In vitro assembly of 30S ribosomal particles from precursor 16S RNA of Escherichia coli

Nature ◽  
1974 ◽  
Vol 247 (5442) ◽  
pp. 552-554 ◽  
Author(s):  
JOHN W. WIREMAN ◽  
PAUL S. SYPHERD
Keyword(s):  
Escherichia Coli ◽  
16S Rna ◽  
In Vitro Assembly

Conversion of Escherichia coli 17S chloramphenicol RNA to a 16S RNA in vitro

Biochimie ◽  
1976 ◽  
Vol 58 (8) ◽  
pp. 1007-1009 ◽  
Author(s):  
Jacques Alain Tréton ◽  
Françoise Hayes
Keyword(s):  
Escherichia Coli ◽  
16S Rna

In vitro assembly of 30S and 70S bacterial ribosomes from 16S RNA containing single base substitutions, insertions, and deletions around the decoding site (C1400)

Biochemistry ◽  
1989 ◽  
Vol 28 (3) ◽  
pp. 1002-1011 ◽  
Author(s):  
Robert Denman ◽  
Carl Weitzmann ◽  
Philip R. Cunningham ◽  
Didier Negre ◽  
Kelvin Nurse ◽  
...  
Keyword(s):  
Single Base ◽  
Insertions And Deletions ◽  
Base Substitutions ◽  
16S Rna ◽  
In Vitro Assembly

scholarly journals In Vitro Assembly of Cowpea Chlorotic Mottle Virus from Coat Protein Expressed in Escherichia coli and in Vitro-Transcribed Viral cDNA

Virology ◽  
1995 ◽  
Vol 207 (2) ◽  
pp. 486-494 ◽  
Author(s):  
Xiaoxia Zhao ◽  
James M. Fox ◽  
Norman H. Olson ◽  
Timothy S. Baker ◽  
Mark J. Young
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
In Vitro Assembly ◽  
Viral Cdna

scholarly journals In vitro assembly of a prepriming complex at the origin of the Escherichia coli chromosome.

1987 ◽  
Vol 262 (21) ◽  
pp. 10327-10334 ◽  
Author(s):  
B.E. Funnell ◽  
T.A. Baker ◽  
A. Kornberg
Keyword(s):  
Escherichia Coli ◽  
In Vitro Assembly

scholarly journals In Vitro Assembly of Alphavirus Cores by Using Nucleocapsid Protein Expressed in Escherichia coli

1999 ◽  
Vol 73 (7) ◽  
pp. 5309-5319 ◽  
Author(s):  
Timothy L. Tellinghuisen ◽  
Agnes E. Hamburger ◽  
Bonnie R. Fisher ◽  
Ralf Ostendorp ◽  
Richard J. Kuhn
Keyword(s):  
Escherichia Coli ◽  
Capsid Protein ◽  
Sindbis Virus ◽  
Expression System ◽  
Molar Ratio ◽  
Ross River Virus ◽  
Nucleocapsid Proteins ◽  
In Vitro Assembly ◽  
Amino Acid Requirements

ABSTRACT The production of the alphavirus virion is a multistep event requiring the assembly of the nucleocapsid core in the cytoplasm and the maturation of the glycoproteins in the endoplasmic reticulum and the Golgi apparatus. These components associate during the budding process to produce the mature virion. The nucleocapsid proteins of Sindbis virus and Ross River virus have been produced in a T7-basedEscherichia coli expression system and purified. In the presence of single-stranded but not double-stranded nucleic acid, the proteins oligomerize in vitro into core-like particles which resemble the native viral nucleocapsid cores. Despite their similarities, Sindbis virus and Ross River virus capsid proteins do not form mixed core-like particles. Truncated forms of the Sindbis capsid protein were used to establish amino acid requirements for assembly. A capsid protein starting at residue 19 [CP(19–264)] was fully competent for in vitro assembly, whereas proteins with further N-terminal truncations could not support assembly. However, a capsid protein starting at residue 32 or 81 was able to incorporate into particles in the presence of CP(19–264) or could inhibit assembly if its molar ratio relative to CP(19–264) was greater than 1:1. This system provides a basis for the molecular dissection of alphavirus core assembly.

The absence of modified nucleotides affects both in vitro assembly and in vitro function of the 30S ribosomal subunit of Escherichia coli

Biochimie ◽  
1991 ◽  
Vol 73 (6) ◽  
pp. 789-796 ◽  
Author(s):  
P.R. Cunningham ◽  
R.B. Richard ◽  
C.J. Weitzmann ◽  
K. Nurse ◽  
J. Ofengand
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Subunit ◽  
Modified Nucleotides ◽  
In Vitro Assembly
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