Effect of Mycobacterium tuberculosis H37Rv grown in vivo (LH37Rv) on Ehrlich Carcinoma Ascites Cells

Nature ◽  
1963 ◽  
Vol 199 (4900) ◽  
pp. 1308-1309 ◽  
Author(s):  
M. ARTMAN ◽  
A. BEKIERKUNST
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculosis H37rv ◽  
Ehrlich Carcinoma ◽  
Mycobacterium Tuberculosis H37rv

scholarly journals Antituberculosis and Toxicity Assay of ethanolic extract of Mimba Cortex (Azadirachta indica JUSS.)

2017 ◽  
Vol 6 (2) ◽  
pp. 49
Author(s):  
Cut Fatimah ◽  
Erfan Wahyudi ◽  
Ernawati B.
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Acute Toxicity ◽  
Toxicity Test ◽  
Guinea Pigs ◽  
Ethanol Extract ◽  
Tuberculosis H37rv ◽  
Acute Toxicity Test ◽  
Antituberculosis Activity ◽  
Mycobacterium Tuberculosis H37rv

According to WHO has identified so much people with tuberculosis disorder, and includes a disease that causes death. Mycobacterium tuberculosis has been resistant to antituberculosis drugs were used, while the discovery of new synthetic antituberculosis are very slow. Traditionally, mimba cortex has been used to treat cough and bloody sputum. In previous research proved that the ethanol extract of mimba cortex can inhibit the in vitro growth of Mycobacterium tuberculosis. This study was conducted to determine the potential of mimba cortex as antituberculosis in vivo and toxicity test. Antituberculosis potency test in vivo in guinea pigs infected with Mycobacterium tuberculosis H37Rv directly into the bronchi using nebulizer. Then given mimba cortex extract 3 times a day 100 mg/kgBW and 50 mg/kgBW. Isoniazid, Rifampicin and Ethambutol used as a comparison. Antituberculosis assessment examination conducted by Mycobacterium tuberculosis on bronchial fluid speciments were taken every two weeks and tested in culture with Lowenstein-Jensen method. Acute toxicity test conducted on mice, the LD50 value calculation and observation of liver, kidney, and lung histopathology. The result of research showed that the ethanol extract of mimba cortex have antituberculosis activity in guinea pigs which has infected with Mycobacterium tuberculosis H37Rv, 3 times daily dosing of 100 mg/kgBW for 6 weeks, showed that bacterium from +3 to negative, and 3 times daily dosing of 50mg/kgBW showed that bacterium from +3 to +1. Acute toxicity test results showed LD50 11.85 ± 0.571. That is including mild toxic category.Keywords: mimba cortex, antituberculosis activity, acute toxicity

scholarly journals Which aminoglycoside or fluoroquinolone is more active against Mycobacterium tuberculosis in mice?

1997 ◽  
Vol 41 (3) ◽  
pp. 607-610 ◽  
Author(s):  
N Lounis ◽  
B Ji ◽  
C Truffot-Pernot ◽  
J Grosset
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Survival Rate ◽  
Tuberculosis H37rv ◽  
Lung Lesions ◽  
Mycobacterium Tuberculosis H37rv ◽  
Tuberculosis Model

To identify the most active aminoglycoside or fluoroquinolone for the treatment of tuberculosis, the in vivo activities of four different aminoglycosides and three different fluoroquinolones were compared with that of isoniazid (INH) in a murine tuberculosis model. Mice were each inoculated intravenously with 2.3 x 10(7) CFU of Mycobacterium tuberculosis H37Rv. Treatment began the next day (D1) after inoculation and continued for 4 weeks, at the frequency of six times weekly with one of the following regimens: INH, 25 mg/kg; ofloxacin, 200 mg/kg; levofloxacin, 100 or 200 mg/kg; sparfloxacin (SPFX), 50 mg/kg; and streptomycin, kanamycin, amikacin (AMIKA), and isepamicin, all at 200 mg/kg. The dosages of the treatments were presumably equivalent to their clinically tolerated dosages. The severity of infection and effectiveness of the treatment were assessed by the survival rate, spleen weights, gross lung lesions, and the numbers of CFU in the spleens. The results indicate that INH is more bactericidal than any of the aminoglycosides or fluoroquinolones tested, that AMIKA is the most active aminoglycoside, and that SPFX at 50 mg/kg is far more bactericidal than the treatment with other fluoroquinolones.

scholarly journals Molecular characterization of secretory proteins Rv3619c and Rv3620c from Mycobacterium tuberculosis H37Rv

FEBS Journal ◽  
2010 ◽  
Vol 278 (2) ◽  
pp. 341-353 ◽  
Author(s):  
Anjum Mahmood ◽  
Shubhra Srivastava ◽  
Sarita Tripathi ◽  
Mairaj Ahmed Ansari ◽  
Mohammad Owais ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Molecular Characterization ◽  
Tuberculosis H37rv ◽  
Secretory Proteins ◽  
Mycobacterium Tuberculosis H37rv
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