Effect of Adrenocorticotrophic Hormone and Cortisone on the Uptake of Tritiated Thymidine by Regenerating Liver Tissue in the White Rat

Nature ◽  
1964 ◽  
Vol 201 (4922) ◽  
pp. 930-931 ◽  
Author(s):  
JAN W. GUZEK
Keyword(s):  
Liver Tissue ◽  
Tritiated Thymidine ◽  
Adrenocorticotrophic Hormone ◽  
Regenerating Liver

Evidence that interaction of hepatocytes with the collecting (hepatic) veins triggers position-specific transcription of the glutamine synthetase and ornithine aminotransferase genes in the mouse liver

1991 ◽  
Vol 11 (12) ◽  
pp. 6050-6058
Author(s):  
F C Kuo ◽  
J E Darnell
Keyword(s):  
Blood Flow ◽  
Carbon Tetrachloride ◽  
Glutamine Synthetase ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Hepatic Veins ◽  
Surgical Removal ◽  
Ornithine Aminotransferase ◽  
Regenerating Liver ◽  
Portal Veins

We previously demonstrated that glutamine synthetase (GS) and ornithine aminotransferase (OAT) mRNAs are expressed in the mouse liver acinus preferentially in pericentral hepatocytes, that is, those immediately surrounding terminal central veins (A.L. Bennett, K.E. Paulson, R.E. Miller, and J.E. Darnell, Jr., J. Cell Biol. 105:1073-1085, 1987, and F.C. Kuo, W.L. Hwu, D. Valle, and J.E. Darnell, Jr., Proc. Natl. Acad. Sci. USA, in press). We now show that hepatocytes surrounding large collecting hepatic veins but not portal veins also express these two mRNAs. The pericentral hepatocytes are the most distal hepatocytes with respect to acinar blood flow, whereas this is not necessarily the case for hepatocytes next to the large collecting hepatic veins. This result implies that it is contact with some hepatic venous element which signals positional expression. In an effort to induce conditions that change relationships between hepatocytes and blood vessels, regenerating liver was studied. After surgical removal of two-thirds or more of the liver, there was no noticeable change in GS or OAT expression in the remaining liver tissue during regeneration. However, treatment with carbon tetrachloride (CCl4), which specifically kills pericentral hepatocytes, completely removed GS- and OAT-containing cells and promptly halted hepatic transcription of GS. Repair of CCl4 damage is associated with invasion of inflammatory and scavenging cells, which remove dead hepatocytes to allow regrowth. Only when hepatocytes resumed contact with pericentral veins were the pretreatment levels of OAT and GS mRNA and high levels of GS transcription restored.

Somatostatin promotes accumulation of phospholipids in regenerating liver tissue of rats

1991 ◽  
Vol 11 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Cedomila Milin ◽  
Biserka Radosevic-Stasic ◽  
Madena Kirigin ◽  
Visnja Hinic ◽  
Daniel Rukavina ◽  
...  
Keyword(s):  
Growth Factors ◽  
Rapid Growth ◽  
Liver Tissue ◽  
Specific Effect ◽  
Protein Accumulation ◽  
Regenerating Liver ◽  
Total Lipids ◽  
Tissue Contents

Effects of somatostatin (SOM) on tissue contents of proteins, total lipids and phospholipids were investigated in regenerating and intact liver tissue of Y-59 rats. Whereas SOM inhibited protein accumulation in regenerating liver, the hormone evoked and increase in total lipids, and specially in phosphatidylcholine, phosphatidylethnolamine, phosphatidylserine (PS) and phosphatidylinositol (PI). Since the same effects were not seen when intact liver was analyzed, it is assumed that SOM acts primarily on tissue stimulated to rapid growth. The increase of PS+PI fractions indicates a specific effect of SOM on the metabolism of phosphatidylinositides. Such an effect might result from the interference of the hormone with the action of growth factors that accelerate phosphatidylinositol breakdown.

486 Stellate-Like Mesenchymal Stem Cells Cultured from Omentum-Induced Regenerating Liver Tissue

2009 ◽  
Vol 136 (5) ◽  
pp. A-78
Author(s):  
Nishit Pancholi ◽  
Mark Kraus ◽  
Jilpa Patel ◽  
Krishnamurthy P. Gudehithlu ◽  
Perianna Sethupathi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liver Tissue ◽  
Regenerating Liver ◽  
Cells Cultured

scholarly journals Evidence that interaction of hepatocytes with the collecting (hepatic) veins triggers position-specific transcription of the glutamine synthetase and ornithine aminotransferase genes in the mouse liver.

1991 ◽  
Vol 11 (12) ◽  
pp. 6050-6058 ◽  
Author(s):  
F C Kuo ◽  
J E Darnell
Keyword(s):  
Blood Flow ◽  
Carbon Tetrachloride ◽  
Glutamine Synthetase ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Hepatic Veins ◽  
Surgical Removal ◽  
Ornithine Aminotransferase ◽  
Regenerating Liver ◽  
Portal Veins

We previously demonstrated that glutamine synthetase (GS) and ornithine aminotransferase (OAT) mRNAs are expressed in the mouse liver acinus preferentially in pericentral hepatocytes, that is, those immediately surrounding terminal central veins (A.L. Bennett, K.E. Paulson, R.E. Miller, and J.E. Darnell, Jr., J. Cell Biol. 105:1073-1085, 1987, and F.C. Kuo, W.L. Hwu, D. Valle, and J.E. Darnell, Jr., Proc. Natl. Acad. Sci. USA, in press). We now show that hepatocytes surrounding large collecting hepatic veins but not portal veins also express these two mRNAs. The pericentral hepatocytes are the most distal hepatocytes with respect to acinar blood flow, whereas this is not necessarily the case for hepatocytes next to the large collecting hepatic veins. This result implies that it is contact with some hepatic venous element which signals positional expression. In an effort to induce conditions that change relationships between hepatocytes and blood vessels, regenerating liver was studied. After surgical removal of two-thirds or more of the liver, there was no noticeable change in GS or OAT expression in the remaining liver tissue during regeneration. However, treatment with carbon tetrachloride (CCl4), which specifically kills pericentral hepatocytes, completely removed GS- and OAT-containing cells and promptly halted hepatic transcription of GS. Repair of CCl4 damage is associated with invasion of inflammatory and scavenging cells, which remove dead hepatocytes to allow regrowth. Only when hepatocytes resumed contact with pericentral veins were the pretreatment levels of OAT and GS mRNA and high levels of GS transcription restored.

Tritiated thymidine autoradiography in the regenerating liver of the dog

1965 ◽  
Vol 5 (2) ◽  
pp. 72-78 ◽  
Author(s):  
Bernard Sigel ◽  
Giselle Pechet ◽  
Marcos Y. Que ◽  
Richard A. MacDonald
Keyword(s):  
Tritiated Thymidine ◽  
Regenerating Liver ◽  
Thymidine Autoradiography

scholarly journals Changes in mitochondrial ultrastructure and malondialdehyde level in regenerating liver tissue in rats

2010 ◽  
Vol 18 (22) ◽  
pp. 2302
Author(s):  
Yun-Heng Zhou ◽  
Guang-Ya Cao ◽  
Bin Yuan ◽  
Bing-Hua Jiao ◽  
Ming-Yong Miao
Keyword(s):  
Liver Tissue ◽  
Regenerating Liver ◽  
Mitochondrial Ultrastructure

MALDI-imaging: a tool to study lipid distribution in liver tissue during regeneration

2010 ◽  
Vol 48 (01) ◽  
Author(s):  
S Zellmer ◽  
A Veloso ◽  
E Astigarraga ◽  
JA Fernández ◽  
R Gebhardt
Keyword(s):  
Liver Tissue ◽  
Maldi Imaging ◽  
Lipid Distribution

Progestation

1967 ◽  
Vol 56 (3_Suppla) ◽  
pp. S7-S45 ◽  
Keyword(s):  
Tritiated Thymidine ◽  
Succinic Dehydrogenase ◽  
Glandular Epithelium ◽  
Synthetic Activity ◽  
Cell Renewal ◽  
Pulse Labelling ◽  
Uterine Receptivity ◽  
Alkaline Phosphatases ◽  
Monocytic Cells ◽  
Acid And Alkaline Phosphatases

ABSTRACT Autoradiographic, enzymic and histologic studies on uteri of pregnant rats were carried out to follow the endometrial modifications which take place during progestation (days L0 – L4) and culminate in the state of uterine receptivity essential for ovum-implantation. Pulse labelling with tritiated thymidine (radioactive DNA precursor) on L0, L1 and L2 revealed a sequence of cell renewal in luminal and glandular epithelium and endometrial stroma. On L3 and L4 stromal cells showed extensive incorporation of tritiated thymidine. This synthetic activity was associated with endometrial preparation for decidualization and was evoked at least in part, by the surge of oestrogen on L3. All layers of the uterine wall were heavily infiltrated on L0 and resembled the site of an acute inflammatory reaction. Subsequently, polymorphonuclear infiltration diminished and monocytic cells predominated. On L3 a spatial arrangement was observed: eosinophiles were concentrated in the basal endometrium and monocytic cells in the subepithelial stroma. A comparison was made between such a shift in migratory cells in the uterus and similar phenomena which occur in inflammatory and immune reactions. Activities of acid and alkaline phosphatases, of ATP-ase and succinic dehydrogenase were low on L0 and L1 during the periods of infiltration, degeneration and regeneration of luminal and glandular epithelium. Enzymic activities increased on the following days, (L3 and L4). Vascular dilation and engorgement and endometrial oedema were observed near the blastocysts on L4. Most blastocysts incorporated tritiated thymidine after 14.00 h on L4, but some showed uptake before loss of the zona which occurs usually between 14.00 and 16.00 h; therefore, it was assumed that the permeability of the zona increases prior to being shed. Activities of succinic dehydrogenase and acid and alkaline phosphatases were demonstrable in blastocysts on L4 while they were still »free« in the uterine lumen.

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