DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI

Nature ◽  
10.1038/27952 ◽  
1998 ◽  
Vol 394 (6688) ◽  
pp. 96-101 ◽  
Author(s):  
Karen E. Flick ◽  
Melissa S. Jurica ◽  
Raymond J. Monnat ◽  
Barry L. Stoddard
Keyword(s):  
Dna Binding ◽  
Homing Endonuclease ◽  
Nuclear Intron ◽  
Dna Binding And Cleavage

scholarly journals The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage

2007 ◽  
Vol 35 (10) ◽  
pp. 3262-3271 ◽  
Author(s):  
Jesús Prieto ◽  
Pilar Redondo ◽  
Daniel Padró ◽  
Sylvain Arnould ◽  
Jean-Charles Epinat ◽  
...  
Keyword(s):  
Dna Binding ◽  
Homing Endonuclease ◽  
Terminal Loop ◽  
Site Recognition ◽  
Dna Binding And Cleavage

DNA Binding and Cleavage by the HNH Homing Endonuclease I-HmuI

2004 ◽  
Vol 342 (1) ◽  
pp. 43-56 ◽  
Author(s):  
Betty W. Shen ◽  
Markus Landthaler ◽  
David A. Shub ◽  
Barry L. Stoddard
Keyword(s):  
Dna Binding ◽  
Homing Endonuclease ◽  
Dna Binding And Cleavage

Synthesis, Structural, DNA Binding and Cleavage Studies of Cu(II) Complexes Containing Benzothiazole Cored Schiff Bases

2016 ◽  
Vol 26 (6) ◽  
pp. 2151-2163 ◽  
Author(s):  
Somapangu Tejaswi ◽  
Marri Pradeep Kumar ◽  
Aveli Rambabu ◽  
Narendrula Vamsikrishna ◽  
Shivaraj
Keyword(s):  
Dna Binding ◽  
Schiff Bases ◽  
Dna Binding And Cleavage

scholarly journals Cas12a is a dynamic and precise RNA-guided nuclease without off-target activity on λ-DNA

2021 ◽  
Author(s):  
Bijoya Paul ◽  
Loic Chaubet ◽  
Emma Verver ◽  
Guillermo Montoya
Keyword(s):  
Dna Binding ◽  
Genome Editing ◽  
Optical Tweezers ◽  
Target Site ◽  
Target Dna ◽  
Multiple Binding ◽  
Target Sites ◽  
Dna Binding And Cleavage ◽  
Target Activity ◽  
Insight Into

Cas12a is an RNA-guided endonuclease that is emerging as a powerful genome-editing tool. Here we combined optical tweezers with fluorescence to monitor Cas12a binding onto λ-DNA, providing insight into its DNA binding and cleavage mechanisms. At low forces Cas12a binds DNA specifically with two off-target sites, while at higher forces numerous binding events appear driven by the mechanical distortion of the DNA and partial matches to the crRNA. Despite the multiple binding events, cleavage is only observed on the target site at low forces, when the DNA is flexible. Activity assays show that the preferential off-target sites are not cleaved, and the λ-DNA is severed at the target site. This precision is also observed in Cas12a variants where the specific dsDNA and the unspecific ssDNA cleavage are dissociated or nick the target DNA. We propose that Cas12a and its variants are precise endonucleases that efficiently scan the DNA for its target but only cleave the selected site in the λ-DNA.

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