The Regulation of Intestinal Calcium Transport by Vitamin D

Nature ◽  
1973 ◽  
Vol 243 (5402) ◽  
pp. 89-91 ◽  
Author(s):  
G. PATRICK
Keyword(s):  
Vitamin D ◽  
Calcium Transport ◽  
Intestinal Calcium Transport

Mechanism of regulation of calcium-pumping activity in chick intestine

1992 ◽  
Vol 262 (5) ◽  
pp. G797-G805
Author(s):  
J. Takito ◽  
T. Shinki ◽  
H. Tanaka ◽  
T. Suda
Keyword(s):  
Vitamin D ◽  
Calcium Transport ◽  
Calcium Uptake ◽  
Early Stage ◽  
Basolateral Membrane ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Membrane Vesicles ◽  
Intestinal Calcium Transport ◽  
Pumping Activity

The role of the calcium pump in the stimulation of intestinal calcium transport activity by 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3] was examined in chicks. The in situ intestinal absorption of calcium increased approximately threefold in the duodenum, jejunum, and ileum 6 h after a single injection of 625 ng of 1 alpha,25(OH)2D3 into vitamin D-deficient chicks. The same treatment also increased approximately twofold the rate of ATP-dependent calcium uptake by the basolateral membrane vesicles (BL) isolated from those three sites. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that a Mg(2+)-dependent calcium-stimulated phosphorylated intermediate with an apparent molecular mass of 105 kDa appeared in the BL. The 1 alpha,25(OH)2D3 treatment gave no change in the levels of the intermediate. Pretreatment of the BL with alkaline phosphatase decreased the calcium uptake by the BL isolated from 1 alpha,25(OH)2D3-treated chicks, but it had little effect on the uptake by the BL from vitamin D-deficient chicks. These results suggest that at an early stage of the 1 alpha,25(OH)2D3-induced intestinal calcium transport process, the vitamin regulates the calcium-pumping activity of chick intestinal BL by phosphorylation and dephosphorylation but not by a stoichiometric change in the pump.

scholarly journals Evidence for a Role of Prolactin in Calcium Homeostasis: Regulation of Intestinal Transient Receptor Potential Vanilloid Type 6, Intestinal Calcium Absorption, and the 25-Hydroxyvitamin D3 1α Hydroxylase Gene by Prolactin

Endocrinology ◽  
2010 ◽  
Vol 151 (7) ◽  
pp. 2974-2984 ◽  
Author(s):  
Dare V. Ajibade ◽  
Puneet Dhawan ◽  
Adam J. Fechner ◽  
Mark B. Meyer ◽  
J. Wesley Pike ◽  
...  
Keyword(s):  
Vitamin D ◽  
Calcium Homeostasis ◽  
Calcium Transport ◽  
Transient Receptor Potential ◽  
Prolactin Receptor ◽  
Receptor Potential ◽  
Transient Receptor Potential Vanilloid ◽  
Calbindin D9k ◽  
Intestinal Calcium Transport ◽  
Transient Receptor

Increased calcium transport has been observed in vitamin D-deficient pregnant and lactating rats, indicating that another factor besides 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) is involved in intestinal calcium transport. To investigate prolactin as a hormone involved in calcium homeostasis, vitamin D-deficient male mice were injected with 1,25(OH)2D3, prolactin, or prolactin + 1,25(OH)2D3. Prolactin alone (1 μg/g body weight 48, 24, and 4 h before termination) significantly induced duodenal transient receptor potential vanilloid type 6 (TRPV6) mRNA (4-fold) but caused no change in calbindin-D9k. Combined treatment with 1,25(OH)2D3 and prolactin resulted in an enhancement of the 1,25(OH)2D3 induction of duodenal TRPV6 mRNA, calbindin-D9k mRNA, and an induction of duodenal calcium transport [P < 0.05 compared with 1,25(OH)2D3 alone]. Because lactation is associated with an increase in circulating 1,25(OH)2D3, experiments were done to determine whether prolactin also has a direct effect on induction of 25-hydroxyvitamin D3 1α hydroxylase [1α(OH)ase]. Using AOK B-50 cells cotransfected with the prolactin receptor and the mouse 1α(OH)ase promoter −1651/+22 cooperative effects between prolactin and signal transducer and activator of transcription 5 were observed in the regulation of 1α(OH)ase. In addition, in prolactin receptor transfected AOK B-50 cells, prolactin treatment (400 ng/ml) and signal transducer and activator of transcription 5 significantly induced 1α(OH)ase protein as determined by Western blot analysis. Thus, prolactin, by multiple mechanisms, including regulation of vitamin D metabolism, induction of TRPV6 mRNA, and cooperation with 1,25(OH)2D3 in induction of intestinal calcium transport genes and intestinal calcium transport, can act as an important modulator of vitamin D-regulated calcium homeostasis.

Vitamin D and Intestinal Calcium Transport: Facts, Speculations and Hypotheses

1995 ◽  
Vol 125 (suppl_7) ◽  
pp. 1971S-1979S ◽  
Author(s):  
Robert H. Wasserman ◽  
Curtis S. Fullmer
Keyword(s):  
Vitamin D ◽  
Calcium Transport ◽  
Intestinal Calcium Transport

Vitamin D dependence and aboral gradient ofin vivo intestinal calcium transport in the rat

1978 ◽  
Vol 23 (10) ◽  
pp. 943-947 ◽  
Author(s):  
M. Michael Petith ◽  
Janice R. Wenger ◽  
H. P. Schedl
Keyword(s):  
Vitamin D ◽  
Calcium Transport ◽  
Intestinal Calcium Transport
Sign in / Sign up

Export Citation Format

Share Document