Production of Antibody to Sheep Red Blood Cells by Human Tonsil Cells in vitro

Nature ◽  
1973 ◽  
Vol 243 (5407) ◽  
pp. 408-410 ◽  
Author(s):  
MICHAEL K. HOFFMANN ◽  
DELF SCHMIDT ◽  
HERBERT F. OETTGEN
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Tonsil ◽  
Sheep Red Blood Cells

scholarly journals In vitro antioxidant effects of Trigonella foenum graecum (L.) Fenugreek seed extract on sheep red blood cells

2012 ◽  
Vol 6 (38) ◽  
pp. 5119-5127 ◽  
Author(s):  
Dinakaran Vasudevan ◽  
Sridharan Subhashree ◽  
Periyasamy Latha ◽  
Sudha Rani Sankaramoorthy
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Seed Extract ◽  
Trigonella Foenum Graecum ◽  
Fenugreek Seed ◽  
Sheep Red Blood Cells ◽  
Trigonella Foenum Graecum L ◽  
Antioxidant Effects

Differentiation of Na(+)-K+ pumps of low-K+ sheep red blood cells is promoted by Lp membrane antigens

1993 ◽  
Vol 265 (1) ◽  
pp. C99-C105 ◽  
Author(s):  
Z. C. Xu ◽  
P. B. Dunham ◽  
B. Dyer ◽  
R. Blostein
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Rat Kidney ◽  
Striking Increase ◽  
Sheep Red Blood Cells ◽  
High K ◽  
Membrane Antigens ◽  
Low K ◽  
Kinetics Of

Na(+)-K+ pumps of red blood cells from sheep of the low-K+ (LK) phenotype undergo differentiation during circulation, manifested in part by a striking increase in sensitivity to inhibition by intracellular K+ (Ki). Pumps of red blood cells from sheep from the allelic phenotype, high K+ (HK), do not undergo this type of maturation. The hypothesis was tested that the Lp antigen, found on LK but not HK cells, is responsible for the maturation of LK pumps. Lp antigens have been shown to inhibit LK pumps because anti-Lp antibody stimulates the pumps by relieving inhibition by the antigen. Lp antigens were recently shown to be molecular entities separate from Na(+)-K+ pumps [Xu, Z.-C., P. Dunham, J. Munzer, J. Silvius, and R. Blostein. Am. J. Physiol. 263 (Cell Physiol. 32): C1007-C1014, 1992]. The test of the hypothesis was to modify the Lp antigens of immature LK red blood cells with two kinds of treatments, anti-Lp antibody and trypsinization (which cleaves Lp), and to observe the effects of these treatments on maturation of pumps during culture of the cells in vitro. Both of these treatments prevented the maturation of the kinetics of the pumps to the Ki-sensitive pattern, supporting the hypothesis that interaction of the pumps with Lp antigens is responsible for the maturation of the pumps. Strong supportive evidence came from experiments on Na(+)-K+ pumps from rat kidney delivered into immature LK sheep red blood cells by microsome fusion.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Two Distinct Hemolytic Activities inXenorhabdus nematophila Are Active against Immunocompetent Insect Cells

2001 ◽  
Vol 67 (6) ◽  
pp. 2515-2525 ◽  
Author(s):  
Julien Brillard ◽  
Carlos Ribeiro ◽  
Noël Boemare ◽  
Michel Brehélin ◽  
Alain Givaudan
Keyword(s):  
Stationary Phase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Cytolytic Activity ◽  
Bacterial Cells ◽  
Xenorhabdus Nematophila ◽  
Sheep Red Blood Cells ◽  
Insertional Inactivation ◽  
Wild Strains

ABSTRACT Xenorhabdus spp. and Photorhabdus spp. are major insect bacterial pathogens symbiotically associated with nematodes. These bacteria are transported by their nematode hosts into the hemocoel of the insect prey, where they proliferate within hemolymph. In this work we report that wild strains belonging to different species of both genera are able to produce hemolysin activity on blood agar plates. Using a hemocyte monolayer bioassay, cytolytic activity against immunocompetent cells from the hemolymph ofSpodoptera littoralis (Lepidoptera: Noctuidae) was found only in supernatants of Xenorhabdus; none was detected in supernatants of various strains of Photorhabdus. During in vitro bacterial growth of Xenorhabdus nematophila F1, two successive bursts of cytolytic activity were detected. The first extracellular cytolytic activity occurred when bacterial cells reached the stationary phase. It also displayed a hemolytic activity on sheep red blood cells, and it was heat labile. Among insect hemocyte types, granulocytes were the preferred target. Lysis of hemocytes by necrosis was preceded by a dramatic vacuolization of the cells. In contrast the second burst of cytolytic activity occurred late during stationary phase and caused hemolysis of rabbit red blood cells, and insect plasmatocytes were the preferred target. This second activity is heat resistant and produced shrinkage and necrosis of hemocytes. Insertional inactivation of flhD gene in X. nematophilaleads to the loss of hemolysis activity on sheep red blood cells and an attenuated virulence phenotype in S. littoralis (A. Givaudan and A. Lanois, J. Bacteriol. 182:107–115, 2000). This mutant was unable to produce the early cytolytic activity, but it always displayed the late cytolytic effect, preferably active on plasmatocytes. Thus, X. nematophila produced two independent cytolytic activities against different insect cell targets known for their major role in cellular immunity.

Modulation of the secondary antibody response of murine lymphocytes to sheep red blood cells in vitro by neuraminidase and exoglycosidases

1991 ◽  
Vol 29 (3) ◽  
pp. 235-240 ◽  
Author(s):  
Makoto Nakamura ◽  
Tomoaki Yoshida ◽  
Ken-ichi Isobe ◽  
Takashi Iwamoto ◽  
S.M. Jamshedur Rahman ◽  
...  
Keyword(s):  
Antibody Response ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Secondary Antibody ◽  
Sheep Red Blood Cells

The Effect of Zinc and Other Metals on Complement Hemolysis of Sheep Red Blood Cells in Vitro

1974 ◽  
Vol 145 (1) ◽  
pp. 263-267 ◽  
Author(s):  
D. W. Montgomery ◽  
L. K. Don ◽  
C. F. Zukoski ◽  
M. Chvapil
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Sheep Red Blood Cells
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