In vivo localisation of radiolabelled antibodies to carcinoembryonic antigen in human colon carcinoma grafted into nude mice

Nature ◽  
1974 ◽  
Vol 248 (5450) ◽  
pp. 704-706 ◽  
Author(s):  
J.-P. MACH ◽  
S. CARREL ◽  
C. MERENDA ◽  
B. SORDAT ◽  
J.-C. CEROTTINI
Keyword(s):  
Carcinoembryonic Antigen ◽  
Colon Carcinoma ◽  
Nude Mice ◽  
Human Colon ◽  
Human Colon Carcinoma ◽  
Radiolabelled Antibodies

scholarly journals Radiolabeled fragments of monoclonal antibodies against carcinoembryonic antigen for localization of human colon carcinoma grafted into nude mice.

1983 ◽  
Vol 158 (2) ◽  
pp. 413-427 ◽  
Author(s):  
F Buchegger ◽  
C M Haskell ◽  
M Schreyer ◽  
B R Scazziga ◽  
S Randin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Carcinoembryonic Antigen ◽  
Colon Carcinoma ◽  
Direct Measurement ◽  
Nude Mice ◽  
Human Colon ◽  
Whole Body ◽  
Fab Fragments ◽  
Human Colon Carcinoma ◽  
Apical Side

Four monoclonal antibodies against carcinoembryonic antigen (CEA) have been selected from 32 hybrids that produce antibodies against this antigen, by the criteria of high affinity for CEA and low cross-reactivity with granulocyte glycoprotein(s). The specificity of tumor localization in vivo of the four MAb, and their F(ab')2 and Fab fragments was compared in nude mice bearing grafts of a serially transplanted, CEA-producing, human colon carcinoma. The distribution of radiolabeled MAb and their fragments after intravenous injection was analyzed by direct measurement of radioactivity in tumor and normal organs, as well as by whole-body scanning and by autoradiography of tumor sections. Paired labeling experiments, in which 131I-labeled antibody or fragments and 125I-labeled control IgG are injected simultaneously, were undertaken to determine the relative tumor uptakes of each labeled protein. The tumor antibody uptake divided by that of control IgG defines the specificity index of localization. Tumor antibody uptakes (as compared with the whole mouse), ranging between 7 and 15, and specificity indices ranging between 3.4 and 6.8, were obtained with the four intact MAb at day 4-5 after injection. With F(ab')2 fragments of the four MAb, at day 3, the tumor antibody uptakes ranged between 12 and 24 and the specificity indices between 5.3 and 8.2. With the Fab fragments prepared from the two most promising MAb, the antibody uptakes reached values of 34 and 82 at day 2-3 and the specificity indices were as high as 12 and 19. The scanning results paralleled those obtained by direct measurement of radioactivity. With intact MAb, tumor grafts of 0.5-1 g gave very contrasted positive scans 3 d after injection. Using MAb fragments, tumors of smaller size were detectable earlier. The best results were obtained with Fab fragments of MAb 35, which gave clear detections of tumors weighing only 0.1 g as early as 48 h after injection. Autoradiographs of tumor sections from mice injected with 125I-labeled MAb demonstrated that the radioactivity was localized in the tumor tissues and not in the stromal connective tissue of mouse origin. The highest radioactivity concentration was localized in areas known to contain CEA such as the pseudolumen of glands and the apical side of carcinoma cells. The penetration of radioactivity in the central part of tumor nodules and the pseudolumen appeared to be increased with the use of MAb fragments.

scholarly journals Ablation of human colon carcinoma in nude mice by 131I-labeled monoclonal anti-carcinoembryonic antigen antibody F(ab')2 fragments.

1989 ◽  
Vol 83 (5) ◽  
pp. 1449-1456 ◽  
Author(s):  
F Buchegger ◽  
C Pfister ◽  
K Fournier ◽  
F Prevel ◽  
M Schreyer ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Colon Carcinoma ◽  
Nude Mice ◽  
Human Colon ◽  
Human Colon Carcinoma ◽  
Antigen Antibody

Sensitizing human colon carcinoma HT-29 cells to cisplatin by cyclopentenylcytosine, in vitro and in vivo

Life Sciences ◽  
2000 ◽  
Vol 68 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Kamran Gharehbaghi ◽  
Thomas Szekeres ◽  
Joel A. Yalowitz ◽  
Monika Fritzer-Szekeres ◽  
Yves G. Pommier ◽  
...  
Keyword(s):  
Colon Carcinoma ◽  
Human Colon ◽  
Human Colon Carcinoma ◽  
Ht 29

scholarly journals TGF-beta 1 is an autocrine-negative growth regulator of human colon carcinoma FET cells in vivo as revealed by transfection of an antisense expression vector.

1992 ◽  
Vol 116 (1) ◽  
pp. 187-196 ◽  
Author(s):  
S P Wu ◽  
D Theodorescu ◽  
R S Kerbel ◽  
J K Willson ◽  
K M Mulder ◽  
...  
Keyword(s):  
Cell Growth ◽  
Colon Carcinoma ◽  
Growth Regulator ◽  
Human Colon ◽  
Tgf Beta ◽  
Transfected Cells ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma

Transforming growth factor-beta 1 (TGF-beta 1) has previously been implicated as a potential negative autocrine or paracrine growth regulator of certain cell types (Arteaga, C. L., R. J. Coffey, Jr., T. C. Dugger, C. M. McCutchen, H. L. Moses, and R. M. Lyons. 1990. Cell Growth & Differ. 1:367-374; Hafez, M. M., D. Infante, S. Winawer, and E. Friedman. 1990. Cell Growth & Differ. 1:617-626; Glick, A. B., K. C. Flanders, D. Danielpour, S. H. Yuspa, and M. B. Sporn. 1989. Cell Regulation. 1:87-97). This is based mainly on experiments assessing the effects of exogenous TGF-beta 1 or neutralizing antibodies to TGF-beta 1 on normal or tumor cell proliferation in vitro. However, direct evidence demonstrating such a negative regulation of tumor cell growth in vivo is still lacking. To overcome this problem we have constructed and used an antisense expression vector for TGF-beta 1 as a means of regulating endogenous TGF-beta 1 expression in tumor cells. Antisense-transfected FET human colon carcinoma cells showed a fivefold reduction in TGF-beta 1 mRNA and 15-fold reduction in TGF-beta 1 secretion. Antisense mRNA was detected in transfected cells by an RNase protection assay. Compared to control cells, cultured antisense-transfected cells showed a reduction in lag phase time rather than a change in doubling time. Cloning efficiencies of transfected cells were four times greater than control cells in anchorage-independent assays. Control cells did not form tumors at 5 x 10(5) in athymic nude mice. Antisense-transfected cells formed tumors in 40% of animals injected. At higher inocula (1 x 10(6) cells) antisense-transfected cells formed tumors in 100% of animals injected, but control cells still failed to form tumors. These results show that TGF-beta 1 acts as a negative growth regulator of human colon carcinoma cells in vivo as well as in vitro. Acquisition of partial or full resistance to such inhibitory effects may therefore contribute to tumor development and progression.

scholarly journals Comparison of Seven Iodine-Labelled Monoclonal Antibodies in Nude Mice with Human Colon Carcinoma Xenografts

1991 ◽  
Vol 30 (3) ◽  
pp. 385-393 ◽  
Author(s):  
E. Forssell Aronsson ◽  
J. Grétarsdóttir ◽  
L. Jacobsson ◽  
S. Mattsson ◽  
S. B. Holmberg ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Colon Carcinoma ◽  
Nude Mice ◽  
Human Colon ◽  
Human Colon Carcinoma
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