Properties and Composition of the Isolated Ribosomal DNA Satellite of Xenopus laevis

Nature ◽  
1968 ◽  
Vol 219 (5153) ◽  
pp. 454-463 ◽  
Author(s):  
M. BIRNSTIEL ◽  
J. SPEIRS ◽  
I. PURDOM ◽  
K. JONES ◽  
U. E. LOENING
Keyword(s):  
Xenopus Laevis ◽  
Ribosomal Dna

The Xenopus laevis ribosomal gene terminator contains sequences that both enhance and repress ribosomal transcription

1989 ◽  
Vol 9 (9) ◽  
pp. 3777-3784
Author(s):  
S Firek ◽  
C Read ◽  
D R Smith ◽  
T Moss
Keyword(s):  
Xenopus Laevis ◽  
Point Mutation ◽  
Ribosomal Dna ◽  
Ribosomal Gene ◽  
Promoter Element ◽  
Base Pairs ◽  
Transcription Terminator ◽  
Upstream Promoter

A DNA segment approximately 200 base pairs upstream of the Xenopus laevis ribosomal promoter acts both as an upstream promoter element that augments transcription and as a transcription terminator. It is, however, unclear to what extent these two activities are related. A segment of the X. laevis ribosomal DNA, containing the terminator and the upstream promoter element, was subjected to point mutation, and the effects of the resulting mutations were investigated by oocyte microinjection. Analysis of 26 point mutants revealed not only sequences that augment 40S transcription but also those that repress it. The sequences that augmented transcription lay within the T3 homology box and also near the site of 3'-end formation. These sequences also played a role in termination. The sequences that repressed transcription lay within the G+C-rich DNA flanking the T3 box. It can be concluded that termination is probably essential but may not be sufficient for the activity of the upstream promoter element.

scholarly journals Replication of Ribosomal DNA in Xenopus laevis

1981 ◽  
Vol 118 (3) ◽  
pp. 585-590 ◽  
Author(s):  
Irene BOZZONI ◽  
Cosima Tatiana BALDARI ◽  
Francesco AMALDI ◽  
Mario BUONGIORNO-NARDELLI
Keyword(s):  
Xenopus Laevis ◽  
Ribosomal Dna

scholarly journals Transcription in cloned spacers of Xenopus laevis ribosomal DNA.

1983 ◽  
Vol 80 (21) ◽  
pp. 6490-6494 ◽  
Author(s):  
G. T. Morgan ◽  
R. H. Reeder ◽  
A. H. Bakken
Keyword(s):  
Xenopus Laevis ◽  
Ribosomal Dna

Restriction analysis of the nontranscribed spacers of Xenopus laevis ribosomal DNA

Cell ◽  
1977 ◽  
Vol 11 (3) ◽  
pp. 599-607 ◽  
Author(s):  
Peter Botchan ◽  
Ronald H. Reeder ◽  
Igor B. Dawid
Keyword(s):  
Xenopus Laevis ◽  
Ribosomal Dna ◽  
Restriction Analysis
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