Amino-acids, Aliphatic and Aromatic Hydrocarbons in the Murchison Meteorite

Nature ◽  
1971 ◽  
Vol 230 (5289) ◽  
pp. 105-106 ◽  
Author(s):  
J. ORÓ ◽  
J. GIBERT ◽  
H. LICHTENSTEIN ◽  
S. WIKSTROM ◽  
D. A. FLORY
Keyword(s):  
Amino Acids ◽  
Aromatic Hydrocarbons ◽  
Murchison Meteorite ◽  
Aliphatic And Aromatic Hydrocarbons

scholarly journals Directed Evolution of Biphenyl Dioxygenase: Emergence of Enhanced Degradation Capacity for Benzene, Toluene, and Alkylbenzenes

2001 ◽  
Vol 183 (18) ◽  
pp. 5441-5444 ◽  
Author(s):  
Hikaru Suenaga ◽  
Mariko Mitsuoka ◽  
Yuko Ura ◽  
Takahito Watanabe ◽  
Kensuke Furukawa
Keyword(s):  
Amino Acids ◽  
Aromatic Hydrocarbons ◽  
Burkholderia Cepacia ◽  
Large Subunit ◽  
Site Directed Mutagenesis ◽  
Biphenyl Dioxygenase ◽  
Pseudomonas Pseudoalcaligenes ◽  
Benzene Toluene ◽  
Related Compounds ◽  
Enhanced Degradation

ABSTRACT Biphenyl dioxygenase (Bph Dox) catalyzes the initial oxygenation of biphenyl and related compounds. Bph Dox is a multicomponent enzyme in which a large subunit (encoded by the bphA1 gene) is significantly responsible for substrate specificity. By using the process of DNA shuffling of bphA1 of Pseudomonas pseudoalcaligenes KF707 and Burkholderia cepaciaLB400, a number of evolved Bph Dox enzymes were created. Among them, anEscherichia coli clone expressing chimeric Bph Dox exhibited extremely enhanced benzene-, toluene-, and alkylbenzene-degrading abilities. In this evolved BphA1, four amino acids (H255Q, V258I, G268A, and F277Y) were changed from the KF707 enzyme to those of the LB400 enzyme. Subsequent site-directed mutagenesis allowed us to determine the amino acids responsible for the degradation of monocyclic aromatic hydrocarbons.

Polycyclic aromatic hydrocarbons in the Murchison meteorite

1984 ◽  
Vol 5 (4) ◽  
pp. 211-216 ◽  
Author(s):  
Brenda P. Basile ◽  
Brian S. Middleditch ◽  
J. Oró
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Murchison Meteorite ◽  
Polycyclic Aromatic

Characterization of hydrocarbon fluid inclusions by infra-red and fluorescence microspectrometry

1990 ◽  
Vol 54 (375) ◽  
pp. 311-324 ◽  
Author(s):  
Nicole Guilhaumou ◽  
Nathalie Szydlowskii ◽  
Bernard Pradier
Keyword(s):  
Fluid Inclusions ◽  
Aromatic Hydrocarbons ◽  
Fluorescence Emission ◽  
Liquid Hydrocarbon ◽  
Fluorescence Emission Spectrum ◽  
Hydrocarbon Fluid ◽  
Liquid Phases ◽  
Infra Red ◽  
Aliphatic And Aromatic Hydrocarbons

AbstractLiquid-hydrocarbon-bearing fluid inclusions have often been described associated with petroleum occurrences and diagenetic sediments. Infra-red microspectrometry allows characterization of fluid inclusions greater than 20 µm by establishing the presence of aliphatic and aromatic hydrocarbons as well as associated H2O, CO2 and CH4. Semi-quantitative analyses have been made by focussing on gaseous and liquid phases separately. Some CH2/CH3 and CO2/CH4 ratios have been determined by this method.Fluorescence microspectrometry permits precise measurements of the fluorescence emission spectrum of chromophore-bearing organic phases (essentially aromatic hydrocarbons) in fluid inclusions greater than 10 µm. Such a spectrum is a function of both the gross composition of the trapped oil and its thermal history.Both of these methods lead to the in situ characterization of hydrocarbon fluid inclusions. They are useful in providing a quantifiable distinction between different oil generations trapped during mineral growth in diagenetic and epigenetic minerals.

Sign in / Sign up

Export Citation Format

Share Document