Magnesium required for serum-stimulation of growth in cultures of chick embryo fibroblasts

Nature ◽  
1976 ◽  
Vol 263 (5573) ◽  
pp. 143-145 ◽  
Author(s):  
JAMES KAMINE ◽  
HARRY RUBIN
Keyword(s):  
Chick Embryo ◽  
Serum Stimulation ◽  
Embryo Fibroblasts ◽  
Stimulation Of

Growth Hormone-Dependent Serum Stimulation of DNA Synthesis in Chick Embryo Fibroblasts in Culture12

Endocrinology ◽  
1975 ◽  
Vol 96 (1) ◽  
pp. 193-198 ◽  
Author(s):  
KENNETH L. COHEN ◽  
PATRICIA A. SHORT ◽  
S. PETER NISSLEY
Keyword(s):  
Growth Hormone ◽  
Chick Embryo ◽  
Dna Synthesis ◽  
Serum Stimulation ◽  
Embryo Fibroblasts ◽  
Stimulation Of

The effect of exogenous RNA extract on chick embryo fibroblasts in vitro

Development ◽  
1970 ◽  
Vol 23 (2) ◽  
pp. 509-517
Author(s):  
A. Sann ◽  
D. Sharp ◽  
J. McKenzie
Keyword(s):  
Cell Differentiation ◽  
Chick Embryo ◽  
Morphological Changes ◽  
Bacterial Cells ◽  
Baby Hamster Kidney ◽  
Exogenous Rna ◽  
Embryo Fibroblasts ◽  
Stimulation Of ◽  
Rna Extract

It is extremely difficult, if not impossible, to reconcile the conflicting claims of those who have treated different cells and tissues with exogenous RNA. Some authors (e.g. Niu, Cordova & Niu, 1961; Niu, Cordova & Radbill, 1962) maintain that RNA extracts alter the course of cell differentiation to conform in morphological terms to the source of the RNA; in the same vein, Amos, Askonas & Soeiro (1964) have shown that, under certain conditions, RNA from mouse and bacterial cells can stimulate chick embryo fibroblasts to synthesize protein related antigenically to the origin of the RNA. Shepley, Ambrose & Kirby (1965), however, obtained stimulation of growth with permanent morphological changes in baby hamster kidney fibroblasts by the addition of RNA from a variety of sources.

scholarly journals Counter-transport in chick embryo fibroblasts. A significant factor in measurement of glucose entry

1982 ◽  
Vol 206 (2) ◽  
pp. 301-309 ◽  
Author(s):  
R J Gay ◽  
H Amos
Keyword(s):  
Chick Embryo ◽  
Glucose Transport ◽  
Cell Cultures ◽  
Chicken Embryo ◽  
Primary Cell ◽  
Primary Cell Cultures ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts ◽  
Stimulation Of

Enhanced rates of carrier-mediated 3-O-methyl-D-glucose (0.1 mM) transport were observed in primary cell cultures of chicken embryo fibroblasts deprived of glucose for 1 day. The addition of 5.5 mM-glucose, glucosamine or 2-deoxy-D-glucose for 15 min (37 degrees C) to glucose-starved cultures followed by washing and immediate measurement of 3-O-methyl-D-glucose transport resulted in an apparent further stimulation of transport. Transport stimulation increased with increasing concentrations of the added preincubation sugar and was observed at test concentrations ranging from 0.1 mM- to 10 mM-3-O-methyl-D-glucose. This enhancement occurred when the preloaded sugar was rapidly effluxing from cells and was eliminated by allowing cultures to incubate in buffer without sugar for 30 min (37 degrees C) after the removal of hexose and before measuring transport. A transient overshoot in the cumulative uptake of 3-O-methyl-D-glucose was observed in glucose-starved cultures that were pre-incubated in the presence of 55 mM-glucose or -glucosamine for 15 min (37 degrees C). These data suggest that counter-transport accounts for the apparent enhancement of glucose-transport capability observed in glucose-starved cells when they are briefly re-exposed to hexose.

scholarly journals Expression of the v-src or v-fps oncogene increases fructose 2,6-bisphosphate in chick-embryo fibroblasts. Novel mechanism for the stimulation of glycolysis by retroviruses

1986 ◽  
Vol 236 (2) ◽  
pp. 595-599 ◽  
Author(s):  
L Bosca ◽  
M Mojena ◽  
J Ghysdael ◽  
G G Rousseau ◽  
L Hue
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Kinase Activation ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts ◽  
Rate Limiting ◽  
Stimulation Of

The concentration of fructose 2,6-bisphosphate and the activity of 6-phosphofructo-2-kinase are increased after infection of chick-embryo fibroblasts with the Rous sarcoma virus, or with a temperature-sensitive mutant of this virus at the permissive, but not at the non-permissive, temperature. This is observed after transformation by retroviruses carrying either the v-src or v-fps, but not the v-mil and/or v-myc, oncogenes. Comparison of the effects of the Rous sarcoma virus with those of phorbol myristate acetate on fructose 2,6-bisphosphate suggests that both result from the stimulation of a step which is rate-limiting for 6-phosphofructo-2-kinase activation and which is also controlled by protein kinase C.

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