Photo-oxidation of water in phospholipid bilayer membranes containing chlorophyll a

Nature ◽  
1977 ◽  
Vol 265 (5590) ◽  
pp. 187-189 ◽  
Author(s):  
Y. TOYOSHIMA ◽  
M. MORINO ◽  
H. MOTOKI ◽  
M. SUKIGARA
Keyword(s):  
Chlorophyll A ◽  
Phospholipid Bilayer ◽  
Bilayer Membranes ◽  
Photo Oxidation

scholarly journals Fusion of phospholipid vesicles with planar phospholipid bilayer membranes. II. Incorporation of a vesicular membrane marker into the planar membrane.

1980 ◽  
Vol 75 (3) ◽  
pp. 251-270 ◽  
Author(s):  
F S Cohen ◽  
J Zimmerberg ◽  
A Finkelstein
Keyword(s):  
Divalent Cations ◽  
Phospholipid Bilayer ◽  
Phospholipid Vesicles ◽  
Osmotic Gradient ◽  
Bilayer Membranes ◽  
Fusion Procedure ◽  
Vesicular Membrane ◽  
Membrane Contact ◽  
Planar Membrane ◽  
Planar Membranes

Fusion of multilamellar phospholipid vesicles with planar phospholipid bilayer membranes was monitored by the rate of appearance in the planar membrane of an intrinsic membrane protein present in the vesicle membranes. An essential requirement for fusion is an osmotic gradient across the planar membrane, with the cis side (the side containing the vesicles) hyperosmotic to the opposite (trans) side; for substantial fusion rates, divalent cation must also be present on the cis side. Thus, the low fusion rates obtained with 100 mM excess glucose in the cis compartment are enhanced orders of magnitude by the addition of 5-10 mM CaCl2 to the cis compartment. Conversely, the rapid fusion rates induced by 40 mM CaCl2 in the cis compartment are completely suppressed when the osmotic gradient (created by the 40 mM CaCl2) is abolished by addition of an equivalent amount of either CaCl2, NaCl, urea, or glucose to the trans compartment. We propose that fusion occurs by the osmotic swelling of vesicles in contact with the planar membrane, with subsequent rupture of the vesicular and planar membranes in the region of contact. Divalent cations catalyze this process by increasing the frequency and duration of vesicle-planar membrane contact. We argue that essentially this same osmotic mechanism drives biological fusion processes, such as exocytosis. Our fusion procedure provides a general method for incorporating and reconstituting transport proteins into planar phospholipid bilayer membranes.

scholarly journals High Pressure Bioscience. Phospholipid Bilayer Membranes under High Pressure.

1999 ◽  
Vol 9 (3) ◽  
pp. 213-220 ◽  
Author(s):  
Shoji KANESHINA ◽  
Hitoshi MATSUKI ◽  
Hayato ICHIMORI
Keyword(s):  
High Pressure ◽  
Phospholipid Bilayer ◽  
Bilayer Membranes

scholarly journals Diffusion in phospholipid bilayer membranes: dual-leaflet dynamics and the roles of tracer–leaflet and inter-leaflet coupling

2014 ◽  
Vol 470 (2167) ◽  
pp. 20130843 ◽  
Author(s):  
Reghan J. Hill ◽  
Chih-Ying Wang
Keyword(s):  
Lateral Diffusion ◽  
Bilayer Membrane ◽  
Tracer Diffusion ◽  
Phospholipid Bilayer ◽  
Supported Membranes ◽  
Bilayer Membranes ◽  
Lubrication Film ◽  
Lipid Assemblies ◽  
Slip Coefficients ◽  
Made In

A variety of observations—sometimes controversial—have been made in recent decades when attempting to elucidate the roles of interfacial slip on tracer diffusion in phospholipid membranes. Evans–Sackmann theory (1988) has furnished membrane viscosities and lubrication-film thicknesses for supported membranes from experimentally measured lateral diffusion coefficients. Similar to the Saffman and Delbrück model, which is the well-known counterpart for freely supported membranes, the bilayer is modelled as a single two-dimensional fluid. However, the Evans–Sackman model cannot interpret the mobilities of monotopic tracers, such as individual lipids or rigidly bound lipid assemblies; neither does it account for tracer–leaflet and inter-leaflet slip. To address these limitations, we solve the model of Wang and Hill, in which two leaflets of a bilayer membrane, a circular tracer and supports are coupled by interfacial friction, using phenomenological friction/slip coefficients. This furnishes an exact solution that can be readily adopted to interpret the mobilities of a variety of mosaic elements—including lipids, integral monotopic and polytopic proteins, and lipid rafts—in supported bilayer membranes.

Thermodynamics of glycophorin in phospholipid bilayer membranes

Biochemistry ◽  
1987 ◽  
Vol 26 (7) ◽  
pp. 1909-1917 ◽  
Author(s):  
A. Leo MacDonald ◽  
David A. Pink
Keyword(s):  
Phospholipid Bilayer ◽  
Bilayer Membranes
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