Peptidyl Transferase

Nature ◽  
1968 ◽  
Vol 219 (5154) ◽  
pp. 560-560
Author(s):  
Keyword(s):  
Peptidyl Transferase

scholarly journals Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Hauke S. Hillen ◽  
Elena Lavdovskaia ◽  
Franziska Nadler ◽  
Elisa Hanitsch ◽  
Andreas Linden ◽  
...  
Keyword(s):  
Ribosomal Proteins ◽  
Ribosome Biogenesis ◽  
Ribosomal Subunit ◽  
Structural Basis ◽  
Peptidyl Transferase ◽  
Mitochondrial Ribosomes ◽  
Mitochondrial Ribosome ◽  
In Vitro Reconstitution

AbstractRibosome biogenesis requires auxiliary factors to promote folding and assembly of ribosomal proteins and RNA. Particularly, maturation of the peptidyl transferase center (PTC) is mediated by conserved GTPases, but the molecular basis is poorly understood. Here, we define the mechanism of GTPase-driven maturation of the human mitochondrial large ribosomal subunit (mtLSU) using endogenous complex purification, in vitro reconstitution and cryo-EM. Structures of transient native mtLSU assembly intermediates that accumulate in GTPBP6-deficient cells reveal how the biogenesis factors GTPBP5, MTERF4 and NSUN4 facilitate PTC folding. Addition of recombinant GTPBP6 reconstitutes late mtLSU biogenesis in vitro and shows that GTPBP6 triggers a molecular switch and progression to a near-mature PTC state. Additionally, cryo-EM analysis of GTPBP6-treated mature mitochondrial ribosomes reveals the structural basis for the dual-role of GTPBP6 in ribosome biogenesis and recycling. Together, these results provide a framework for understanding step-wise PTC folding as a critical conserved quality control checkpoint.

Structure of a conserved RNA component of the peptidyl transferase centre

1997 ◽  
Vol 4 (10) ◽  
pp. 775-778 ◽  
Author(s):  
Elisabetta Viani Puglisi ◽  
Rachel Green ◽  
Harry F. Noller ◽  
Joseph D. Puglisi
Keyword(s):  
Peptidyl Transferase ◽  
Peptidyl Transferase Centre

Peptidyl Transferase Center of Rat-Liver Ribosome Cores

1977 ◽  
Vol 73 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Ramon REYES ◽  
David VAZQUEZ ◽  
Juan P. G. BALLESTA
Keyword(s):  
Rat Liver ◽  
Peptidyl Transferase ◽  
Peptidyl Transferase Center

scholarly journals Peptidyl Transferase Center and the Emergence of the Translation System

Life ◽  
2017 ◽  
Vol 7 (2) ◽  
pp. 21 ◽  
Author(s):  
Savio Torres de Farias ◽  
Thais Gaudêncio Rêgo ◽  
Marco José
Keyword(s):  
Translation System ◽  
Peptidyl Transferase ◽  
Peptidyl Transferase Center
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