Hydrocortisone and the Apparent Induction Period for Antibody Formation in vitro

Nature ◽  
1964 ◽  
Vol 202 (4933) ◽  
pp. 712-712 ◽  
Author(s):  
W. J. HALLIDAY ◽  
JUSTINE S. GARVEY
Keyword(s):  
Induction Period ◽  
Antibody Formation ◽  
Antibody Formation In Vitro

scholarly journals GAMMA GLOBULIN AND ANTIBODY FORMATION IN VITRO

1960 ◽  
Vol 112 (2) ◽  
pp. 279-292 ◽  
Author(s):  
G. J. Thorbecke
Keyword(s):  
Lymph Nodes ◽  
Antibody Formation ◽  
Gamma Globulin ◽  
Spleen Cells ◽  
Mesenteric Lymph Nodes ◽  
Normal Spleen ◽  
Peripheral Lymph ◽  
Antibody Formation In Vitro ◽  
Newborn Animals

Gamma globulin formation in vitro by various tissues was studied using the incorporation of C14-L-lysine into a protein precipitable by a specific anti rabbit gamma globulin serum prepared in a sheep. It was demonstrated that the rate of gamma globulin formation in similar numbers of spleen cells is much higher if taken from an immunized rabbit at the height of antibody formation than that in normal spleen cells. Besides spleen, other tissues shown to form gamma globulin in normal adult rabbits were: peripheral lymph nodes, bone marrow, lung, mesenteric lymph nodes, appendix, and thymus. In tissues from newborn rabbits gamma globulin formation could not be demonstrated. Rabbits 1 week old already showed a beginning of significant gamma globulin formation in appendix tissue, followed approximately 3 weeks later by gamma globulin synthesis in spleen and thymus. Histological observations on these tissues were described and correlated with findings on gamma globulin formation. In the discussion an attempt has been made to relate these observations on newborn and immature rabbits to those available in the literature on antibody formation in newborn animals.

scholarly journals GAMMA GLOBULIN AND ANTIBODY FORMATION IN VITRO

1962 ◽  
Vol 116 (3) ◽  
pp. 295-310 ◽  
Author(s):  
G. J. Thorbecke ◽  
R. M. Asofsky ◽  
G. M. Hochwald ◽  
G. W. Siskind
Keyword(s):  
Bone Marrow ◽  
Antibody Production ◽  
Antibody Formation ◽  
Gamma Globulin ◽  
Short Interval ◽  
Secondary Response ◽  
White Pulp ◽  
Booster Injection ◽  
Antibody Formation In Vitro

Antibody formation in vitro by red and white pulp of the spleen and by bone marrow tissue was studied at various days after an intravenous booster injection of soluble antigens such as ovalbumin and bovine gamma globulin (BGG). When the booster injection of antigen was given early (10 days) after an intravenous primary injection, high antibody formation could be demonstrated in the spleen primarily 2 to 3 days after the injection, but much less afterwards. When the booster injection was given later (1 month) after the primary, the antibody production by the spleen lasted longer and higher serum titers were obtained. The bone marrow formed antibody in both cases but, particularly with the short interval between injections, its response was delayed as compared to the spleen. It was also shown that during antibody formation the production of gamma globulin in vitro was enhanced. Histologically the antibody production was always correlated to immature plasma cell proliferation, located at the border of red and white pulp and in the red pulp of the spleen. When endotoxin had been injected at the time of a primary BGG injection, and a second antigen injection was given 5 to 10 days later, a booster response could be elicited which was sometimes limited to the white pulp on day 1, and on day 2 was divided between "red" and "white" pulp. The response induced at day 10, at the peak of secondary nodule proliferation, lasted very long and was accompanied by an enormous plasma cellular proliferation in and around the periarteriolar lymphoid areas of the spleen. The possible importance of the secondary nodules of the white pulp in the preparation for a secondary response is discussed.

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