement of the H-chain γ subclasses have been studied in order to assess possible mechanisms of the H-chain switch. Evidence has been found for rearrangement events involving the γ2a and γ2b constant-region (CH) genes in DNA from cells making IgG2a and IgG2b respectively. In addition we found that cells making IgG2a lack detectable genes for γ1 and γ2b. Both sets of observations are discussed in relation to H-chain diversity and the switch.

THE ORGANIZATION AND REARRANGEMENT OF HEAVY CHAIN IMMUNOGLOBULIN GENES IN MICE

1979 ◽  
pp. 393-406 ◽  
Author(s):  
M. Davis ◽  
P. Early ◽  
K. Calame ◽  
D. Livant ◽  
L. Hood
Keyword(s):  
Heavy Chain ◽  
Immunoglobulin Genes
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