Established Insect Cell Line from the Cabbage Looper, Trichoplusia ni

Nature ◽  
1970 ◽  
Vol 226 (5244) ◽  
pp. 466-467 ◽  
Author(s):  
W. F. HINK
Keyword(s):  
Cell Line ◽  
Insect Cell ◽  
Trichoplusia Ni ◽  
Insect Cell Line ◽  
Cabbage Looper

scholarly journals Genome Assembly and Annotation of the Trichoplusia ni Tni-FNL Insect Cell Line Enabled by Long-Read Technologies

Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 79 ◽  
Author(s):  
Keyur Talsania ◽  
Monika Mehta ◽  
Castle Raley ◽  
Yuliya Kriga ◽  
Sujatha Gowda ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Cell Line ◽  
Genome Assembly ◽  
Insect Cell ◽  
Trichoplusia Ni ◽  
Genome Engineering ◽  
Insect Cell Line ◽  
Baculovirus Infection ◽  
Long Read

Background: Trichoplusia ni derived cell lines are commonly used to enable recombinant protein expression via baculovirus infection to generate materials approved for clinical use and in clinical trials. In order to develop systems biology and genome engineering tools to improve protein expression in this host, we performed de novo genome assembly of the Trichoplusia ni-derived cell line Tni-FNL. Methods: By integration of PacBio single-molecule sequencing, Bionano optical mapping, and 10X Genomics linked-reads data, we have produced a draft genome assembly of Tni-FNL. Results: Our assembly contains 280 scaffolds, with a N50 scaffold size of 2.3 Mb and a total length of 359 Mb. Annotation of the Tni-FNL genome resulted in 14,101 predicted genes and 93.2% of the predicted proteome contained recognizable protein domains. Ortholog searches within the superorder Holometabola provided further evidence of high accuracy and completeness of the Tni-FNL genome assembly. Conclusions: This first draft Tni-FNL genome assembly was enabled by complementary long-read technologies and represents a high-quality, well-annotated genome that provides novel insight into the complexity of this insect cell line and can serve as a reference for future large-scale genome engineering work in this and other similar recombinant protein production hosts.

Reaction of an insect cell line to ecdysterone

1971 ◽  
Vol 64 (2) ◽  
pp. 335-338 ◽  
Author(s):  
J.P. Reinecke ◽  
J.D. Robbins
Keyword(s):  
Cell Line ◽  
Insect Cell ◽  
Insect Cell Line

Two very different components of messenger RNA in an insect cell line

Cell ◽  
1975 ◽  
Vol 4 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Allan Spradling ◽  
Helen Hui ◽  
Sheldon Penman
Keyword(s):  
Cell Line ◽  
Insect Cell ◽  
Messenger Rna ◽  
Insect Cell Line

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium

Adaptation of an Insect Cell Line (Grace's Antheraea Cells) to Medium Free of Insect Hemolymph

Science ◽  
1967 ◽  
Vol 155 (3769) ◽  
pp. 1565-1566 ◽  
Author(s):  
C. E. Yunker ◽  
J. L. Vaughn ◽  
J. Cory
Keyword(s):  
Cell Line ◽  
Insect Cell ◽  
Insect Cell Line
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