Quantitative Determination of Metadrenaline and Normetadrenaline in Normal Human Urine

Nature ◽  
1961 ◽  
Vol 191 (4788) ◽  
pp. 599-600 ◽  
Author(s):  
KAORU YOSHINAGA ◽  
CHUICHI ITOH ◽  
NOZOMU ISHIDA ◽  
TATSUO SATO ◽  
YASUO WADA
Keyword(s):  
Quantitative Determination ◽  
Human Urine ◽  
Normal Human

A mixed hard- and soft-modelling approach for the quantitative determination of oxipurines and uric acid in human urine

2006 ◽  
Vol 567 (2) ◽  
pp. 236-244 ◽  
Author(s):  
José Manuel Amigo ◽  
Anna de Juan ◽  
Jordi Coello ◽  
Santiago Maspoch
Keyword(s):  
Uric Acid ◽  
Quantitative Determination ◽  
Human Urine ◽  
Modelling Approach

IMMUNOREACTIVE GROWTH HORMONE IN HUMAN URINE

1972 ◽  
Vol 71 (4) ◽  
pp. 665-676 ◽  
Author(s):  
Kristian F. Hanssen
Keyword(s):  
Molecular Weight ◽  
Growth Hormone ◽  
Linear Relationship ◽  
Human Urine ◽  
Gel Filtration ◽  
Human Growth ◽  
Normal Subjects ◽  
Normal Human ◽  
Radio Immunoassay

ABSTRACT By using a double antibody radio-immunoassay (pre-precipitation technique) for the determination of immunoreactive human growth hormone (IRHGH) in normal human urine concentrated by dialysis and lyophilization, a factor was revealed that displaces 125I-HGH from HGH antibodies. This displacement was neither due to salts nor to glucose; it is suggested that it is due to IRHGH in the urine. A linear relationship between dilution of urine and the measured IRHGH concentration was obtained. Recovery of exogenous HGH was between 70–105%. The recovery of IRHGH from different volumes of urine following dialysis and lyophilization was between 97–110%. Plasma IRHGH and urinary IRHGH was measured simultaneously after HGH injection in a normal subject. A correlation was shown between plasma IRHGH and urinary IRHGH. In 9 normal subjects, the urinary IRHGH ranged from 28–53 ng/24 h. The excretion of urinary IRHGH was increased in acromegaly and was diminished in some, but not in all patients with adult hypopituitarism. The urinary IRHGH was further studied by gel filtration. It was recovered in one peak corresponding to a molecular weight of approximately 20 000 – 30 000. However, in the present work it was not clarified whether the urinary IRHGH represents pituitary HGH excreted in the urine or a metabolite of high molecular weight with retained immunological properties.

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