Demonstration of an Internal Structure within the Red Blood Cell by Ion Etching and Scanning Electron Microscopy

Nature ◽  
1968 ◽  
Vol 220 (5167) ◽  
pp. 614-616 ◽  
Author(s):  
S. M. LEWIS ◽  
J. S. OSBORN ◽  
P. R. STUART
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Internal Structure ◽  
Red Blood Cell ◽  
Ion Etching ◽  
Scanning Electron

scholarly journals The effects of zinc on the morphology of sickle red blood cell ghosts as observed by scanning electron microscopy

1977 ◽  
Vol 170 (2) ◽  
pp. 149-159 ◽  
Author(s):  
W. C. Kruckeberg ◽  
F. J. Oelshlegel ◽  
S. H. Shore ◽  
P. E. Smouse ◽  
G. J. Brewer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Scanning Electron

Scanning Electron Microscopy Reveals Two Distinct Classes of Erythroblastic Island Isolated from Adult Mammalian Bone Marrow

2016 ◽  
Vol 22 (2) ◽  
pp. 368-378 ◽  
Author(s):  
Jia Hao Yeo ◽  
Bronwyn M. McAllan ◽  
Stuart T. Fraser
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Surface Proteins ◽  
Erythroid Cell ◽  
Erythroid Progenitors ◽  
Hematological Disorders ◽  
And Function ◽  
Scanning Electron

AbstractErythroblastic islands are multicellular clusters in which a central macrophage supports the development and maturation of red blood cell (erythroid) progenitors. These clusters play crucial roles in the pathogenesis observed in animal models of hematological disorders. The precise structure and function of erythroblastic islands is poorly understood. Here, we have combined scanning electron microscopy and immuno-gold labeling of surface proteins to develop a better understanding of the ultrastructure of these multicellular clusters. The erythroid-specific surface antigen Ter-119 and the transferrin receptor CD71 exhibited distinct patterns of protein sorting during erythroid cell maturation as detected by immuno-gold labeling. During electron microscopy analysis we observed two distinct classes of erythroblastic islands. The islands varied in size and morphology, and the number and type of erythroid cells interacting with the central macrophage. Assessment of femoral marrow isolated from a cavid rodent species (guinea pig,Cavis porcellus) and a marsupial carnivore species (fat-tailed dunnarts,Sminthopsis crassicaudata) showed that while the morphology of the central macrophage varied, two different types of erythroblastic islands were consistently identifiable. Our findings suggest that these two classes of erythroblastic islands are conserved in mammalian evolution and may play distinct roles in red blood cell production.

scholarly journals Combining Scanning Electron Microscopy and Light Microscopy to Study How Blood Cell Directed-architecture Influences Nanoparticle Penetration into Clots

2020 ◽  
Vol 26 (S2) ◽  
pp. 2258-2261
Author(s):  
Cammy Truong ◽  
Courtney Cazzola ◽  
Sara Benzow ◽  
Austen Norberg ◽  
Ashlee Chramega ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Light Microscopy ◽  
Scanning Electron

The use of plasma etching to reveal the internal structure of Nippostrongylus brasiliensis (Nematoda)

Parasitology ◽  
1983 ◽  
Vol 86 (3) ◽  
pp. 477-480 ◽  
Author(s):  
D. L. Lee ◽  
C. D. Nicholls
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Internal Structure ◽  
Plasma Etching ◽  
Middle Layer ◽  
Nippostrongylus Brasiliensis ◽  
Cortical Layers ◽  
Transmission Electron ◽  
Scanning Electron

SUMMARYPlasma etching has been used to strip away the cortical layers of the cuticle of adult Nippostrongylus brasiliensis to reveal the struts, with their supporting fibres, which are found in the fluid-filled middle layer of the cuticle, and the basal fibre layers. The etched specimens were studied by means of scanning electron microscopy. The results support earlier work, obtained by transmission electron microscopy, on the cuticle of this nematode. Plasma etching has been shown to have potential in studying the structure of nematodes.

Microscopy and Microanalysis of Blood in a Snake Head Fish, Channa gachua Exposed to Environmental Pollution

2016 ◽  
Vol 22 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Eva M. Pala ◽  
Sudip Dey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Analytical Methods ◽  
Analytical Approach ◽  
Erythrocyte Membranes ◽  
X Ray ◽  
Structural Abnormalities ◽  
Polluted Sites ◽  
Scanning Electron

AbstractConventional and highly sophisticated analytical methods (Cyria et al., 1989; Massar et al., 2012a) were used to analyze micro-structural and micro-analytical aspects of the blood of snake head fish, Channa gachua, exposed to municipal wastes and city garbage. Red (RBC) and white blood cell (WBC) counts and hemhemoglobin content were found to be higher in pollution affected fish as compared with control. Scanning electron microscopy revealed the occurrence of abnormal erythrocytes such as crenated cells, echinocytes, lobopodial projections, membrane internalization, spherocytes, ruptured cells, contracted cells, depression, and uneven elongation of erythrocyte membranes in fish inhabiting the polluted sites. Energy-dispersive X-ray spectroscopy (EDS) revealed the presence of silicon and lead in the RBCs of pollution affected fish. Significance of the study includes the highly sophisticated analytical approach, which revealed the aforementioned micro-structural abnormalities.

Sign in / Sign up

Export Citation Format

Share Document