Ethylene produced by Plant Cells in Suspension Cultures

Nature ◽  
1968 ◽  
Vol 220 (5167) ◽  
pp. 604-605 ◽  
Author(s):  
O. L. GAMBORG ◽  
T. A. G. LARUE
Keyword(s):  
Plant Cells ◽  
Suspension Cultures

scholarly journals Biotransformation of Cyclodextrine-Complexed Semisynthetic Betulin Derivatives by Plant Cells

Planta Medica ◽  
2018 ◽  
Vol 84 (09/10) ◽  
pp. 743-748
Author(s):  
Suvi Häkkinen ◽  
Heli Nygren ◽  
Natalia Maiorova ◽  
Raisa Haavikko ◽  
Sami Alakurtti ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Plant Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Water Soluble ◽  
Betulonic Acid ◽  
Poorly Water Soluble ◽  
Betulin Derivatives

AbstractIn this study, three semisynthetic betulonic acid-based compounds, 20(29)-dihydrolup-2-en[2,3-d]isoxazol-28-oic acid, 1-betulonoylpyrrolidine, and lupa-2,20(29)-dieno[2,3-b]pyrazin-28-oic acid, were studied in biotransformation experiments using Nicotiana tabacum and Catharanthus roseus cell suspension cultures. Biotransformation was performed using cyclodextrin to aid dissolving poorly water-soluble substrates. Several new derivatives were found, consisting of oxidized and glycosylated (pentose- and hexose-conjugated) products.

Growth of suspension cultures of plant cells (Ipomoea sp.) at various temperatures

1975 ◽  
Vol 53 (4) ◽  
pp. 315-320 ◽  
Author(s):  
Dyson Rose ◽  
S. M. Martin
Keyword(s):  
Growth Rate ◽  
Suspension Culture ◽  
Cell Line ◽  
Plant Cells ◽  
Maximum Growth ◽  
Suspension Cultures ◽  
Root Explants ◽  
Temperature Variations ◽  
Satisfactory Method ◽  
Dry Cell

Studies on the growth of batch suspension cultures of a plant cell line, initiated from Ipomoea sp. root explants, at temperatures ranging from 15 to 34 °C are reported. Maximum growth of cultures of this cell line occurred between 25 and 32 °C, with temperature variations within this range having little effect on growth rates based on dry cell yields. Cultivation at 20 °C substantially reduced the growth rate. However, cells grown at 20 °C failed to grow on transfer to fresh medium at 20 °C. Thus cultivation at suboptimum temperatures is not a satisfactory method for routine maintenance of the suspension culture.

Preservation of suspension cultures of plant cells by freezing

1971 ◽  
Vol 49 (7) ◽  
pp. 1253-1254 ◽  
Author(s):  
R. Latta
Keyword(s):  
Daucus Carota ◽  
Plant Cells ◽  
Suspension Cultures ◽  
Protective Agent ◽  
Short Period ◽  
Conventional Medium

Development of a method of preserving suspension cultures of plant cells has been undertaken. When grown in a conventional medium containing 20 g/1 of sucrose, Daucus carota cells survived freezing and a short period of storage at −40 °C or −196 °C in the presence of a suitable protective agent, whereas Ipomoea sp. cells did not. Ipomoea cells survived freezing if they were adapted to growth in a medium containing 65 g/1 of sucrose.

VARIATIONS IN CHROMOSOME NUMBER AND STRUCTURE IN PLANT CELLS GROWN IN SUSPENSION CULTURES

1970 ◽  
Vol 12 (2) ◽  
pp. 297-301 ◽  
Author(s):  
K. N. Kao ◽  
R. A. Miller ◽  
O. L. Gamborg ◽  
B. L. Harvey
Keyword(s):  
Glycine Max ◽  
Chromosome Number ◽  
Cell Suspension ◽  
Plant Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Triticum Monococcum ◽  
Melilotus Alba ◽  
Haplopappus Gracilis ◽  
Chromosomal Changes

Cell suspension cultures of Triticum monococcum, Triticiam aestivum, Glycine max, Melilotus alba, and Haplopappus gracilis, were examined to determine whether chromosomal changes had occurred during culture. All cultures except H. gracilis showed change in chromosome number and the two species of Triticum showed abnormal karyotypes.

The effect of physical stress on plant cells in suspension cultures

1988 ◽  
Vol 10 (7) ◽  
pp. 485-490 ◽  
Author(s):  
H. Tanaka ◽  
H. Semba ◽  
T. Jitsufuchi ◽  
H. Harada
Keyword(s):  
Plant Cells ◽  
Suspension Cultures ◽  
Physical Stress

Studies on the Growth in Culture of Plant Cells: I. GROWTH PATTERNS IN BATCH PROPAGATED SUSPENSION CULTURES

1966 ◽  
Vol 17 (2) ◽  
pp. 362-377 ◽  
Author(s):  
G. G. HENSHAW ◽  
K. K. JHA ◽  
A. R. MEHTA ◽  
D. JOAN SHADESHAFT ◽  
H.E. STREET
Keyword(s):  
Plant Cells ◽  
Suspension Cultures ◽  
Growth Patterns

scholarly journals Regioselective hydroxylation and dehydrogenation of capsaicin and dihydrocapsaicin by cultured cells of Phytolacca americana

2021 ◽  
Vol 85 (1) ◽  
pp. 103-107
Author(s):  
Kei Shimoda ◽  
Tsubasa Ono ◽  
Hiroki Hamada
Keyword(s):  
Cultured Cells ◽  
Plant Cells ◽  
Suspension Cultures ◽  
The Other ◽  
Phytolacca Americana ◽  
Other Hand ◽  
Regioselective Hydroxylation ◽  
First Time

Abstract The biotransformations of capsaicin and dihydrocapsaicin were investigated using cultured plant cells of Phytolacca americana as biocatalysts. Four products, ie 15-hydroxycapsaicin, dihydrocapsaicin, 15-hydroxydihydrocapsaicin, and capsaicin 4-β-glucoside, were isolated from the suspension cultures of P. americana treated with capsaicin for 3 days, showing that capsaicin was regioselectively hydroxylated, reduced, and glucosylated by cultured P. americana cells. On the other hand, dihydrocapsaicin was regioselectively dehydrogenated, hydroxylated, reduced, and glucosylated to give four products, ie capsaicin, 15-hydroxycapsaicin, 15-hydroxydihydrocapsaicin, and capsaicin 4-β-glucoside, by cultured P. americana cells. In this paper, it is reported, for the first time, that dihydrocapsaicin is converted into 15-hydroxydihydrocapsaicin by plant cultured cells.

Surface-associated plant cell culture

2020 ◽  
Author(s):  
Alexander Mehring ◽  
Judith Stiefelmaier ◽  
Roland Ulber
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Plant Cell ◽  
Plant Cell Culture ◽  
Extracellular Polymeric Substances ◽  
Plant Cells ◽  
Suspension Cultures ◽  
Extracellular Polysaccharides ◽  
Adhesive Properties ◽  
German Research Foundation

<p>Biofilms are typically characterized as a consortium of microorganisms, which adhere to each other and often to surfaces. This adhesion is realized by extracellular polymeric substances (EPS), which are secreted by the microorganisms and mainly consist of water, polysaccharides, proteins and lipids as well as nucleic acids and lysis products [1]. Although cultured plant cells are not typically considered biofilms, parallels can be found in the properties of plant calli. These callus cells tend to form cohesive aggregates, owing to their extracellular matrix, and often strongly adhere to the agar plates they are kept on. The extracellular matrix of plant cells is mainly composed of structural polysaccharides, such as xyloglucans, arabinogalactans [2], homogalacturonan and extensins [3] among others. Cultured plant cells were found to adhere to surfaces before [4]. Surface-associated plant cell culture may have potential in a (semi‑)continuous cultivation including product secretion, as was shown in principle for alginate-embedded plant cells [5]. For cyanobacterial biofilms, an efficient strategy for EPS extraction was recently developed [6]. The transferability of these protocols to biofilm-like growing plant calli of Ocimum basilicum is currently being investigated. Subsequently, the composition of the extracellular matrix extracted from cultured O. basilicum cells is of interest. Furthermore, the adhesive properties of O. basilicum suspension cultures to microstructured surfaces and the potential role of the extracellular matrix are under investigation. An investigation of culture properties in an aerosol photobioreactor [7] is planned as well.</p> <p>This project is financially supported by the German research foundation (DFG, project number SFB 926-C03).</p> <p> </p> <p>References:</p> <p>[1]      H. C. Flemming, T. R. Neu, and D. J. Wozniak, “The EPS matrix: The ‘House of Biofilm Cells,’” J. Bacteriol., vol. 189, no. 22, pp. 7945–7947, 2007.</p> <p>[2]      I. M. Sims, K. Middleton, A. G. Lane, A. J. Cairns, and A. Bacic, “Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae,” Planta, vol. 210, no. 2, pp. 261–268, Jan. 2000.</p> <p>[3]      M. Popielarska-Konieczna, K. Sala, M. Abdullah, M. Tuleja, and E. Kurczyńska, “Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta,” Plant Cell Rep., no. 0123456789, 2020.</p> <p>[4]      R. J. Robins, D. O. Hall, D. ‐J Shi, R. J. Turner, and M. J. C. Rhodes, “Mucilage acts to adhere cyanobacteria and cultured plant cells to biological and inert surfaces,” FEMS Microbiol. Lett., vol. 34, no. 2, pp. 155–160, 1986.</p> <p>[5]      Y. Kobayashi, H. Fukui, and M. Tabata, “Berberine production by batch and semi-continuous cultures of immobilized Thalictrum cells in an improved bioreactor,” Plant Cell Rep., vol. 7, no. 4, pp. 249–252, 1988.</p> <p>[6]      D. Strieth, J. Stiefelmaier, B. Wrabl et al., “A new strategy for a combined isolation of EPS and pigments from cyanobacteria,” J. Appl. Phycol., no. Fromme 2008, Feb. 2020.</p> <p>[7]        S. Kuhne, D. Strieth, M. Lakatos, K. Muffler, and R. Ulber, “A new photobioreactor concept enabling the production of desiccation induced biotechnological products using terrestrial cyanobacteria,” J. Biotechnol., vol. 192, no. Part A, pp. 28–33, 2014.</p>

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