Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites

Nature ◽  
1978 ◽  
Vol 272 (5651) ◽  
pp. 375-377 ◽  
Author(s):  
BRUNO GRONENBORN ◽  
JOACHIM MESSING
Keyword(s):  
Restriction Endonuclease ◽  
Cleavage Sites ◽  
Single Stranded Dna ◽  
Restriction Endonuclease Cleavage

Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei

1982 ◽  
Vol 2 (7) ◽  
pp. 845-852
Author(s):  
K D Stuart ◽  
S B Gelvin
Keyword(s):  
Restriction Endonuclease ◽  
Trypanosoma Brucei ◽  
Variable Region ◽  
Cleavage Sites ◽  
Kinetoplast Dna ◽  
Coding Sequences ◽  
Procyclic Form ◽  
Restriction Endonuclease Cleavage ◽  
Mitochondrial Respiratory

Over 80% of the maxicircle and numerous minicircles of Trypanosoma brucei kinetoplast DNA have been cloned. The uncloned maxicircle segment contains few restriction endonuclease cleavage sites, varies in size among strains, and may be unstable in conventional cloning systems. cDNA prepared to bloodstream or procyclic trypomastigote RNA hybridized to all but one maxicircle segment, but did not hybridize to minicircles. Fourteen maxicircle transcripts were detected in RNA from both bloodstream and procyclic trypomastigotes. The coding sequences for these transcripts were localized and account for most of the maxicircle. One region of the maxicircle, which borders the variable region, was not found to be transcribed. We conclude that the maxicircle is largely but not completely transcribed in both bloodstream and procyclic trypomastigotes, whereas minicircle transcription is minimal or absent in these stages. Qualitative transcriptional differences which could account for mitochondrial respiratory differences between the bloodstream and procyclic trypomastigotes were not observed.

scholarly journals Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei.

1982 ◽  
Vol 2 (7) ◽  
pp. 845-852 ◽  
Author(s):  
K D Stuart ◽  
S B Gelvin
Keyword(s):  
Restriction Endonuclease ◽  
Trypanosoma Brucei ◽  
Variable Region ◽  
Cleavage Sites ◽  
Kinetoplast Dna ◽  
Coding Sequences ◽  
Procyclic Form ◽  
Restriction Endonuclease Cleavage ◽  
Mitochondrial Respiratory

Over 80% of the maxicircle and numerous minicircles of Trypanosoma brucei kinetoplast DNA have been cloned. The uncloned maxicircle segment contains few restriction endonuclease cleavage sites, varies in size among strains, and may be unstable in conventional cloning systems. cDNA prepared to bloodstream or procyclic trypomastigote RNA hybridized to all but one maxicircle segment, but did not hybridize to minicircles. Fourteen maxicircle transcripts were detected in RNA from both bloodstream and procyclic trypomastigotes. The coding sequences for these transcripts were localized and account for most of the maxicircle. One region of the maxicircle, which borders the variable region, was not found to be transcribed. We conclude that the maxicircle is largely but not completely transcribed in both bloodstream and procyclic trypomastigotes, whereas minicircle transcription is minimal or absent in these stages. Qualitative transcriptional differences which could account for mitochondrial respiratory differences between the bloodstream and procyclic trypomastigotes were not observed.

Survey and mapping of restriction endonuclease cleavage sites in bacteriophage T7 DNA

1979 ◽  
Vol 135 (4) ◽  
pp. 907-915 ◽  
Author(s):  
Alan H. Rosenberg ◽  
Martha N. Simon ◽  
F.William Studier ◽  
Richard J. Roberts
Keyword(s):  
Restriction Endonuclease ◽  
Bacteriophage T7 ◽  
Cleavage Sites ◽  
Restriction Endonuclease Cleavage

scholarly journals Additional Restriction Endonuclease Cleavage Sites on the Bacteriophage P22 Genome

1983 ◽  
Vol 45 (2) ◽  
pp. 864-867 ◽  
Author(s):  
Sherwood Casjens ◽  
Melody Hayden ◽  
Ethel Jackson ◽  
Robert Deans
Keyword(s):  
Restriction Endonuclease ◽  
Additional Restriction ◽  
Cleavage Sites ◽  
Bacteriophage P22 ◽  
Restriction Endonuclease Cleavage
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