Organic Phosphorus Content of the Alpha Chains of Chicken Bone Collagen

Nature ◽  
1967 ◽  
Vol 214 (5088) ◽  
pp. 621-622 ◽  
Author(s):  
C. J. FRANCOIS ◽  
M. J. GLIMCHER ◽  
S. M. KRANE
Keyword(s):  
Phosphorus Content ◽  
Organic Phosphorus ◽  
Bone Collagen ◽  
Chicken Bone

scholarly journals Identification of organic phosphorus covalently bound to collagen and non-collagenous proteins of chicken-bone matrix. The presence of O-phosphoserine and O-phosphothreonine in non-collagenous proteins, and their absence from phosphorylated collagen

1979 ◽  
Vol 177 (1) ◽  
pp. 81-98 ◽  
Author(s):  
Lola Cohen-Solal ◽  
Jane B. Lian ◽  
Dora Kossiva ◽  
Melvin J. Glimcher
Keyword(s):  
Amino Acid ◽  
Glutamic Acid ◽  
Proteinase Inhibitors ◽  
Organic Phosphorus ◽  
Bone Matrix ◽  
Bone Collagen ◽  
Insoluble Residue ◽  
Cationic Amino Acid ◽  
Chicken Bone ◽  
Covalently Bound

Non-collagenous phosphoproteins, almost all of which can be extracted in EDTA at neutral pH in the presence of proteinase inhibitors, are identified in the matrix of chicken bone, and are therefore not covalently bound to collagen. Similarly, all the peptides containing γ-carboxyglutamic acid are present in the EDTA extract and none in the insoluble residue, confirming that none is covalently linked to chicken bone collagen. However, organic phosphorus is also found to be present in chicken bone collagen, principally in the α2-chains. Of the total protein-bound organic phosphorus present in chicken bone matrix, approx. 80% is associated with the non-collagenous proteins and 20% with collagen. The soluble non-collagenous proteins contain both O-phosphoserine and O-phosphothreonine and these account for essentially of their organic phosphorus content. In contrast, collagen contains neither O-phosphoserine nor O-phosphothreonine. Indeed, no phosphorylated hydroxy amino acid, phosphoamidated amino acid or phosphorylated sugar could be identified in purified components of collagen, which contain approximately four to five atoms of organic phosphorus per molecule of collagen. Peptides containing organic phosphorus were isolated from partial acid hydrolysates and enzymic digests of purified collagen components, which contain an as-yet-unidentified cationic amino acid. These data, the very high concentrations of glutamic acid in the phosphorylated peptides, and the pH-stability of the organic phosphorus moiety in intact collagen chains strongly suggest that at least part of the organic phosphorus in collagen is present as phosphorylated glutamic acid. This would indicate that the two major chemically different protein fractions in chicken bone matrix that contain organic phosphorus may represent two distinct metabolic pools of organic phosphorus under separate biological control.

MINERALIZATION OF SOIL ORGANIC PHOSPHORUS WITH PARTICULAR REFERENCE TO THE EFFECT OF LIME

1963 ◽  
Vol 43 (1) ◽  
pp. 97-106 ◽  
Author(s):  
R. L. Halstead ◽  
J. M. Lapensee ◽  
K. C. Ivarson
Keyword(s):  
Phosphorus Content ◽  
Organic Phosphorus ◽  
Acid Soils ◽  
Inorganic Phosphorus ◽  
Eastern Canada ◽  
Surface Samples ◽  
Soil Organic Phosphorus ◽  
Repressive Effect ◽  
Partial Sterilization ◽  
Positive Effect

In a laboratory experiment, liming resulted in an average decline of 3.6 per cent in the total organic phosphorus content of incubated surface samples of seven acid soils from eastern Canada. Increases of 2.6 and 5.1 per cent in 1N H2SO4- and 4N HCl-soluble inorganic phosphorus, respectively, and a decrease of 46.4 per cent in NaHCO3-soluble organic phosphorus (pH 8.5) provided further evidence of mineralization of organic phosphorus following liming. There was some evidence, however, that the differences in NaHCO3-soluble organic phosphorus following liming were due only in part to mineralization, since Ca(OH)2 added to a soil just prior to extraction with NaHCO3 had a repressive effect on the solubility of the organic phosphorus compounds.Some mineralization of organic phosphorus occurred when unlimed samples were incubated in the laboratory for 9 months.Marked increases in microbiological activity, as indicated by increased numbers of microorganisms, and increased CO2 and NO3-nitrogen production, were associated with lower values for extractable organic phosphorus following liming. Partial sterilization of samples with toluene lowered biological activity in the unlimed and limed samples. Toluene was found, however, to have a positive effect on release of phosphorus from organic form.

Molecular weights of the α chains of chicken bone collagen by high-speed sedimentation equilibrium

Biochemistry ◽  
1969 ◽  
Vol 8 (6) ◽  
pp. 2609-2615 ◽  
Author(s):  
Elton P. Katz ◽  
Camille J. Francois ◽  
Melvin J. Glimcher
Keyword(s):  
High Speed ◽  
Bone Collagen ◽  
Sedimentation Equilibrium ◽  
Molecular Weights ◽  
Chicken Bone

scholarly journals Effect on a peat soil of application of superphosphate at various rates

1959 ◽  
Vol 31 (1) ◽  
pp. 120-130
Author(s):  
Armi Kaila
Keyword(s):  
Total Phosphorus ◽  
Dry Matter ◽  
Phosphorus Content ◽  
Organic Phosphorus ◽  
Peat Soil ◽  
Available Phosphorus ◽  
Untreated Soil ◽  
Total Phosphorus Content ◽  
Low Phosphorus ◽  
Better Than

The effect on a fen soil of superphosphate applied for 34 years at the annual rates of 0, 100, 200, and 300 kg/ha was studied. The material consisted of samples from a field trial at the Leteensuo Experiment Station. The soil samples from the ploughing layer were collected in autumn 1956, and the hay samples from the crop harvested in 1957. The hay yields from the treatments with 200 or 300 kg/ha of superphosphate were about 7400 kg/ha, and the phosphorus content of the hay 1.69 and 1.85 per cent, respectively. The treatment with 100 kg/ha of superphosphate yielded only about 5100 kg/ha of hay dry matter with a very low phosphorus content, 0.96 per cent. The yield from the untreated plots was almost negligible. Also the biological and chemical soil tests showed that the untreated soil was almost depleted of available phosphorus. The phosphorus conditions in the soil annually treated with 100 kg/ha of superphosphate were not significantly better than in the untreated soil. An annual anplication of 200kg/ha of superphosphate was able to maintain a more satisfactory rate of available phosphorus in the soil, but only the treatment with 300 kg/ha of superphosphate resulted in markedly higher test values than those for the lower treatments. The total phosphorus content of the soil was the higher the larger the amount of superphosphate applied. About 40 to 50 per cent of the differences between the total phosphorus content of the treated and untreated samples was due to organic phosphorus. The potassium content of the hay was the lower the higher the superphosphate treatment. The hay from the treatment with 300 kg/ha of superphosphate contained only 1.25 per cent potassium. The possibility that potassium was a minimum factor in this treatment was discussed.

EVALUATION OF METHODS FOR DETERMINATION OF TOTAL ORGANIC PHOSPHORUS IN CHERNOZEMIC SOILS OF SOUTHERN ALBERTA

1964 ◽  
Vol 44 (3) ◽  
pp. 265-271 ◽  
Author(s):  
J. F. Dormaar
Keyword(s):  
Phosphorus Content ◽  
Organic Phosphorus ◽  
Black Soil ◽  
Parent Material ◽  
Soil Series ◽  
Soil Zone ◽  
Southern Alberta ◽  
Qualitative Work ◽  
Evaluation Of Methods

Two Orthic Chernozemic profiles of each of four parent materials were selected at undisturbed sites within the Brown, Dark Brown, and Thin Black soil zones of southern Alberta. This constituted 24 profiles representing 12 Chernozemic soil series. Material from the Ah and Bm horizons was subjected to nine procedures for estimating total organic phosphorus.The data of this study generally substantiated the usefulness of a modified Kaila–Virtanen method for these soils. With some soils, such as those from the Brown soil zone and those developed from aeolian parent material, other methods gave similar results. Reproducible results were obtained with only four methods.The efficiency of estimation of some of the methods decreased as organic phosphorus content of the soil increased. This, together with several other observations, suggests that the organic phosphorus of soils cannot be measured quantitatively at present. Several of the comparisons between methods, however, will be useful for further qualitative work.

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