A Technique for Studying Sperm Maturation in vitro

Nature ◽  
1972 ◽  
Vol 239 (5369) ◽  
pp. 227-228 ◽  
Author(s):  
M. C. ORGEBIN-CRIST ◽  
P. L. TICHENOR
Keyword(s):  
Sperm Maturation ◽  
Maturation In Vitro

Effect of Testosterone on Sperm Maturation in vitro

Nature ◽  
1973 ◽  
Vol 245 (5424) ◽  
pp. 328-329 ◽  
Author(s):  
M. C. ORGEBIN-CRIST ◽  
P. L. TICHENOR
Keyword(s):  
Sperm Maturation ◽  
Maturation In Vitro

scholarly journals Induction of sperm maturation in vitro in epididymal cell cultures of the tammar wallaby (Macropus eugenii): disruption of motility initiation and sperm morphogenesis by inhibition of actin polymerization

Reproduction ◽  
2002 ◽  
pp. 107-117 ◽  
Author(s):  
M Lin ◽  
R Hess ◽  
RJ Aitken
Keyword(s):  
Culture System ◽  
Actin Polymerization ◽  
Tammar Wallaby ◽  
Sperm Maturation ◽  
Actin Organization ◽  
Cell Monolayers ◽  
Macropus Eugenii ◽  
Maturation In Vitro ◽  
Marsupial Species

A sperm-epididymal cell co-culture was shown to be capable of inducing the in vitro maturation of spermatozoa from a marsupial species, the tammar wallaby (Macropus eugenii). This system was able to maintain wallaby epididymal epithelial cells in vitro for more than 2 months. The system also enabled immature wallaby spermatozoa to differentiate from a T-shaped to a streamlined form, accompanied by the development of progressive motility after co-culture with epididymal cell monolayers that had been cultured for 7 days. The addition of inhibitors of actin polymerization (latrunculin A or B) to the co-culture system showed that wallaby sperm maturation was impaired by the interruption of actin organization within the immature spermatozoa. These results indicate that actin filaments play a significant role in sperm transformation during post-testicular maturation in marsupials. These observations also indicate that the marsupial co-culture system has the potential to greatly increase understanding of sperm-epididymal cell interactions and the mechanism of sperm maturation in these species.

scholarly journals Caput Ligation Renders Immature Mouse Sperm Motile and Capable to Undergo cAMP-Dependent Phosphorylation

2021 ◽  
Vol 22 (19) ◽  
pp. 10241
Author(s):  
Darya A. Tourzani ◽  
Maria A. Battistone ◽  
Ana M. Salicioni ◽  
Sylvie Breton ◽  
Pablo E. Visconti ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Female Reproductive Tract ◽  
Sperm Maturation ◽  
Molecular Pathways ◽  
Epididymal Sperm ◽  
Mammalian Sperm ◽  
Maturation In Vitro ◽  
Epididymal Maturation ◽  
Immature Mouse

Mammalian sperm must undergo two post-testicular processes to become fertilization-competent: maturation in the male epididymis and capacitation in the female reproductive tract. While caput epididymal sperm are unable to move and have not yet acquired fertilization potential, sperm in the cauda epididymis have completed their maturation, can move actively, and have gained the ability to undergo capacitation in the female tract or in vitro. Due to the impossibility of mimicking sperm maturation in vitro, the molecular pathways underlying this process remain largely unknown. We aimed to investigate the use of caput epididymal ligation as a tool for the study of sperm maturation in mice. Our results indicate that after seven days of ligation, caput sperm gained motility and underwent molecular changes comparable with those observed for cauda mature sperm. Moreover, ligated caput sperm were able to activate pathways related to sperm capacitation. Despite these changes, ligated caput sperm were unable to fertilize in vitro. Our results suggest that transit through the epididymis is not required for the acquisition of motility and some capacitation-associated signaling but is essential for full epididymal maturation. Caput epididymal ligation is a useful tool for the study of the molecular pathways involved in the acquisition of sperm motility during maturation.

scholarly journals Glycine ameliorates mitochondrial dysfunction caused by ABT-199 in porcine oocytes

2021 ◽  
Author(s):  
Sicong Yu ◽  
Lepeng Gao ◽  
Yang Song ◽  
Xin Ma ◽  
Shuang Liang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dysfunction ◽  
Oocyte Maturation ◽  
Mitochondrial Function ◽  
Protective Action ◽  
Damage Model ◽  
Developmental Competence ◽  
Free Calcium ◽  
Maturation In Vitro

Abstract Mitochondria play an important role in controlling oocyte developmental competence. Our previous studies showed that glycine can regulate mitochondrial function and improve oocyte maturation in vitro. However, the mechanisms by which glycine affects mitochondrial function during oocyte maturation in vitro have not been fully investigated. In this study, we induced a mitochondrial damage model in oocytes with the Bcl-2-specific antagonist ABT-199. We investigated whether glycine could reverse the mitochondrial dysfunction induced by ABT-199 exposure and whether it is related to calcium regulation. Our results showed that ABT-199 inhibited cumulus expansion, decreased the oocyte maturation rate and the intracellular glutathione (GSH) level, caused mitochondrial dysfunction, induced oxidative stress, which was confirmed by decreased mitochondrial membrane potential (Δ⍦m) and the expression of mitochondrial function-related genes (PGC-1α), and increased reactive oxygen species (ROS) levels and the expression of apoptosis-associated genes (Bax, caspase-3, CytC). More importantly, ABT-199-treated oocytes showed an increase in the intracellular free calcium concentration ([Ca 2+]i) and had impaired cortical type 1 inositol 1,4,5-trisphosphate receptors (IP3R1) distribution. Nevertheless, treatment with glycine significantly ameliorated mitochondrial dysfunction, oxidative stress and apoptosis, glycine also regulated [Ca 2+]i levels and IP3R1 cellular distribution, which further protects oocyte maturation in ABT-199-induced porcine oocytes. Taken together, our results indicate that glycine has a protective action against ABT-199-induced mitochondrial dysfunction in porcine oocytes.

scholarly journals Chronological Changes of Bovine Follicular Oocyte Maturation in Vitro

1986 ◽  
Vol 27 (4) ◽  
pp. 505-519 ◽  
Author(s):  
K. P. Xu ◽  
T. Greve ◽  
S. Smith ◽  
P. Hyttel
Keyword(s):  
Oocyte Maturation ◽  
Maturation In Vitro

Weightlessness affects cytoskeleton of rat utricular hair cells during maturation in vitro

Neuroreport ◽  
2002 ◽  
Vol 13 (16) ◽  
pp. 2139-2142 ◽  
Author(s):  
Sophie Gaboyard ◽  
Marie-Pierre Blanchard ◽  
C??cile Travo ◽  
Michel Viso ◽  
Alain Sans ◽  
...  
Keyword(s):  
Hair Cells ◽  
Maturation In Vitro
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