Evidence for post-transcriptional selection of viral mRNA in cells transformed by human adenovirus 12

Nature ◽  
1976 ◽  
Vol 261 (5558) ◽  
pp. 340-342 ◽  
Author(s):  
MICHAEL R. GREEN ◽  
MAURICE GREEN ◽  
JESSE K. MACKEY
Keyword(s):  
Human Adenovirus ◽  
Viral Mrna ◽  
Selection Of ◽  
Human Adenovirus 12 ◽  
In Cells

scholarly journals Chemical Biology Framework to Illuminate Proteostasis

2020 ◽  
Vol 89 (1) ◽  
pp. 529-555 ◽  
Author(s):  
Rebecca M. Sebastian ◽  
Matthew D. Shoulders
Keyword(s):  
Quality Control ◽  
Protein Folding ◽  
Chemical Biology ◽  
Control Factors ◽  
Network Composition ◽  
Network Component ◽  
Selection Of ◽  
In Cells

Protein folding in the cell is mediated by an extensive network of >1,000 chaperones, quality control factors, and trafficking mechanisms collectively termed the proteostasis network. While the components and organization of this network are generally well established, our understanding of how protein-folding problems are identified, how the network components integrate to successfully address challenges, and what types of biophysical issues each proteostasis network component is capable of addressing remains immature. We describe a chemical biology–informed framework for studying cellular proteostasis that relies on selection of interesting protein-folding problems and precise researcher control of proteostasis network composition and activities. By combining these methods with multifaceted strategies to monitor protein folding, degradation, trafficking, and aggregation in cells, researchers continue to rapidly generate new insights into cellular proteostasis.

scholarly journals A transcriptional enhancer and an interferon-responsive sequence in major histocompatibility complex class I genes.

1986 ◽  
Vol 6 (10) ◽  
pp. 3550-3554 ◽  
Author(s):  
J Vogel ◽  
M Kress ◽  
G Khoury ◽  
G Jay
Keyword(s):  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
Human Adenovirus ◽  
Class I ◽  
Complex Class ◽  
Transcriptional Enhancer ◽  
Histocompatibility Complex ◽  
Flanking Region ◽  
Cat Gene ◽  
Human Adenovirus 12

The major histocompatibility complex class I antigens play an indispensable role in cell-cell interactions. Perturbation of their expression has been shown to have deleterious physiological consequences, including the escape of transformed cells from immune detection. In an attempt to understand how class I genes are regulated, we dissected the Ld gene to identify potential control regions. By using a test vector containing the simian virus 40 early promoter placed upstream of the bacterial chloramphenicol acetyltransferase (cat) gene, we demonstrated the presence of a transcriptional enhancer within the 5'-flanking region. The sequence is functional in both orientations and has been mapped within 350 base pairs upstream of the Ld transcriptional start site. Although human adenovirus 12 can suppress endogenous class I genes, it cannot down-regulate the activity of the transiently transfected cat gene which has been placed under the control of the Ld enhancer and promoter. Our results suggested that if the human adenovirus 12-induced function regulates the expression of class I genes by a trans mechanism, then its target site must not be within 1.9 kilobases of the 5'-flanking region. Treatment of cells with interferon increases the accumulation of class I transcripts. Expression of the cat gene under the control of the Ld enhancer and promoter also can be up-regulated by interferon. Our study shows that the target sequence required for this enhancement resides, at least in part, within the same 350-base pair segment which contains the transcriptional enhancer.

Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes

2006 ◽  
Vol 31 (1) ◽  
pp. 78-86 ◽  
Author(s):  
M Rathod ◽  
S D Vangipuram ◽  
B Krishnan ◽  
A R Heydari ◽  
T C Holland ◽  
...  
Keyword(s):  
Dna Replication ◽  
Mrna Expression ◽  
Human Adenovirus ◽  
Viral Mrna

121 HUMAN ADENOVIRUS 12-INDUCED RETINAL NEOPLASM IN OLIVE BABOON

1980 ◽  
Vol 39 (3) ◽  
pp. 377
Author(s):  
N. Mukai ◽  
S. Kalter ◽  
B. Cummins ◽  
V. Matthews ◽  
T. Nakajima ◽  
...  
Keyword(s):  
Human Adenovirus ◽  
Olive Baboon ◽  
Human Adenovirus 12

scholarly journals Multiple Copies of Human Adenovirus 12 Genomes Are Integrated in Virus-Induced Hamster Tumors

1977 ◽  
Vol 22 (1) ◽  
pp. 238-242 ◽  
Author(s):  
Michael R. Green ◽  
Jesse K. Mackey ◽  
Maurice Green
Keyword(s):  
Human Adenovirus ◽  
Multiple Copies ◽  
Human Adenovirus 12

A transcriptional enhancer and an interferon-responsive sequence in major histocompatibility complex class I genes

1986 ◽  
Vol 6 (10) ◽  
pp. 3550-3554
Author(s):  
J Vogel ◽  
M Kress ◽  
G Khoury ◽  
G Jay
Keyword(s):  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
Human Adenovirus ◽  
Class I ◽  
Complex Class ◽  
Transcriptional Enhancer ◽  
Histocompatibility Complex ◽  
Flanking Region ◽  
Cat Gene ◽  
Human Adenovirus 12

The major histocompatibility complex class I antigens play an indispensable role in cell-cell interactions. Perturbation of their expression has been shown to have deleterious physiological consequences, including the escape of transformed cells from immune detection. In an attempt to understand how class I genes are regulated, we dissected the Ld gene to identify potential control regions. By using a test vector containing the simian virus 40 early promoter placed upstream of the bacterial chloramphenicol acetyltransferase (cat) gene, we demonstrated the presence of a transcriptional enhancer within the 5'-flanking region. The sequence is functional in both orientations and has been mapped within 350 base pairs upstream of the Ld transcriptional start site. Although human adenovirus 12 can suppress endogenous class I genes, it cannot down-regulate the activity of the transiently transfected cat gene which has been placed under the control of the Ld enhancer and promoter. Our results suggested that if the human adenovirus 12-induced function regulates the expression of class I genes by a trans mechanism, then its target site must not be within 1.9 kilobases of the 5'-flanking region. Treatment of cells with interferon increases the accumulation of class I transcripts. Expression of the cat gene under the control of the Ld enhancer and promoter also can be up-regulated by interferon. Our study shows that the target sequence required for this enhancement resides, at least in part, within the same 350-base pair segment which contains the transcriptional enhancer.

Sign in / Sign up

Export Citation Format

Share Document