Membrane action of DMSO and other chemical inducers of Friend leukaemic cell differentiation

Nature ◽  
1976 ◽  
Vol 262 (5567) ◽  
pp. 360-363 ◽  
Author(s):  
Gary H. Lyman ◽  
Harvey D. Preisler ◽  
Demetrios Papahadjopoulos
Keyword(s):  
Cell Differentiation ◽  
Leukaemic Cell ◽  
Membrane Action ◽  
Chemical Inducers

scholarly journals Relationship between globin mRNA accumulation and commitment to terminal cell differentiation in inducer sensitive and resistant erythroleukemia variants

Blood ◽  
1990 ◽  
Vol 76 (2) ◽  
pp. 302-306
Author(s):  
N Weich ◽  
PA Marks ◽  
RA Rifkind
Keyword(s):  
Cell Differentiation ◽  
Erythroid Differentiation ◽  
Terminal Cell ◽  
Globin Mrna ◽  
Mrna Accumulation ◽  
Chemical Inducers ◽  
Transformed Cell Line ◽  
Increased Sensitivity ◽  
Differentiation Inducers ◽  
Murine Erythroleukemia

Abstract The relationship between the kinetics of commitment to terminal cell differentiation and the rates of accumulation of globin mRNA has been examined during the induction of erythroid differentiation by polar/apolar chemical inducers in murine erythroleukemia cells (MELC), under conditions of more and less rapid commitment. Two differentiation inducers and three MELC variants have been studied. Hexamethylene bisacetamide (HMBA) initiates more rapid commitment than does dimethylsulfoxide (Me2SO). MELC variant DR10 is resistant to induction by Me2SO and responds sluggishly to HMBA, in comparison with the DS19- Sc9 variant. V3.17, an MELC variant resistant to low concentrations of vincristine, shows increased sensitivity to the inducers and an accelerated rate of commitment to terminal differentiation compared with DS19-Sc9. It is demonstrated that commitment and the actual expression of differentiation, as measured by the accumulation of alpha- , beta maj-, and beta min-globin mRNA, are temporally coordinated functions during induced differentiation of a transformed cell line by exposure to polar/apolar agents.

scholarly journals Conducting Polymer Mediated Electrical Stimulation Induces Multilineage Differentiation with Robust Neuronal Fate Determination of Human Induced Pluripotent Stem Cells

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 658 ◽  
Author(s):  
Eva Tomaskovic-Crook ◽  
Qi Gu ◽  
Siti N Abdul Rahim ◽  
Gordon G Wallace ◽  
Jeremy M Crook
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Cell Differentiation ◽  
Pluripotent Stem Cells ◽  
Stem Cell Differentiation ◽  
Chemical Inducers ◽  
Neuronal Fate ◽  
Induced Pluripotent

Electrical stimulation is increasingly being used to modulate human cell behaviour for biotechnological research and therapeutics. Electrically conductive polymers (CPs) such as polypyrrole (PPy) are amenable to in vitro and in vivo cell stimulation, being easy to synthesise with different counter ions (dopants) to augment biocompatibility and cell-effects. Extending our earlier work, which showed that CP-mediated electrical stimulation promotes human neural stem cell differentiation, here we report using electroactive PPy containing the anionic dopant dodecylbenzenesulfonate (DBS) to modulate the fate determination of human induced pluripotent stem cells (iPSCs). Remarkably, the stimulation without conventional chemical inducers resulted in the iPSCs differentiating to cells of the three germ lineages—endoderm, ectoderm, and mesoderm. The unstimulated iPSC controls remained undifferentiated. Phenotypic characterisation further showed a robust induction to neuronal fate with electrical stimulation, again without customary chemical inducers. Our findings add to the growing body of evidence supporting the use of electrical stimulation to augment stem cell differentiation, more specifically, pluripotent stem cell differentiation, and especially neuronal induction. Moreover, we have shown the versatility of electroactive PPy as a cell-compatible platform for advanced stem cell research and translation, including identifying novel mechanisms of fate regulation, tissue development, electroceuticals, and regenerative medicine.

Na+/H+exchanger 1 inhibition contributes to K562 leukaemic cell differentiation

2012 ◽  
Vol 36 (8) ◽  
pp. 739-745 ◽  
Author(s):  
Weina Jin ◽  
Qinghua Li ◽  
Jian Wang ◽  
Guoqiang Chang ◽  
Yani Lin ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Leukaemic Cell

scholarly journals Inducers of Friend leukaemic cell differentiation in vitro–effects of in vivo administration

1976 ◽  
Vol 33 (6) ◽  
pp. 634-645 ◽  
Author(s):  
H D Preisler ◽  
S Bjornsson ◽  
M Mori ◽  
G H Lyman
Keyword(s):  
Cell Differentiation ◽  
Leukaemic Cell ◽  
In Vitro Effects ◽  
In Vivo Administration

scholarly journals Relationship between globin mRNA accumulation and commitment to terminal cell differentiation in inducer sensitive and resistant erythroleukemia variants

Blood ◽  
1990 ◽  
Vol 76 (2) ◽  
pp. 302-306
Author(s):  
N Weich ◽  
PA Marks ◽  
RA Rifkind
Keyword(s):  
Cell Differentiation ◽  
Erythroid Differentiation ◽  
Terminal Cell ◽  
Globin Mrna ◽  
Mrna Accumulation ◽  
Chemical Inducers ◽  
Transformed Cell Line ◽  
Increased Sensitivity ◽  
Differentiation Inducers ◽  
Murine Erythroleukemia

The relationship between the kinetics of commitment to terminal cell differentiation and the rates of accumulation of globin mRNA has been examined during the induction of erythroid differentiation by polar/apolar chemical inducers in murine erythroleukemia cells (MELC), under conditions of more and less rapid commitment. Two differentiation inducers and three MELC variants have been studied. Hexamethylene bisacetamide (HMBA) initiates more rapid commitment than does dimethylsulfoxide (Me2SO). MELC variant DR10 is resistant to induction by Me2SO and responds sluggishly to HMBA, in comparison with the DS19- Sc9 variant. V3.17, an MELC variant resistant to low concentrations of vincristine, shows increased sensitivity to the inducers and an accelerated rate of commitment to terminal differentiation compared with DS19-Sc9. It is demonstrated that commitment and the actual expression of differentiation, as measured by the accumulation of alpha- , beta maj-, and beta min-globin mRNA, are temporally coordinated functions during induced differentiation of a transformed cell line by exposure to polar/apolar agents.

scholarly journals DNMT3A R882 mutants interact with polycomb proteins to block haematopoietic stem and leukaemic cell differentiation

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Junji Koya ◽  
Keisuke Kataoka ◽  
Tomohiko Sato ◽  
Masashige Bando ◽  
Yuki Kato ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Leukaemic Cell ◽  
Polycomb Proteins

Electron microscope study of the secretory activity of epithelial cells in embryonic thymus

1990 ◽  
Vol 48 (3) ◽  
pp. 382-383
Author(s):  
H. Alasam
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Epithelial Cells ◽  
Electron Density ◽  
Secretory Activity ◽  
T Cell Differentiation ◽  
High Electron ◽  
Transmission Electron ◽  
Medullary Epithelial Cells ◽  
Thymic Factor

The possibility that intrathymic T-cell differentiation involves stem cell-lymphoid interactions in embryos led us to study the ultrastructure of epithelial cell in normal embryonic thymus. Studies in adult thymus showed that it produces several peptides that induce T-cell differentiation. Several of them have been chemically characterized, such as thymosin α 1, thymopoietin, thymic humoral factor or the serum thymic factor. It was suggested that most of these factors are secreted by populations of A and B-epithelial cells.Embryonic materials were obtained from inbred matings of Swiss Albino mice. Thymuses were disected from embryos 17 days old and prepared for transmission electron microscopy. Our studies showed that embryonic thymus at this stage contains undifferentiated and differentiated epithelial cells, large lymphoblasts, medium and few small lymphocytes (Fig. 5). No differences were found between cortical and medullary epithelial cells, in contrast to the findings of Van Vliet et al,. Epithelial cells were mostly of the A-type with low electron density in both cytoplasm and nucleus. However few B-type with high electron density were also found (Fig. 7).

Role of CD5 expression on TCRγδ T cell-differentiation and development of chronic intestinal inflammation

2001 ◽  
Vol 120 (5) ◽  
pp. A517-A517
Author(s):  
A MIZOGUCHI ◽  
E MIZOGUCHI ◽  
Y DEJONG ◽  
H TAKEDATSU ◽  
F PREFFER ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Intestinal Inflammation ◽  
T Cell Differentiation ◽  
Chronic Intestinal Inflammation
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