Adenosine Triphosphatase Activity of Glycerinated Muscle Fibres in a Mechanical Field

Nature ◽  
1963 ◽  
Vol 197 (4863) ◽  
pp. 184-185 ◽  
Author(s):  
TSUYOSHI OHNISHI ◽  
TOMOKO OHNISHI
Keyword(s):  
Adenosine Triphosphatase ◽  
Muscle Fibres ◽  
Adenosine Triphosphatase Activity ◽  
Mechanical Field ◽  
Glycerinated Muscle

scholarly journals CYTOCHEMICAL LOCALIZATION OF MYOFIBRILLAR ADENOSINE TRIPHOSPHATASE ACTIVITY IN SARCOMERES OF GLYCERINATED MUSCLE BY THE CALCIUM PRECIPITATION METHOD A NOVEL CONTROL IS NEEDED TO DEMONSTRATE ARTIFACTS

1971 ◽  
Vol 19 (2) ◽  
pp. 75-84 ◽  
Author(s):  
BERNARD D. TUNIK
Keyword(s):  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Protein Concentration ◽  
Pectoralis Major Muscle ◽  
Precipitation Method ◽  
Cytochemical Localization ◽  
Adenosine Triphosphatase Activity ◽  
High Protein Concentration ◽  
Glycerinated Muscle ◽  
Calcium Precipitation

The calcium precipitation method was used in an attempt to localize myofibrillar adenosine triphosphatase (ATPase) activity within the sarcomeres of single, unfixed, glycerol-extracted fibers from the pectoralis major muscle of leghorn chicks. ATPase was apparently present in only the A-I overlap region of unshortened fibers, and was apparently present in only the contraction bands of shortened fibers. The results are shown to be self-contradictory in view of the known submicroscopic morphology of the sarcomeres. However, the usual classical controls provided no evidence of artifactual localization. In a novel control, separate solutions of calcium and phosphate were caused to diffuse simultaneously into a fiber. The resulting precipitate was localized in patterns indistinguishable from those obtained from incubation of shortened or unshortened fibers in ATP. These patterns result from the localization of precipitate in regions of high protein concentration. Caution in interpreting the results of cytochemical procedures even though controls provide no evidence of artifactual localization is urged.

scholarly journals Environmental CO2 Stimulation of Mitochondrial Adenosine Triphosphatase Activity

1963 ◽  
Vol 238 (2) ◽  
pp. 836-842
Author(s):  
D.D. Fanestil ◽  
A Baird Hastings ◽  
Theodore A. Mahowald
Keyword(s):  
Adenosine Triphosphatase ◽  
Adenosine Triphosphatase Activity ◽  
Stimulation Of
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