Demonstration of Humoral and Cellular Fixed Antibodies against Homologous Tissue Antigens

Nature ◽  
1963 ◽  
Vol 197 (4865) ◽  
pp. 403-404
Author(s):  
F. SCHEIFFARTH ◽  
H. WARNATZ ◽  
W. FRENGER
Keyword(s):  
Tissue Antigens ◽  
Homologous Tissue

scholarly journals SPLEEN CELL PROLIFERATION IN RESPONSE TO HOMOLOGOUS ANTIGENS STUDIED IN CONGENIC RESISTANT STRAINS OF MICE

1966 ◽  
Vol 123 (4) ◽  
pp. 665-671 ◽  
Author(s):  
Richard W. Dutton
Keyword(s):  
Spleen Cell ◽  
Single Gene ◽  
Inbred Strains ◽  
Histocompatibility Antigens ◽  
Inbred Strains Of Mice ◽  
Tissue Antigens ◽  
Resistant Strains ◽  
The Difference ◽  
Homologous Tissue

The proliferative responses obtained when spleen cell suspensions from two different inbred strains of mice were mixed were investigated further using congenic resistant strain pairs. Strong responses were obtained in 9 cases out of 12 where the two strains differed at a single gene locus controlling an H-2 histocompatibility antigen. No responses were obtained where the difference occurred at loci controlling weak histocompatibility antigens. These findings have been taken to provide additional circumstantial evidence that the response represents an in vitro homograft reaction to homologous tissue antigens.

Antibodies to species homologous tissue antigens in the rat

1968 ◽  
Vol 154 (3) ◽  
pp. 204-209 ◽  
Author(s):  
J. H. Boss ◽  
D. Nelken
Keyword(s):  
Tissue Antigens ◽  
Homologous Tissue

Reactive changes in leukocytes in animals sensitized with homologous tissue antigens

1969 ◽  
Vol 68 (3) ◽  
pp. 1048-1051
Author(s):  
M. M. Kapichnikov ◽  
P. P. Sakharov ◽  
G. P. Kudrina
Keyword(s):  
Reactive Changes ◽  
Tissue Antigens ◽  
Homologous Tissue

Glucuronyl 3-sulfate occurs exclusively on distal unmyelinated terminals of selected sensory neurons in the peripheral nervous system

1995 ◽  
Vol 53 ◽  
pp. 958-959
Author(s):  
S.S. Spicer ◽  
B.A. Schulte
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cerebral Cortex ◽  
Peripheral Nervous System ◽  
Sensory Neurons ◽  
Sensory Nerves ◽  
Tissue Antigens ◽  
The Central Nervous System ◽  
The Brain ◽  
Leu 7

Generation of monoclonal antibodies (MAbs) against tissue antigens has yielded several (VC1.1, HNK- 1, L2, 4F4 and anti-leu 7) which recognize the unique sugar epitope, glucuronyl 3-sulfate (Glc A3- SO4). In the central nervous system, these MAbs have demonstrated Glc A3-SO4 at the surface of neurons in the cerebral cortex, the cerebellum, the retina and other widespread regions of the brain.Here we describe the distribution of Glc A3-SO4 in the peripheral nervous system as determined by immunostaining with a MAb (VC 1.1) developed against antigen in the cat visual cortex. Outside the central nervous system, immunoreactivity was observed only in peripheral terminals of selected sensory nerves conducting transduction signals for touch, hearing, balance and taste. On the glassy membrane of the sinus hair in murine nasal skin, just deep to the ringwurt, VC 1.1 delineated an intensely stained, plaque-like area (Fig. 1). This previously unrecognized structure of the nasal vibrissae presumably serves as a tactile end organ and to our knowledge is not demonstrable by means other than its selective immunopositivity with VC1.1 and its appearance as a densely fibrillar area in H&E stained sections.

scholarly journals STUDIES ON THE IMMUNOTHERAPY OF RUNT DISEASE IN RATS

1969 ◽  
Vol 129 (4) ◽  
pp. 647-661 ◽  
Author(s):  
Willys K. Silvers ◽  
R. E. Billingham
Keyword(s):  
Early Stage ◽  
Fatal Outcome ◽  
Dosage Level ◽  
Cell Suspensions ◽  
Necessary Condition ◽  
Target Cells ◽  
Protective Agent ◽  
Cutaneous Manifestations ◽  
Therapeutic Value ◽  
Tissue Antigens

Using rats of the Lewis and BN (Ag-B locus incompatible) isogenic strains, a comparative study has been made of the capacity to prevent or mitigate the development of runt disease with: (a) lymph node cell suspensions from normal adult BN rats, (b) node cells, or (c) serum from donors sensitized against Lewis tissue antigens, or (d) heterologous anti-lymphocyte serum (ALS) raised in rabbits against rat thymocytes. Following a standard intravenous or intraperitoneal inoculation of 20 x 106 Lewis node cells into neonatal BN hosts, there are cutaneous manifestations of runt disease within 11–15 days and death invariably takes place within 20 days. However, complete protection is afforded by administration of a similar number of normal BN node cells via a different vein, or admixed with the otherwise harmful Lewis node cells. However, timing of the administration was crucially important—precedence or delay by as little as 4 hr resulted in a great impairment of protection. When the inoculations of the two cell suspensions were separated by 24 hr, no protection was afforded. These and other observations suggested that a necessary condition for protection of the hosts by unsensitized isologous cells requires that they establish a prompt and intimate confrontation with the homologous target cells. At the same dosage level, suspensions of node cells from sensitized isologous donors were much more effective therapeutically, saving the lives of 92% of treated subjects when administered after a delay of 3 days, and of 19% when the delay was 4 or 5 days. Of the various immunotherapeutic agents studied, daily injections of 0.2 ml of isoantiserum gave the best results, and could totally reverse the course of the disease even when initiated at age 10–13 days and subjects already presented symptoms. ALS, although inferior to isoantiserum at the dosage levels tested, proved to be superior to sensitized isologous cells as a protective agent, since the initiation of daily injections after delays of 6 or 8 days were still effective. The observations that delayed treatments of infant rats with isoantisera or ALS resulted in complete recoveries sustain the thesis that the lesions responsible for the fatal outcome of runt diseases are not inflicted at a very early stage. The efficacy of both isoantisera and ALS as a means of inhibiting the progression of homologous disease also suggests that they may have therapeutic value in situations where this condition is encountered.

scholarly journals Conjugation of Peroxidase from Brassica oleracea gongylodes for Use as a Label- Prospect of a Novel Enzyme Tag for Immunoassay Systems

2017 ◽  
Vol 5 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Premalatha Shetty ◽  
Avila D’Souza ◽  
Geethu CP
Keyword(s):  
Brassica Oleracea ◽  
Temperature Stability ◽  
Egg Yolk ◽  
Bromothymol Blue ◽  
Bromocresol Purple ◽  
Significant Extent ◽  
Periodate Treatment ◽  
Ammonium Sulfate Saturation ◽  
Tissue Antigens

Peroxidase tagged proteins are being used successfully as immune-histological probes for the demonstration of tissue antigens, and in enzyme amplified immunoassay systems for the quantitative determination of soluble and insoluble antigens. The glycoprotein nature of peroxidases can be exploited for conjugation to proteins of interest. Peroxidase extracted from the bulbs of Brassica oleracea gongylodes was salted out at 40-80% ammonium sulfate saturation and activated by treatment with 1-Fluoro-2,4-dinitro benzene (FDNB) and periodate. Treatment with 0.08% FDNB and 12.5mM periodate was optimized for activation of the enzyme. The treated enzyme was found to conjugate successfully to immunoglobulin fractions harvested from egg yolk (IgY), human plasma and goat serum. Enzyme conjugated to IgY fraction showed improvement in its pH stability and temperature stability. The affinity of the enzyme for its substrate phenol did not alter to a significant extent upon activation and conjugation. The conjugates exhibited high affinity towards phenol, bromocresol purple and bromothymol blue in comparison to HRP conjugates prepared using the same protocol. Int. J. Appl. Sci. Biotechnol. Vol 5(1): 59-65

Cross reactions between Plasmodium falciparum and mammalian tissue antigens detected by monoclonal antibodies

1984 ◽  
Vol 78 (1) ◽  
pp. 75-76 ◽  
Author(s):  
G. Daniel Ribeiro ◽  
J. Kalil ◽  
L. Monjour ◽  
C. Alfred ◽  
I. Ploton ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Monoclonal Antibodies ◽  
Mammalian Tissue ◽  
Cross Reactions ◽  
Tissue Antigens
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