Regeneration of Yeast Protoplasts prepared by Snail Enzyme

Nature ◽  
1966 ◽  
Vol 210 (5038) ◽  
pp. 845-845 ◽  
Author(s):  
A. SVOBODA ◽  
O. NEČAS
Keyword(s):  
Yeast Protoplasts ◽  
Snail Enzyme

EFFECT OF ALKYL BENZENE SULPHONATE ON YEAST METABOLISM

1963 ◽  
Vol 9 (1) ◽  
pp. 117-127
Author(s):  
E. R. Blakley
Keyword(s):  
Cell Wall ◽  
Candida Utilis ◽  
Complete Inhibition ◽  
Enzymatic Reactions ◽  
Alkyl Benzene ◽  
Intact Cells ◽  
Yeast Metabolism ◽  
Ph Values ◽  
Upper Limit ◽  
Yeast Protoplasts

The rate of fermentation of glucose by suspensions of Candida utilis at acid pH values is reduced by alkyl benzene sulphonate in the range 75 to 250 γ/ml. Concentrations of alkyl benzene sulphonate below 75 γ/ml decrease the rate of fermentation of glucose above pH 7 and respiration at all pH values. An upper limit of 70 to 90% inhibition of fermentation or respiration is obtained at concentrations of alkyl benzene sulphonate above 250 γ/ml, except at pH 4.2 where complete inhibition is obtained. The effect of alkyl benzene sulphonate on the fermentation of glucose by yeast protoplasts is similar to the effect observed for intact yeasts. Some enzymatic reactions of cell-free extracts are inhibited by concentrations of alkyl benzene sulphonate lower than that required to affect fermentation by intact cells. The enzyme components of the cell-free preparation appear to vary in their sensitivity to the surfactant. The results support the view that the surfactant in the micellar form disrupts the cell wall of the yeast, and unassociated molecules inactivate some enzymes vital for the metabolism of the cell.

Transformation of protoplasted yeast cells is directly associated with cell fusion

1984 ◽  
Vol 4 (4) ◽  
pp. 771-778
Author(s):  
S Harashima ◽  
A Takagi ◽  
Y Oshima
Keyword(s):  
Cell Fusion ◽  
Mating Type ◽  
Nuclear Fusion ◽  
Yeast Cells ◽  
Nuclear Markers ◽  
Type Locus ◽  
Mating Type Locus ◽  
Yeast Protoplasts ◽  
Yeast Plasmids ◽  
Binominal Distribution

The frequency of cell fusion during transformation of yeast protoplasts with various yeast plasmids with a chromosome replicon (YRp or YCp) or 2 mu DNA (YEp) was estimated by two methods. In one method, a mixture of protoplasts of two haploid strains with identical mating type and complementary auxotrophic nuclear markers with or without cytoplasmic markers was transformed. When the number of various phenotypic classes of transformants for the nuclear markers was analyzed by equations derived from binominal distribution theory, the frequency of nuclear fusion among the transformants was 42 to 100% in transformations with the YRp or YCp plasmids and 28 to 39% with the YEp plasmids. In another method, a haploid bearing the sir mutation, which allows a diploid (or polyploid) homozygous for the MAT (mating type) locus to sporulate by the expression of the silent mating-type loci HML and HMR, was transformed with the plasmids. Sporulation ability was found in 43 to 95% of the transformants with the YRp or YCp plasmids, and 26 to 31% of the YEp transformants. When cytoplasmic mixing was included with the nuclear fusion, 96 to 100% of the transformants were found to be cell fusants. Based upon these observations, we concluded that transformation of yeast protoplasts is directly associated with cell fusion.

scholarly journals INHIBITION BY ANTIBIOTICS OF THE GROWTH OF BACTERIAL AND YEAST PROTOPLASTS

1962 ◽  
Vol 84 (3) ◽  
pp. 508-512 ◽  
Author(s):  
Gerald D. Shockman ◽  
J. Oliver Lampen
Keyword(s):  
Yeast Protoplasts

Fixation of Yeast Protoplasts for Electron Microscopy

Nature ◽  
1961 ◽  
Vol 191 (4792) ◽  
pp. 1022-1023 ◽  
Author(s):  
P. F. ELBERS
Keyword(s):  
Electron Microscopy ◽  
Yeast Protoplasts

Absence of glucose-stimulated Transport in yeast protoplasts

1985 ◽  
Vol 30 (2) ◽  
pp. 110-116 ◽  
Author(s):  
A. Kotyk ◽  
D. Michaljamičová ◽  
R. Stružinský ◽  
L. M. Baryshnikova ◽  
H. Sychrová
Keyword(s):  
Yeast Protoplasts
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