The induction of tumour immunity in mice using glutaraldehyde-treated tumour cells

Nature ◽  
1974 ◽  
Vol 248 (5450) ◽  
pp. 690-691 ◽  
Author(s):  
C. J. SANDERSON ◽  
P. FROST
Keyword(s):  
Tumour Cells ◽  
Tumour Immunity ◽  
Treated Tumour

scholarly journals Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells

Cells ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 49 ◽  
Author(s):  
Serena Saladini ◽  
Michele Aventaggiato ◽  
Federica Barreca ◽  
Emanuela Morgante ◽  
Luigi Sansone ◽  
...  
Keyword(s):  
Apoptotic Cell ◽  
Tumour Cells ◽  
Glutamine Metabolism ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Ammonia Accumulation ◽  
Tumour Cell Lines ◽  
Type 2 Diabetic ◽  
Treated Tumour

Metformin has been shown to inhibit glutaminase (GLS) activity and ammonia accumulation thereby reducing the risk of hepatic encephalopathy in type 2 diabetic patients. Since tumour cells are addicted to glutamine and often show an overexpression of glutaminase, we hypothesize that the antitumoral mechanism of metformin could be ascribed to inhibition of GLS and reduction of ammonia and ammonia-induced autophagy. Our results show that, in different tumour cell lines, micromolar doses of metformin prevent cell growth by reducing glutamate, ammonia accumulation, autophagy markers such as MAP1LC3B-II and GABARAP as well as degradation of long-lived proteins. Reduced autophagy is then accompanied by increased BECN1/BCL2 binding and apoptotic cell death. Interestingly, GLS-silenced cells reproduce the effect of metformin treatment showing reduced MAP1LC3B-II and GABARAP as well as ammonia accumulation. Since metformin is used as adjuvant drug to increase the efficacy of Cisplatin-based neoadjuvant chemotherapy, we co-treated tumour cells with micromolar doses of metformin in the presence of cisplatin observing a marked reduction of MAP1LC3B-II and an increase of caspase 3 cleavage. In conclusion, our work demonstrates that the anti-tumoral action of metformin is due to the inhibition of glutaminase and autophagy and could be used to improve the efficacy of chemotherapy.

The Effects of Ultrasound and Light on Indocyanine-Green-Treated Tumour Cells and Tissues

ChemMedChem ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. 1465-1471 ◽  
Author(s):  
Nikolitsa Nomikou ◽  
Christine Sterrett ◽  
Ciara Arthur ◽  
Bridgeen McCaughan ◽  
John F. Callan ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Tumour Cells ◽  
Treated Tumour

Skin tests with autologous cholesteryl hemisuccinate treated tumour cells in cancer patients

1985 ◽  
Vol 20 (2) ◽  
pp. 179-181 ◽  
Author(s):  
Marta Munzarová ◽  
Daniela Zemanová ◽  
Jan Kovařik ◽  
Aleš Rejthar
Keyword(s):  
Cancer Patients ◽  
Tumour Cells ◽  
Skin Tests ◽  
Cholesteryl Hemisuccinate ◽  
Treated Tumour

scholarly journals Evaluation of a canine transmissible venereal tumour cell line with tumour immunity capacity but without tumorigenic property

2019 ◽  
Vol 63 (2) ◽  
pp. 225-233 ◽  
Author(s):  
Yareellys Ramos Zayas ◽  
Moisés Armides Franco Molina ◽  
Reyes Tamez Guerra ◽  
Cristina Rodríguez Padilla
Keyword(s):  
Cell Line ◽  
Financial Burden ◽  
Morphological Characteristics ◽  
Tumour Cells ◽  
Tumour Cell Line ◽  
Histopathological Analysis ◽  
Isolated Cells ◽  
Tumour Immunity ◽  
Canine Transmissible Venereal Tumour ◽  
Transmissible Venereal Tumour

AbstractIntroduction:Canine transmissible venereal tumour (CTVT) is a sexually transmitted tumour affecting dogs worldwide, imposing a financial burden on dog owners. A stable culture cell line in continuous passages for >18 months has only been achieved once. The present study investigated a stable CTVT cell line isolated from a bitch and its potential as a vaccine.Material and Methods:A biopsy from a 2-year-old mongrel bitch with CTVT was obtained for histopathological confirmation and isolation of tumour cells. The isolated cells were cultured to passage 55 and characterised by flow cytometry, with karyotyping by GTG-banding and by PCR detection of myc S-2 and LINE AS1. The isolated CTVT cell line was also used as a preventive vaccine in a canine model.Results:Histopathological analysis of the isolated tumour cells revealed typical CTVT characteristics. Constant proliferation and stable morphological characteristics were observed during culture. Phenotypic analysis determined the expression of HLA-DR+, CD5.1+, CD14+, CD45+, CD83+, CD163+, and Ly-6G-Ly-6C+. GTG-banding revealed a mean of 57 chromosomes in the karyotype with several complex chromosomal rearrangements. LINE-c-myc insertion in the isolated CTVT cell line at 550 bp was not detected. However, a 340-bp band was amplified. Isolated CTVT cell line inoculation at a concentration of 1×108did not induce tumour growth in bitches, nor did a challenge with primary CTVT cells.Conclusion:The present study successfully identified and isolated a stable CTVT cell line that may be useful in CTVT prevention.

Tumour Immunity induced in Mice with Cell-free Homogenates of Influenza Virus-infected Tumour Cells

Nature ◽  
1971 ◽  
Vol 231 (5300) ◽  
pp. 265-266 ◽  
Author(s):  
C. BOONE ◽  
K. BLACKMAN ◽  
P. BRANDCHAFT
Keyword(s):  
Influenza Virus ◽  
Tumour Cells ◽  
Tumour Immunity

Heated tumour cells of autologous and allogeneic origin elicit anti-tumour immunity

2004 ◽  
Vol 53 (4) ◽  
pp. 323-330 ◽  
Author(s):  
Diane Soars ◽  
Angus G. Dalgleish ◽  
Alan A. Melcher ◽  
Hardev S. Pandha ◽  
Stephen M. Todryk ◽  
...  
Keyword(s):  
Tumour Cells ◽  
Tumour Immunity
Sign in / Sign up

Export Citation Format

Share Document