Biological Properties and Radiosensitivity of Tumours: Determination of the Cell-Cycle and Time of Synthesis of Deoxyribonucleic Acid Using Tritiated Thymidine and Autoradiography

Over a 24-h period, groups of mice were given a single injection of colchicine (to collect blocked metaphases) and tritiated thymidine (to label nuclei synthesizing deoxyribonucleic acid). Epithelial nuclei in the oesophagus, trachea and ureter were examined and counted in paraffin sections: the duration of deoxyribonucleic acid synthesis (Ts) was calculated from the numbers of blocked metaphases and labelled nuclei, the duration of the post-synthetic gap (TG2) was estimated from the proportion of blocked mataphases labelled, and the cell cycle time (Tc) was calculated from Ts and the proportion of nuclei labelled. In each epithelium the different layers seen by light microscopy were analysed separately. Ts was probably the same for the basal and superficial cells in the trachea (about 8 h), and was probably the same for the basal, intermediate and superficial cells in the ureter (about 5 h). In the oesophagus Ts was 8.5 h. TG2 was probably the same for the basal and superficial cells in the trachea (3.6 h), and probably the same for the basal, intermediate and superficial cells in the ureter (about 4.6 h). In the oesophagus TG2 was 2.8 h. Tc was about 380 h (basal cells) and 1400 h (superficial cells) in the trachea, and about 8000 h (basal and intermediate cells) and 2700 h (superficial cells) in the ureter. In the oesophagus Tc was 41 h.

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MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v3i1.7838 ◽

2011 ◽

Vol 3 (1) ◽

Author(s):

Lies Indah Sutiknowati

Keyword(s):

Oil Spill ◽

Deoxyribonucleic Acid ◽

Denaturing Gradient Gel Electrophoresis ◽

Gas Chromatography Mass Spectrometry ◽

Oil Degradation ◽

Sequence Determination ◽

Blast Search ◽

Degrading Bacteria ◽

Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

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LC–MS/MS Determination of Apigenin in Rat Plasma and Application to Pharmacokinetic Study

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200807113144 ◽

2020 ◽

Vol 21 ◽

Author(s):

Shixing Zhu ◽

Jiayuan Zhang ◽

Zhihua Lv ◽

Mingming Yu

Keyword(s):

Formic Acid ◽

Pharmacokinetic Study ◽

Ammonium Acetate ◽

Rat Plasma ◽

Biological Properties ◽

Plasma Samples ◽

Supply Rate ◽

Natural Plant ◽

Ammonium Acetate Buffer

Background: Apigenin, a natural plant flavone, has been shown to possess a variety of biological properties. Objective: In this report, a highly selective and sensitive LC-MS/MS method was developed and validated for the determination of apigenin in rat plasma. Methods: Analysts were separated on the HSS T3 column (1.8 μm 2.1×100 mm) using acetonitrile and 0.1% formic acid in 2 mM ammonium acetate buffer at a supply rate of 0.200 mL/min as eluent in gradient model. Results: Plasma samples were treated by protein precipitation using acetonitrile for the recovery ranging from 86.5% to 90.1% for apigenin. The calibration curves followed linearity in the concentration range of 0.50-500 ng/mL. The inter-day and intra-day precisions at different QC levels within 13.1% and the accuracies ranged from -10.6% to 8.6%. Conclusion: The assay has been successfully applied to the pharmacokinetic study of apigenin in rats.

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PDO Rotonda’s Red Eggplant Extract: In vitro Determination of Biological Properties and Minerals Bioaccessibility

Current Nutrition & Food Science ◽

10.2174/1573401314666180622110952 ◽

2020 ◽

Vol 16 (1) ◽

pp. 65-74

Author(s):

Ortensia Ilaria Parisi ◽

Mariarosa Ruffo ◽

Fabio Amone ◽

Rocco Malivindi ◽

Domenico Gorgoglione ◽

...

Keyword(s):

Control Sample ◽

Specific Area ◽

Biological Properties ◽

Ammonium Molybdate ◽

Antihypertensive Activity ◽

Protected Designation Of Origin ◽

The Family ◽

Oil Red O Staining

Background: The Rotonda’s Red Eggplant belongs to the family of Solanum aethiopicum and it is cultivated in a specific area of Potenza (Basilicata, South of Italy) including villages of Rotonda, Viggianello, Castelluccio Superiore and Castelluccio Inferiore. The Red Eggplant cultivated in this area has gained the PDO, “Protected Designation of Origin”. Objective: The aim of this research was to evaluate the use of PDO Rotonda’s Red Eggplant extract as a possible nutraceutical supplement. The antioxidant, antihypertensive, hypoglycemic, and hypolipidemic properties were in vitro evaluated. Methods: The antioxidant activity was investigated by evaluating the scavenging properties against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals and by performing the Ammonium Molybdate and Folin-Ciocalteu assay. The hypoglycemic and antihypertensive activity was studied by evaluating the α-Amylase, α-Glucosidase and Angiotensin Converting Enzyme, respectively, inhibiting activity. In order to evaluate the hypolipidemic activity, the pancreatic lipase inhibiting property was determined and Oil Red O staining assay was performed. Finally, to evaluate the possible use of this extract as a minerals supplement, Selenium, Potassium and Chrome bioaccessibility was studied. Results: The obtained results underline the good antioxidant, hypoglycemic, antihypertensive and hypolipidemic in vitro properties of the PDO Rotonda’s Red Eggplant extract. Moreover, the obtained data show a higher minerals bioaccessibility and this higher value could be ascribable to the natural phytocomplex of PDO Rotonda’s Red Eggplant, which increases the minerals bioaccessibility if compare it with a control sample. Conclusion: The obtained results show that PDO Rotonda’s Red Eggplant extract, might be used as a possible nutraceutical supplement, along with traditional therapies, both for its biological properties and for its minerals bioaccessibility value.

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Development and Pharmacokinetics Study of Antifungal Peptide Nanoliposomes by Liquid Chromatography-tandem Mass Spectrometry

Current Pharmaceutical Analysis ◽

10.2174/1573412914666180307155328 ◽

2019 ◽

Vol 15 (4) ◽

pp. 312-318

Author(s):

Shuoye Yang

Keyword(s):

Mass Spectrometry ◽

Biological Properties ◽

Pharmacokinetic Property ◽

Antifungal Peptide ◽

Simple Liquid ◽

Physico Chemical ◽

Liquid Chromatography Tandem Mass ◽

Pegylated Lipids

Background: The therapeutic ability and application of antifungal peptide (APs) are limited by their physico-chemical and biological properties, the nano-liposomal encapsulation would improve the in vivo circulation and stability. </P><P> Objective: To develop a long-circulating liposomal delivery systems encapsulated APs-CGA-N12 with PEGylated lipids and cholesterol, and investigated through in vivo pharmacokinetics. Methods: The liposomes were prepared and characterized, a rapid and simple liquid chromatographytandem mass spectrometry (LC-MS/MS) assay was developed for the determination of antifungal peptide in vivo, the pharmacokinetic characteristics of APs liposomes were evaluated in rats. Results: Liposomes had a large, unilamellar structure, particle size and Zeta potential ranged from 160 to 185 nm and -0.55 to 1.1 mV, respectively. The results indicated that the plasma concentration of peptides in reference solutions rapidly declined after intravenous administration, whereas the liposomeencapsulated ones showed slower elimination. The AUC(0-∞) was increased by 3.0-fold in liposomes in comparison with standard solution (20 mg·kg-1), the half-life (T1/2) was 1.6- and 1.5-fold higher compared to the reference groups of 20 and 40 mg·kg-1, respectively. Conclusion: Therefore, it could be concluded that liposomal encapsulation effectively improved the bioavailability and pharmacokinetic property of antifungal peptides.

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