Detection of Antibodies against Mitochondria by the Fluorescent Antibody Technique with Smears of Rat Liver Cells

Nature ◽  
1967 ◽  
Vol 215 (5109) ◽  
pp. 1480-1481 ◽  
Author(s):  
PH. RÜMKE ◽  
H. A. BUTER
Keyword(s):  
Rat Liver ◽  
Fluorescent Antibody ◽  
Liver Cells ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Rat Liver Cells

scholarly journals THE LOCALIZATION OF AUSTRALIA ANTIGEN BY IMMUNOFLUORESCENCE

1970 ◽  
Vol 131 (2) ◽  
pp. 307-319 ◽  
Author(s):  
Veronica E. Coyne(Zavatone) ◽  
Irving Millman ◽  
James Cerda ◽  
B. J. S. Gerstley ◽  
Thomas London ◽  
...  
Keyword(s):  
Fluorescent Antibody ◽  
Liver Cells ◽  
Infectious Hepatitis ◽  
Buffy Coat ◽  
Fluorescent Antibody Technique ◽  
Australia Antigen ◽  
Antibody Technique ◽  
Biopsy Specimens ◽  
Lymphocyte Cultures ◽  
Fluorescent Particles

We have studied the localization of Australia antigen, a particulate substance associated with hepatitis, by means of the fluorescent antibody technique. Preparations were made from 61 liver biopsy specimens taken from patients with infectious hepatitis, serum hepatitis, and a variety of other diseases. When tested with fluorescein-conjugated rabbit anti-Au(1) antisera all 26 patients who had Au(1) in their serum had specific fluorescence in their liver cells. The fluorescence appeared in three forms: as discrete particles within the nucleus, diffuse fluorescence of the entire nucleus, and fluorescence of the nuclear rim. Occasionally there were also fluorescent particles in the cytoplasm. Other specimens were tested with the fluorescent antibody including a variety of human tissues, buffy coat smears, peripheral lymphocyte cultures, and cells obtained from bile and duodenal drainage. Among these specimens, fluorescence was found in the cytoplasm of a few cells in the bone marrow of two patients with hepatitis and Au(1) in their serum, and in the liver, spleen, mesentery, and testis of one patient with leukemia, chronic hepatitis, and Au(1) in his serum. We have shown that the presence of fluorescent particles in the liver cells is strongly associated with the presence of Au(1) in the serum and the diagnosis of viral hepatitis. We believe that this study adds support to the hypothesis that Australia antigen is an antigenic determinant of a virus capable of causing hepatitis.

Australia Antigen detected in the Nuclei of Liver Cells of Patients with Viral Hepatitis by the Fluorescent Antibody Technique

Nature ◽  
1969 ◽  
Vol 222 (5189) ◽  
pp. 181-184 ◽  
Author(s):  
IRVING MILLMAN ◽  
VERONICA ZAVATONE ◽  
BETTY JANE S. GERSTLEY ◽  
BARUCH S. BLUMBERG
Keyword(s):  
Viral Hepatitis ◽  
Fluorescent Antibody ◽  
Liver Cells ◽  
Fluorescent Antibody Technique ◽  
Australia Antigen ◽  
Antibody Technique

Effects of Hypophysectomy on the Fine Structure of Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 156-157
Author(s):  
Robert R. Cardell
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Rat Liver ◽  
Liver Cell ◽  
Anterior Pituitary ◽  
Liver Cells ◽  
Biochemical Studies ◽  
Liver Functions ◽  
Rat Liver Cells ◽  
And Control

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

Heterogeneity of carboxylesterases in rat liver cells

1992 ◽  
Vol 44 (4) ◽  
pp. 827-829 ◽  
Author(s):  
Rolf Gaustad ◽  
Trond Berg ◽  
Frode Fonnum
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells
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