Comparison of Short-Circuit Current and Net Water Movement in Single Perfused Proximal Tubules of Rat Kidneys

Nature ◽  
1961 ◽  
Vol 191 (4794) ◽  
pp. 1205-1207 ◽  
Author(s):  
ERICH E. WINDHAGER ◽  
GERHARD GIEBISCH
Keyword(s):  
Water Movement ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Proximal Tubules ◽  
Rat Kidneys

OSMOTIC INHIBITION OF THE NATRIFERIC RESPONSE OF THE TOAD URINARY BLADDER TO VASOPRESSIN

1975 ◽  
Vol 67 (1) ◽  
pp. 119-125
Author(s):  
P. J. BENTLEY
Keyword(s):  
Urinary Bladder ◽  
Water Movement ◽  
Short Circuit ◽  
Electrical Potential ◽  
Short Circuit Current ◽  
Toad Bladder ◽  
Toad Urinary Bladder ◽  
Electrical Potential Difference ◽  
Osmotic Gradient

SUMMARY The electrical potential difference and short-circuit current (scc, reflecting active transmural sodium transport) across the toad urinary bladder in vitro was unaffected by the presence of hypo-osmotic solutions bathing the mucosal (urinary) surface, providing that the transmural flow of water was small. Vasopressin increased the scc across the toad bladder (the natriferic response), but this stimulation was considerably reduced in the presence of a hypo-osmotic solution on the mucosal side, conditions under which water transfer across the membrane was also increased. This inhibition of the natriferic response did not depend on the direction of the water movement, for if the osmotic gradient was the opposite way to that which normally occurs, the response to vasopressin was still reduced. The natriferic response to cyclic AMP was also inhibited in the presence of an osmotic gradient. Aldosterone increased the scc and Na+ transport across the toad bladder but this response was not changed when an osmotic gradient was present. The physiological implications of these observations and the possible mechanisms involved are discussed.

Response of active transport of ions and spontaneous water flux to osmotic gradients in gastric mucosa

1975 ◽  
Vol 228 (3) ◽  
pp. 738-741 ◽  
Author(s):  
L Villegas
Keyword(s):  
Gastric Mucosa ◽  
Water Content ◽  
Water Movement ◽  
Short Circuit ◽  
Water Flux ◽  
Short Circuit Current ◽  
Ion Flux ◽  
Bathing Solution ◽  
Cell Water ◽  
Concentration Gradients

The effects of symmetric changes of the mucosal and serosal bathing solution on cell water content, net ion flux, and net water movement were studied in the isolated frog gastric mucosa. Similar to transmucosal concentration gradients that induce water movement and changes in cell water content, symmetric osmolality changes of the bathing solutions also produce changes in these functional parameters. Thus, increments from 165 to 286 mosmol/kg water in the osmolality of both solutions reduce cell water content from 2.37 plus or minus 0.12 to 1.30 plus or minus 0.20 ml/g wt, the net ion flux (acid secretion plus short-circuit current) from 4.83 plus or minus 0.36 to 3.44 plus or minus 0.26 mueq/cm2 per h, and the net water flux from 10.6 plus or minus 1.1 to 2.4 plus or minus 1.2 mul/cm2 per h. These osmotically induced flux changes of water and ions must be considered when osmotic gradients are being used to generate and to evaluate water movement across the gastric mucosa.

scholarly journals Sodium Movement across Single Perfused Proximal Tubules of Rat Kidneys

1964 ◽  
Vol 47 (6) ◽  
pp. 1175-1194 ◽  
Author(s):  
Gerhard Giebisch ◽  
Ruth M. Klose ◽  
Gerhard Malnic ◽  
W. James Sullivan ◽  
Erich E. Windhager
Keyword(s):  
Pressure Gradient ◽  
Osmotic Pressure ◽  
Water Movement ◽  
Rat Kidney ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Colloid Osmotic Pressure ◽  
Concentration Difference ◽  
The Relationship ◽  
Osmotic Pressure Gradient

Using perfusion techniques in single proximal tubule segments of rat kidney, the relationship between net sodium movement and active transport of ions, as measured by the short-circuit method, has been studied. In addition, the role of the colloid-osmotic pressure gradient in proximal transtubular fluid and sodium movement has been considered. Furthermore, the limiting concentration gradient against which sodium movement can occur and the relationship between intratubular sodium concentration and fluid transfer have been investigated. Comparison of the short-circuit current with the reabsorptive movement of sodium ions indicates that this process is largely, perhaps exclusively, active in nature. No measurable contribution of the normally existing colloid-osmotic pressure gradient to transtubular water movement was detected. On the other hand, fluid movement across the proximal tubular epithelium is dependent upon the transtubular sodium gradient and is abolished when a mean concentration difference of 50 mEq/liter is exceeded.

scholarly journals EFFECTS OF CYTOCHALASIN B ON THE RESPONSE OF TOAD URINARY BLADDER TO VASOPRESSIN

1974 ◽  
Vol 63 (3) ◽  
pp. 986-997 ◽  
Author(s):  
Walter L. Davis ◽  
David B. P. Goodman ◽  
Richard J. Schuster ◽  
Howard Rasmussen ◽  
James H. Martin
Keyword(s):  
Urinary Bladder ◽  
Water Permeability ◽  
Morphological Study ◽  
Cytochalasin B ◽  
Water Movement ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Toad Bladder ◽  
Toad Urinary Bladder ◽  
Osmotic Response

A combined physiological and morphological study of the effects of cytochalasin B (CB) on the toad urinary bladder has been carried out. CB inhibits the hydro-osmotic response to vasopressin without altering basal water permeability or diffusion, or the increase in 3H2O diffusion observed after hormone addition. Although CB increases [22Na]-, [36Cl]-, and [14C]urea fluxes, and decreases transepithelial potential, no alteration in basal short-circuit current, the vasopressin-induced increase in this parameter, or [14C]inulin permeability occurs. In the absence of hormone, CB does not markedly alter the structure of the toad bladder. However, in the presence of vasopressin, CB induces the formation of large intracellular vacuoles. These results suggest a possible coupling of solute and water movement across the tissue.

Asymmetrical permeability of the integument of tree frogs (Hylidae)

1977 ◽  
Vol 67 (1) ◽  
pp. 197-204
Author(s):  
T. Yorio ◽  
P. J. Bentley
Keyword(s):  
Water Movement ◽  
Short Circuit ◽  
Tritiated Water ◽  
Short Circuit Current ◽  
Single Species ◽  
Dorsal Side ◽  
Dorsal Skin ◽  
Tree Frogs ◽  
Ventral Skin

The skin from four species of North American tree frogs (Hylidae) was found in vitro, to be remarkably permeable to water in the presence or absence of the neurohypophysial hormone vasotocin. This property was, however, only seen in preparations from the ventral integument; osmotic water movement across the dorsal skin was negligible. The ionic permeability, reflected by the electrical resistance, was also much greater in the ventral skin. Unidirectional fluxes of 22Na, 36Cl, 14C-urea and tritiated water were measured in vitro in a single species, Agalychnis dacnicolor Cope. The ventral skin was 10-20 times more permeable to these molecules than the dorsal skin. The short-circuit current (usually reflecting active transmural Na transport) across the ventral skin was increased by aldosterone and vasotocin but these responses were absent on the dorsal side. The lipid content of the dorsal skin was four times greater than that on the ventral surface.

Water and ion handling in the rat cecum

1990 ◽  
Vol 259 (5) ◽  
pp. G786-G791 ◽  
Author(s):  
E. Escobar ◽  
C. Ibarra ◽  
E. Todisco ◽  
M. Parisi
Keyword(s):  
Permeability Coefficient ◽  
Driving Force ◽  
Water Movement ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Chemical Gradient ◽  
Osmotic Permeability ◽  
Rat Cecum ◽  
Good Agreement

The minute-by-minute net water movement (Jw) in the rat cecum was correlated with the transepithelial potential difference (PD), short-circuit current (Isc), and the unidirectional Na+, Cl-, and Rb+ fluxes, with the following results. 1) Jw was a linear function of the applied hydrostatic or osmotic transepithelial gradients (hydrostatic permeability coefficiency = 0.164 +/- 0.018 cm/s, n = 13; osmotic permeability coefficient = 0.0014 +/- 0.0002 cm/s, n = 6). 2) A fraction of this absorptive Jw (0.17 +/- 0.03 microliter.min-1.cm-2, n = 13) was independent of the presence of any osmotic, hydrostatic, or chemical gradient. 3) This fraction was Na+ dependent, associated with an amiloride-insensitive PD and net Na+ (2.37 +/- 0.68 mu eq.h-1.cm-2, n = 6) and Cl- influxes (3.45 +/- 1.46 mu eq.h-1.cm-2, n = 6), measured under short-circuit conditions. No net Rb+ movement was detected. 4) The absorptive Jw increased when HCO3- was replaced by tris(hydroxymethyl)aminomethane (Tris+) buffer or Cl- by SO4(2-). A good agreement between the observed and the expected Jw (assuming isosmotic reabsorption) was observed in the absence of HCO3-. 5) The presence of an osmotic but not a hydrostatic transepithelial gradient generated a transepithelial PD. These results show that water movement across the rat cecum in vitro is the result of a combination of hydrostatic-, osmotic-, and transport-associated transfers. Concerning this last driving force, the observed results indicate that the transport-related Jw results from the addition of an absorptive Jw, coupled to a nonelectrogenic NaCl entry, plus a secretory Jw probably coupled to HCO3- secretion.

Estimation Methods of Short Circuit Current using Normal PMU Measurements and Field Testing

2013 ◽  
Vol 133 (1) ◽  
pp. 37-44
Author(s):  
Suresh Chand Verma ◽  
Yoshiki Nakachi ◽  
Yoshihiko Wazawa ◽  
Yoko Kosaka ◽  
Takenori Kobayashi ◽  
...  
Keyword(s):  
Short Circuit ◽  
Short Circuit Current ◽  
Field Testing ◽  
Estimation Methods

About the Selection of a Single-Phase Short-Circuit Current on Earth in the Network With Low-Ohm Resistive Grounding of the Neutral

2017 ◽  
pp. 34-41
Author(s):  
Andrei V. MAIOROV ◽  
◽  
Kirill A. OSINTSEV ◽  
Andrei V. SHUNTOV ◽  
◽  
...  
Keyword(s):  
Single Phase ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Selection Of

Optical Absorption Enhancement in Polymer BHJ thin Film Using Ag Nanostructures: A Simulation Study

2020 ◽  
Vol 16 (4) ◽  
pp. 556-567
Author(s):  
Asma Khalil ◽  
Zubair Ahmad ◽  
Farid Touati ◽  
Mohamed Masmoudi
Keyword(s):  
Absorption Spectrum ◽  
Solar Cell ◽  
Methyl Ester ◽  
Butyric Acid ◽  
Organic Solar Cell ◽  
Short Circuit ◽  
Acid Methyl Ester ◽  
Short Circuit Current ◽  
Acid Methyl ◽  
Butyric Acid Methyl Ester

Background: The photo-absorption and light trapping through the different layers of the organic solar cell structures are a growing concern now-a-days as it affects dramatically the overall efficiency of the cells. In fact, selecting the right material combination is a key factor in increasing the efficiency in the layers. In addition to good absorption properties, insertion of nanostructures has been proved in recent researches to affect significantly the light trapping inside the organic solar cell. All these factors are determined to expand the absorption spectrum and tailor it to a wider spectrum. Objective: The purpose of this investigation is to explore the consequence of the incorporation of the Ag nanostructures, with different sizes and structures, on the photo absorption of the organic BHJ thin films. Methods: Through a three-dimensional Maxwell solver software, Lumerical FDTD, a simulation and comparison of the optical absorption of the three famous organic materials blends poly(3- hexylthiophene): phenyl C71 butyric acid methyl ester (P3HT:PCBM), poly[N-9″-heptadecanyl-2,7- carbazole-alt-5,5-(4′,7′-di-2-thienyl-2′,1′,3′-benzothiadiazole)]: phenyl C71 butyric acid methyl ester (PCDTBT:PCBM) and poly[2,6-(4,4-bis-(2-ethylhexyl)-4H-cyclopenta[2,1-b;3,4-b′]dithiophene)-alt- 4,7-(2,1,3-benzothiadiazole)]: phenyl C71 butyric acid methyl ester (PCDPDTBT:PCBM) has been conducted. Furthermore, FDTD simulation study of the incorporation of nanoparticles structures with different sizes, in different locations and concentrations through a bulk heterojunction organic solar cell structure has also been performed. Results: It has been demonstrated that embedding nanostructures in different locations of the cell, specifically in the active layer and the hole transporting layer had a considerable effect of widening the absorption spectrum and increasing the short circuit current. The effect of incorporation the nanostructures in the active layer has been proved to be greater than in the HTL. Furthermore, the comparison results showed that, PCDTBT:PCBM is no more advantageous over P3HT:PCBM and PCPDTBT:PCBM, and P3HT:PCBM took the lead and showed better performance in terms of absorption spectrum and short circuit current value. Conclusion: This work revealed the significant effect of size, location and concentration of the Ag nanostructures while incorporated in the organic solar cell. In fact, embedding nanostructures in the solar cell widen the absorption spectrum and increases the short circuit current, this result has been proven to be significant only when the nanostructures are inserted in the active layer following specific dimensions and structures.

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