Opposite effects of intracellular Ca2+ and glucose on K+ permeability of pancreatic islet cells

Nature ◽  
1979 ◽  
Vol 280 (5717) ◽  
pp. 66-68 ◽  
Author(s):  
J. C. HENQUIN
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
K Permeability

scholarly journals Metabolic control of the potassium permeability in pancreatic islet cells

1980 ◽  
Vol 186 (2) ◽  
pp. 541-550 ◽  
Author(s):  
Jean-Claude Henquin
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Reduced Glutathione ◽  
Pancreatic Islet Cells ◽  
Major Step ◽  
Metabolic Degradation ◽  
Glucose Metabolites ◽  
Effect Of Glucose ◽  
Phenazine Methosulphate ◽  
K Permeability

The K+ permeability of pancreatic islet cells was studied by monitoring the efflux of 86Rb+ (used as tracer for K+) from perifused rat islets and measuring the uptake of 42K+. Glucose markedly and reversibly decreased 86Rb+ efflux from islet cells and this effect was antagonized by inhibitors of the metabolic degradation of the sugar, i.e. mannoheptulose, iodoacetate, glucosamine and 2-deoxyglucose. Among glucose metabolites, glyceraldehyde reduced the K+ permeability even more potently than did glucose itself; pyruvate and lactate alone exhibited only a small effect, but potentiated that of glucose. Other metabolized sugars, like mannose, glucosamine and N-acetylglucosamine, also decreased 86Rb+ efflux from islet cells. Fructose was effective only in the presence of glucose. Non-metabolized sugars like galactose, 2-deoxyglucose and 3-O-methylglucose had no effect. The changes in K+ permeability by agents known to modify the concentrations of nicotinamide nucleotides, glutathione or ATP in islet cells were also studied. Increasing NAD(P)H concentrations in islet cells by pentobarbital rapidly and reversibly reduced 86Rb+ efflux; exogenous reduced glutathione produced a similar though weaker effect. By contrast, oxidizing nicotinamide nucleotides with phenazine methosulphate or Methylene Blue, or oxidizing glutathione by t-butyl hydroperoxide increased the K+ permeability of islet cells. Uncoupling the oxidative phosphorylations with dicumarol also augmented 86Rb+ efflux markedly. In the absence of glucose, but not in its presence, methylxanthines reduced 86Rb+ efflux from the islets; such was not the case for cholera toxin or dibutyryl cyclic AMP. Glucose and glyceraldehyde had no effect on 42K+ uptake after a short incubation (10min), but augmented it after 60min; the effect of glucose was suppressed by mannoheptulose and not mimicked by 3-O-methylglucose. The results clearly establish the importance of the metabolic degradation of glucose and other substrates for the control of the K+ permeability in pancreatic islet cells and support the concept that a decrease in the K+ permeability represents a major step of the B-cell response to physiological stimulation.

Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts

Diabetes ◽  
1980 ◽  
Vol 29 (6) ◽  
pp. 497-500 ◽  
Author(s):  
P. Meda ◽  
E. L. Hooghe-Peters ◽  
L. Orci
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Monolayer Cultures

Use of liposomes to introduce substances into pancreatic islet cells

Diabetes ◽  
1988 ◽  
Vol 37 (8) ◽  
pp. 1123-1128
Author(s):  
N. Welsh ◽  
A. Hallberg ◽  
S. Sandler ◽  
C. Hellerstrom
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Superfusion of dissociated pancreatic islet cells attached to Cytodex beads

Diabetes ◽  
1982 ◽  
Vol 31 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Y. Spiess ◽  
M. A. Smith ◽  
W. Vale
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Efficient gene transfer to dispersed human pancreatic islet cells in vitro using adenovirus-polylysine/DNA complexes or polycationic liposomes

Diabetes ◽  
1996 ◽  
Vol 45 (9) ◽  
pp. 1197-1203 ◽  
Author(s):  
J. Saldeen ◽  
D. T. Curiel ◽  
D. L. Eizirik ◽  
A. Andersson ◽  
E. Strandell ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Dna Complexes ◽  
Human Pancreatic Islet ◽  
Efficient Gene

Effect of sulfur dioxide exposure on histopathology and morphometry of pancreatic islet cells and glycemic indices in Wistar rats

2021 ◽  
Author(s):  
Razieh Musapour Soltan-Abad ◽  
Sorayya Kheirouri ◽  
Mehran Mesgari Abbasi ◽  
Mohammad Alizadeh ◽  
Monireh Khordadmehr
Keyword(s):  
Sulfur Dioxide ◽  
Pancreatic Islet ◽  
Wistar Rats ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Glycemic Indices
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