X-ray-induced Morphological Differentiation of Mouse Neuroblastoma Cells in vitro

Nature ◽  
1971 ◽  
Vol 234 (5330) ◽  
pp. 471-473 ◽  
Author(s):  
KEDAR N. PRASAD
Keyword(s):  
Neuroblastoma Cells ◽  
Morphological Differentiation ◽  
X Ray ◽  
Mouse Neuroblastoma

scholarly journals Proteins from morphologically differentiated neuroblastoma cells promote tubulin polymerization.

1978 ◽  
Vol 76 (2) ◽  
pp. 547-555 ◽  
Author(s):  
N W Seeds ◽  
R B Maccioni
Keyword(s):  
High Speed ◽  
Gradient Centrifugation ◽  
Neuroblastoma Cells ◽  
Morphological Differentiation ◽  
Tubulin Polymerization ◽  
Associated Proteins ◽  
Neurite Formation ◽  
Brain Microtubules ◽  
Brain Tubulin

Clonal cells (N18) of the mouse neuroblastoma C-1300 can be induced to undergo a morphological differentiation characterized by the outgrowth of very long neurites (> 150 microns) that contain many microtubules. Because the marked increase in the number and length of microtubules is apparently not due to an increase in the concentration of tubulin subunits, the possible role of additional macromolecules in the regulation of tubulin polymerization during neurite formation by N18 cells was examined. Using an in vitro system where the polymerization of low concentrations (< 4 mg/ml) of purified brain tubulin requires microtubule-associated proteins (MAPs), high-speed supernates (250,000 g) from neuroblastoma and glioma cells were assayed for their ability to replace MAPs in the polymerization of brain tubulin. Only the supernates from "differentiated" N18 cells were polymerization competent. Electron microscope observations of these supernates failed to demonstrate the presence of nucleation structures (rings or disks). The active factor(s) sedimented at approximately 7S on sucrose gradient centrifugation and eluted from 4B Sepharose in the region of 170,000 mol wt proteins. Furthermore, the inactive supernates from other cells did not inhibit polymerization when tested in the presence of limiting MAPs. Thus, microtubule formation accompanying neurite outgrowth in neuroblastoma cells appears to be regulated by the presence of additional macromolecular factor(s) that may be functionally equivalent to the MAPs found with brain microtubules.

Comparative effects of the metabolic inhibitors 2,4-dinitrophenol and iodoacetate on mouse neuroblastoma cells in vitro

Toxicology ◽  
1996 ◽  
Vol 110 (1-3) ◽  
pp. 123-132 ◽  
Author(s):  
Maria Isabel Andres ◽  
Guillermo Repetto ◽  
Pilar Sanz ◽  
Manuel Repetto
Keyword(s):  
Neuroblastoma Cells ◽  
Metabolic Inhibitors ◽  
Mouse Neuroblastoma

In vitro effects of thallium on mouse neuroblastoma cells

1994 ◽  
Vol 8 (4) ◽  
pp. 609-611 ◽  
Author(s):  
G. Repetto ◽  
P. Sanz ◽  
M. Repetto
Keyword(s):  
Neuroblastoma Cells ◽  
Mouse Neuroblastoma ◽  
In Vitro Effects

Comparative effects of three chromium compounds on mouse neuroblastoma cells cultured in vitro

1996 ◽  
Vol 88 ◽  
pp. 41-42 ◽  
Author(s):  
Guillermo Repetto ◽  
Ana del Peso ◽  
Manuel Salguero ◽  
Antonio Garfía ◽  
Pilar Sanz ◽  
...  
Keyword(s):  
Neuroblastoma Cells ◽  
Mouse Neuroblastoma ◽  
Chromium Compounds ◽  
Cells Cultured

Biochemical effects of 2,4-dinitrophenol and iodoacetate on mouse neuroblastoma cells in vitro

1996 ◽  
Vol 88 ◽  
pp. 40
Author(s):  
María Isabel Andrés ◽  
Guillermo Repetto ◽  
Pilar Sanz ◽  
Manuel Repetto
Keyword(s):  
Neuroblastoma Cells ◽  
Biochemical Effects ◽  
Mouse Neuroblastoma

scholarly journals Up-regulation of the integrin alpha 1/beta 1 in human neuroblastoma cells differentiated by retinoic acid: correlation with increased neurite outgrowth response to laminin.

1991 ◽  
Vol 2 (12) ◽  
pp. 1021-1033 ◽  
Author(s):  
P Rossino ◽  
P Defilippi ◽  
L Silengo ◽  
G Tarone
Keyword(s):  
Retinoic Acid ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cells ◽  
Morphological Differentiation ◽  
Laminin Receptor ◽  
Human Neuroblastoma Cell ◽  
Type I ◽  
Human Neuroblastoma ◽  
Beta 1 Integrin

Retinoic acid (RA) is known to induce differentiation of neuroblastoma cells in vitro. Here we show that treatment of two human neuroblastoma cell lines, SY5Y and IMR32, with RA resulted in a fivefold increase of the integrin alpha 1/beta 1 expression. The effect was selective because expression of the alpha 3/beta 1 integrin, also present in these cells, was not increased. The up-regulation of the alpha 1/beta 1 differentiated SY5Y cells correlated with increased neurite response to laminin. In fact, RA-treated SY5Y cells elongated neurites on laminin-coated substratum more efficiently compared with untreated cells or cells treated with nerve growth factor, insulin, or phorbol 12-myristate 13-acetate. These three agents induced partial morphological differentiation but did not increase alpha 1 integrin expression. Neurite extension in RA-treated cells was more efficient on laminin than on fibronectin or collagen type I and was inhibited with beta 1 integrin antibodies on all three substrates. Affinity chromatography experiments showed that alpha 1/beta 1 is the major laminin receptor in both untreated and RA-treated SY5Y cells. These data show that RA, a naturally occurring morphogen implicated in embryonic development, can selectively regulate the expression of integrin complexes in neuronal cells and suggest an important role of the alpha 1/beta 1 laminin receptor in the morphological differentiation of nerve cells.

Morphological differentiation of NIE-115 mouse neuroblastoma cells

1997 ◽  
Vol 27 (5) ◽  
pp. 516-523 ◽  
Author(s):  
M. A. Kostenko ◽  
S. N. Myakisheva ◽  
V. I. Popov
Keyword(s):  
Neuroblastoma Cells ◽  
Morphological Differentiation ◽  
Mouse Neuroblastoma
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