Prevention of Immunological Tolerance to Shigella Antigens in New-born Mice by Immune Spleen Cell Extracts

Nature ◽  
1965 ◽  
Vol 205 (4977) ◽  
pp. 1231-1232
Author(s):  
HERMAN FRIEDMAN
Keyword(s):  
Spleen Cell ◽  
Immunological Tolerance ◽  
New Born ◽  
Cell Extracts ◽  
Immune Spleen Cell

Immune Spleen Cell-Mediated Protection Against Fatal Hantaan Virus Infection in Infant Mice

1985 ◽  
Vol 151 (4) ◽  
pp. 691-697 ◽  
Author(s):  
T. Nakamura ◽  
R. Yanagihara ◽  
C. J. Gibbs ◽  
D. Carleton Gajdusek
Keyword(s):  
Virus Infection ◽  
Spleen Cell ◽  
Hantaan Virus ◽  
Immune Spleen Cell

scholarly journals Cellular interactions in immune regulation. Hapten-specific suppression by non-T cells and T cell-mediated reversal of suppression

1981 ◽  
Vol 154 (4) ◽  
pp. 1188-1200 ◽  
Author(s):  
RH DeKruyff ◽  
BG Simonson ◽  
GW Siskind
Keyword(s):  
T Cells ◽  
T Cell ◽  
Spleen Cell ◽  
Cell Population ◽  
Spleen Cells ◽  
Suppressor Activity ◽  
Mixed Cell ◽  
Normal Spleen ◽  
Immune Spleen Cell

The ability of lymphoid cells from immunized animals to regulate the response of naive B ceils to the immunizing hapten was studied. Mice were immunized with trinitrophenylated (TNP) bovine gamma globulin (BGG) in complete Freund's adjuvant, and their spleen cells were examined in vivo and in vitro for the presence of specific inhibitory activity. This activity was found to peak 1 wk after immunization, was active against TNP on both T-dependent (BGG) and T-independent (Ficoll and polyacrylamide beads) carriers, and was demonstrable both by mixed cell transfers and mixed cell culture experiments. In in vitro studies, it was shown that the inhibition of the response to TNP- polyacrylamide beads by immune spleen cells was mediated by a non-T cell, possibly a B cell, because the suppressor activity was enriched in a purified B cell preparation. A role for macrophages was not formally ruled out. A specific suppressor factor was produced in vitro by immune spleen cells cultured in the absence of antigen. The suppressor activity was modulated by T .cells because elimination of T cells from the normal spleen cell population decreased suppression; elimination of T cells from the immune spleen cell population did not effect suppression, but elimination of T cells from both the normal and immune spleen cell populations allowed the expression of marked specific suppression. Thus, T cells present in the normal spleen cell population augment the degree of suppression, whereas T cells present in the immune spleen cell population decrease the degree of suppression; that is, T cells present in the immune spleen cell population had the ability to specifically abrogate suppression ("abrosuppression") in a T-independent immune response. It is proposed that the response to a T- independent antigen is regulated by specific suppressor activity generated by a non-T cell and augmented by the interaction of this cell with a T cell. The suppressor activity can be blocked by a specific abrosuppressor T cell. It is suggested that, because suppressor activity appears dominant in the naive state of the immune system, the induction of specific abrosuppressor activity may be essential if an immune response is to take place.

Trypanosoma lewisi: Immune spleen cell transfer in rats

1972 ◽  
Vol 32 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Charles L. Greenblatt ◽  
Dan T. Spira ◽  
Esther Tyroler
Keyword(s):  
Spleen Cell ◽  
Cell Transfer ◽  
Trypanosoma Lewisi ◽  
Immune Spleen Cell

Immunological Tolerance induced by Cyclophosphamide assayed by Plaque Spleen Cell Method

Nature ◽  
1967 ◽  
Vol 213 (5075) ◽  
pp. 498-499 ◽  
Author(s):  
ALAN C. AISENBERG ◽  
BARBARA WILKES
Keyword(s):  
Spleen Cell ◽  
Immunological Tolerance ◽  
Cell Method

scholarly journals PRODUCTION OF IMMUNOLOGICAL TOLERANCE IN MICE AFTER REPEATED INJECTIONS OF DISRUPTED SPLEEN CELLS

1963 ◽  
Vol 118 (5) ◽  
pp. 743-758 ◽  
Author(s):  
C. Martinez ◽  
J. M. Smith ◽  
M. Blaese ◽  
R. A. Good
Keyword(s):  
Spleen Cell ◽  
Neonatal Period ◽  
Adult Life ◽  
Immunological Tolerance ◽  
Mammary Adenocarcinoma ◽  
Repeated Injection ◽  
Spleen Cells ◽  
Tolerant Animal ◽  
Male Skin ◽  
Tolerant State

1. Tolerance of male skin isografts has been regularly produced in female mice of the C57B1 strain sublines 1, 4, and 6 during adult life by repeated injection of completely disrupted spleen cells derived from male donors. The tolerant state is long-lasting since such grafts have remained in place more than 9 months. 2. Prolonged survival of homotransplants of skin has regularly been produced in DBA/2 mice during adult life by repeated injections of completely disrupted spleen cells from Balb/C donors. When injections of disrupted spleen cell material are continued over a sufficiently long period, permanent acceptance of the skin homografts may be obtained between these strains. 3. Immunological tolerance across even the strong H-2 histocompatibility barrier was obtained in the neonatal period and during adult life by repeated injection of disrupted spleen cell preparations. The tolerant state has been revealed by both mammary adenocarcinoma and skin homografting across this strong histocompatibility barrier. 4. In contradistinction to the tolerant state produced by injection of intact spleen cells in neonatal animals or during adult life or that produced by parabiotic union, the tolerance produced by repeated injection of disrupted spleen cell preparations cannot be transferred to syngenic neonatal mice with spleen cells of the tolerant animal. 5. The implications of these findings in transplantation biology and in consideration of the basic nature of tolerance are discussed.

Ultrastructure and Staining of Developing Elastic Tissue

1971 ◽  
Vol 29 ◽  
pp. 244-245
Author(s):  
E. N. Albert
Keyword(s):  
Fine Structure ◽  
Phosphotungstic Acid ◽  
Staining Method ◽  
Rat Aorta ◽  
Uranyl Acetate ◽  
The Other ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Lead Citrate ◽  
New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

Sign in / Sign up

Export Citation Format

Share Document