Evidence for Linkage between Human Loci for 6-Phosphogluconate Dehydrogenase and Phosphoglucomutase1 in Man–Chinese Hamster Somatic Cell Hybrids

Nature ◽  
1972 ◽  
Vol 236 (5340) ◽  
pp. 30-32 ◽  
Author(s):  
A. WESTERVELD ◽  
P. MEERA KHAN
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Phosphogluconate Dehydrogenase ◽  
Cell Hybrids

Segregation of recessive phenotypes in somatic cell hybrids: role of mitotic recombination, gene inactivation, and chromosome nondisjunction

1981 ◽  
Vol 1 (4) ◽  
pp. 336-346
Author(s):  
C E Campbell ◽  
R G Worton
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Mitotic Recombination ◽  
Chinese Hamster Cell ◽  
Gene Inactivation ◽  
Chromosome Loss ◽  
Resistance Locus ◽  
Chromosome 2 ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Somatic cell hybrids heterozygous at the emetine resistance locus (emtr/emt+) or the chromate resistance locus (chrr/chr+) are known to segregate the recessive drug resistance phenotype at high frequency. We have examined mechanisms of segregation in Chinese hamster cell hybrids heterozygous at these two loci, both of which map to the long arm of Chinese hamster chromosome 2. To follow the fate of chromosomal arms through the segregation process, our hybrids were also heterozygous at the mtx (methotrexate resistance) locus on the short arm of chromosome 2 and carried cytogenetically marked chromosomes with either a short-arm deletion (2p-) or a long-arm addition (2q+). Karyotype and phenotype analysis of emetine- or chromate-resistant segregants from such hybrids allowed us to distinguish four potential segregation mechanisms: (i) loss of the emt+- or chr+-bearing chromosome; (ii) mitotic recombination between the centromere and the emt or chr loci, giving rise to homozygous resistant segregants; (iii) inactivation of the emt+ or chr+ alleles; and (iv) loss of the emt+- or chr+-bearing chromosome with duplication of the homologous chromosome carrying the emtr or chrr allele. Of 48 independent segregants examined, only 9 (20%) arose by simple chromosome loss. Two segregants (4%) were consistent with a gene inactivation mechanism, but because of their rarity, other mechanisms such as mutation or submicroscopic deletion could not be excluded. Twenty-one segregants (44%) arose by either mitotic recombination or chromosome loss and duplication; the two mechanisms were not distinguishable in that experiment. Finally, in hybrids allowing these two mechanisms to be distinguished, 15 segregants (31%) arose by chromosome loss and duplication, and none arose by mitotic recombination.

Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids

1973 ◽  
Vol 20 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Ans Jongsma ◽  
Harry Someren ◽  
Andries Westerveld ◽  
Ann Hagemeijer ◽  
Peter Pearson
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Retention of both parental mitochondrial DNA species in mouse-Chinese hamster somatic cell hybrids

1984 ◽  
Vol 10 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Steven H. Zuckerman ◽  
Joseph F. Solus ◽  
Frances P. Gillespie ◽  
Jerome M. Eisenstadt
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Cell ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Genetical Studies on the Multiple Forms of Human Guanylate Kinase in Man-Chinese Hamster Somatic Cell Hybrids

1974 ◽  
Vol 24 (5-6) ◽  
pp. 415-423 ◽  
Author(s):  
Meera Khan ◽  
W.R.T. Los ◽  
P.L. Pearson ◽  
A. Westerveld ◽  
D. Bootsma
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Multiple Forms ◽  
Guanylate Kinase ◽  
Cell Hybrids
Sign in / Sign up

Export Citation Format

Share Document