Effects of Alloxan Diabetes and Adrenaline on Concentrations of Free Fatty Acids in Rat Heart and Diaphragm Muscles

Nature ◽  
1963 ◽  
Vol 199 (4891) ◽  
pp. 381-382 ◽  
Author(s):  
P. B. GARLAND ◽  
P. J. RANDLE
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Rat Heart ◽  
Alloxan Diabetes

Regulation of lysophosphatidylcholine-metabolizing enzymes in isolated myocardial cells from rat heart

1985 ◽  
Vol 63 (8) ◽  
pp. 944-951 ◽  
Author(s):  
David L. Severson ◽  
Thea Fletcher
Keyword(s):  
Fatty Acids ◽  
Palmitic Acid ◽  
Free Fatty Acids ◽  
Rat Heart ◽  
Marked Reduction ◽  
Myocardial Cells ◽  
Acyltransferase Activity ◽  
Metabolizing Enzymes ◽  
Enzymatic Pathways ◽  
Lysophospholipase Activity

Enzymatic pathways involved in the metabolism of lysophosphatidylcholine were investigated in rat heart myocardial cells. Acyl CoA-dependent acyltransferase activity was localized in microsomes, and was much greater than lysophospholipase activity in either cytosolic or microsomal fractions. The cytosolic lysophospholipase was more sensitive to inhibition by palmitylcarnitine in comparison to free fatty acids. In contrast, free fatty acids (oleate and palmitate) produced a greater inhibition of the microsomal acyltransferase and lysophospholipase than did palmitylcarnitine. A reduction in the assay pH to 6.5 resulted in an increase in microsomal acyltransferase and cytosolic lysophospholipase activities, but brought about a marked reduction in the microsomal lysophospholipase activity. At pH 6.5, the percentage inhibition of the microsomal acyltransferase by palmitylcarnitine was reduced, whereas the inhibition by palmitic acid was enhanced. The inhibition of the microsomal lysophospholipase by both palmitylcarnitine and palmitic acid was reduced at pH 6.5. With respect to myocardial ischemia, the inhibition of microsomal acyltransferase by free fatty acids and the reduction in microsomal lysophospholipase activity due to acidosis may contribute to the elevation of cellular lysophosphoglycerides which are arrhythmogenic.

scholarly journals EFFECTS OF CORTISOL ON CULTURED RAT HEART CELLS

1973 ◽  
Vol 57 (1) ◽  
pp. 109-116 ◽  
Author(s):  
J. V. Anastasia ◽  
R. L. McCarl
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Oxidation ◽  
Growth Medium ◽  
Rat Heart ◽  
Acid Oxidation ◽  
Heart Cells ◽  
Atp Production ◽  
Rat Heart Cells

This paper reports the determination of the ability of rat heart cells in culture to release [14C]palmitate from its triglyceride and to oxidize this fatty acid and free [14C]palmitate to 14CO2 when the cells are actively beating and when they stop beating after aging in culture. In addition, the levels of glucose, glycogen, and ATP were determined to relate the concentration of these metabolites with beating and with cessation of beating. When young rat heart cells in culture are actively beating, they oxidize free fatty acids at a rate parallel with cellular ATP production. Both fatty acid oxidation and ATP production remain constant while the cells continue to beat. Furthermore, glucose is removed from the growth medium by the cells and stored as glycogen. When cultured cells stop beating, a decrease is seen in their ability to oxidize free fatty acids and to release them from their corresponding triglycerides. Concomitant with decreased fatty acid oxidation is a decrease in cellular levels of ATP until beating ceases. Midway between initiation of cultures and cessation of beating the cells begin to mobilize the stored glycogen. When the growth medium is supplemented with cortisol acetate and given to cultures which have ceased to beat, reinitiation of beating occurs. Furthermore, all decreases previously observed in ATP levels, fatty acid oxidation, and esterase activity are restored.

DEPRESSION OF CONTRACTILITY IN RAT HEART MUSCLE BY FREE FATTY ACIDS DURING HYPOXIA

The Lancet ◽  
1969 ◽  
Vol 294 (7625) ◽  
pp. 825-826 ◽  
Author(s):  
A.H Henderson ◽  
A.S Most ◽  
E.H Sonnenblick
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Heart Muscle ◽  
Rat Heart

scholarly journals The effect of acute alcohol intoxication on some parameters of nitrogenous metabolism in rats with alloxan diabetes

2012 ◽  
Vol 58 (2) ◽  
pp. 220-223
Author(s):  
N.V. Zharkova ◽  
P.P. Potapov ◽  
A.Yu. Stelmach
Keyword(s):  
Fatty Acids ◽  
Uric Acid ◽  
Free Fatty Acids ◽  
Glutamate Dehydrogenase ◽  
Alloxan Diabetes ◽  
Alcohol Intoxication ◽  
Acute Alcohol Intoxication ◽  
Amp Deaminase ◽  
Experimental Animals ◽  
Tyrosine Transaminase

Acute alcohol intoxication in rats with alloxan diabetes is accompanied by the increase of urea and uric acid and by the decrease in free fatty acids in serum. In the liver of experimental animals the increase of activity of glutamate dehydrogenase, AMP deaminase, and tyrosine transaminase was found.

Sign in / Sign up

Export Citation Format

Share Document