Connexion between Protein Synthesis and Salt Absorption in Plant Cells

Nature ◽  
1962 ◽  
Vol 195 (4845) ◽  
pp. 1014-1014 ◽  
Author(s):  
B. JACOBY ◽  
J. F. SUTCLIFFE
1988 ◽  
Vol 7 (4) ◽  
pp. 279-302 ◽  
Author(s):  
Anne M. Johnson‐Flanagan ◽  
Jas Singh ◽  
Norman P.A. Huner

Nature ◽  
1935 ◽  
Vol 136 (3452) ◽  
pp. 1034-1035 ◽  
Author(s):  
W. J. V. Osterhout
Keyword(s):  

Nature ◽  
1965 ◽  
Vol 205 (4974) ◽  
pp. 921-922 ◽  
Author(s):  
W. H. JYUNG ◽  
S. H. WITTWER ◽  
M. J. BUKOVAC

Nature ◽  
1935 ◽  
Vol 135 (3414) ◽  
pp. 553-555 ◽  
Author(s):  
F. C. STEWARD
Keyword(s):  

1979 ◽  
Vol 34 (12) ◽  
pp. 1195-1198 ◽  
Author(s):  
C. Cocito ◽  
O. Tiboni ◽  
F. Vanlinden ◽  
O. Ciferri

Abstract The light-driven incorporation of amino acids by isolated spinach chloroplasts is inhibited by the M component (VM) and not by the S component (VS) of virginiamycin. This inhibitory effect is partially reversible. In chloroplast extracts, poly(U)-directed polyphenylalanine formation is strongly inhibited by VM and not by VS. The in vivo synergistic effect of VM and VS observed in bacteria and algae, does not occur in isolated chloroplasts and chloroplast extracts.


1987 ◽  
Vol 84 (3) ◽  
pp. 739-743 ◽  
Author(s):  
N. K. Singh ◽  
P. C. LaRosa ◽  
A. K. Handa ◽  
P. M. Hasegawa ◽  
R. A. Bressan

F1000Research ◽  
2013 ◽  
Vol 2 ◽  
pp. 185 ◽  
Author(s):  
Noboru Yamauchi ◽  
Tadashi Gosho ◽  
Satoru Asatuma ◽  
Kiminori Toyooka ◽  
Toru Fujiwara ◽  
...  

In Arabidopsis the borate transporter BOR1, which is located in the plasma membrane, is degraded in the presence of excess boron by an endocytosis-mediated mechanism. A similar mechanism was suggested in rice as excess boron decreased rice borate transporter levels, although in this case whether the decrease was dependent on an increase in degradation or a decrease in protein synthesis was not elucidated. To address whether the borate-dependent degradation mechanism is conserved among plant cells, we analyzed the fate of GFP-tagged BOR1 (BOR1-GFP) in transformed tobacco BY-2 cells. Cells expressing BOR1-GFP displayed GFP fluorescence at the plasma membrane, especially at the membrane between two attached cells. The plasma membrane signal was abolished when cells were incubated in medium with a high concentration of borate (3 to 5 mM). This decrease in BOR1-GFP signal was mediated by a specific degradation of the protein after internalization by endocytosis from the plasma membrane. Pharmacological analysis indicated that the decrease in BOR1-GFP largely depends on the increase in degradation rate and that the degradation was mediated by a tyrosine-motif and the actin cytoskeleton. Tyr mutants of BOR1-GFP, which has been shown to inhibit borate-dependent degradation in Arabidopsis root cells, did not show borate-dependent endocytosis in tobacco BY-2 cells. These findings indicate that the borate-dependent degradation machinery of the borate transporter is conserved among plant species.


HortScience ◽  
1992 ◽  
Vol 27 (12) ◽  
pp. 1261d-1261
Author(s):  
Jody A. Goodrich

This research tested the hypothesis that 60Co ionizing irradiation degrades polysomes to monosomes, a process that reduces growth of Pisum sativum seedlings. Dry and imbibed seeds and 5-day-old seedlings were exposed to 1.8, 3.6, 7.2, 14.4, or 28.8 krad of 60Co irradiation. Immediately after irradiation treatments, dry and imbibed seeds were planted, and later seedlings were harvested and analyzed. Five 1-cm root-tip samples from 5-day-old seedlings were crushed and layered onto 15% to 60% sucrose gradients and centrifuged for 55 min. The samples were processed through an ISCO ultraviolet chart maker. The monosome and polysome weights were read and analyzed. The monosome content was greater in the irradiated 5-day-old seedlings than in seedlings from dry and imbibed seeds. The growth of 5-day-old irradiated seedlings and seedlings from imbibed seeds was less than that of seedlings from dry seeds. The reduced growth of the irradiated seedlings suggests damage to the polysomes. When protein synthesis in plant cells is altered, perhaps through RNA decoding mechanisms, growth may be partially or completely arrested. Using sensitive plants to establish the injurious effects of ionizing irradiation on living organisms can educate and alert society to the detrimental effects of overexposure to irradiation such as that caused by nuclear accidents.


Nature ◽  
1935 ◽  
Vol 136 (3452) ◽  
pp. 1035-1035
Author(s):  
F. C. Steward
Keyword(s):  

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