Changes in the Pentose–Nucleic Acid Content of Sub-cellular Fractions of the Brain of the Rat during ‘Metrazol’ Convulsions

Nature ◽  
1961 ◽  
Vol 191 (4792) ◽  
pp. 1007-1008 ◽  
Author(s):  
G. P. TALWAR ◽  
B. SADASIVUDU ◽  
V. S. CHITRE
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Metrazol Convulsions ◽  
The Brain

scholarly journals Relative Growth, Nucleic Acid Content and Cell Numbers of the Brain in Octopus Vulgaris (Lamarck)

1970 ◽  
Vol 52 (3) ◽  
pp. 539-552
Author(s):  
A. PACKARD ◽  
V. ALBERGONI
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Fold Increase ◽  
Small Cells ◽  
Nucleic Acid Content ◽  
Octopus Vulgaris ◽  
Homogeneous Population ◽  
Optic Lobes ◽  
Wet Weight ◽  
The Brain

1. The brain of Octopus vulgaris grows allometrically with respect to weight from 12 mg. at the beginning of post-larval life to 1.95 g. at a body weight of 8.5 kg. 2. The two optic lobes contribute 64% to the wet weight total at the beginning of the growth range and 44% at the end. 3. The 150-fo1d increase in brain weight is accompanied by a 40-fold increase in total DNA content, 80-85% of which is in the optic lobes. DNA concentrations of the order of 20 mg./g. wet weight are found in the vertical lobes. 4. An average of 1 mg. DNA is yielded by 1.9x108Octopus sperms and this figure is used to calibrate the nucleic acid content of the optic lobes, and of the suboesophageal and supraoesophageal divisions of the brain in terms of their ‘diploid equivalent’. 5. The ratio between the ‘diploid equivalent’ and the numbers of neurones counted histologically is highest for the suboesophageal division and lowest for the vertical lobes and probably indicates a high glia/neurone index in brain areas with many large cells and low index in those with a homogeneous population of small cells. The brain cells of O. vulgaris do not appear to be polyploid. 6. The RNA/DNA ratio is less than 1.0 in the optic and supraoesophageal lobes, higher than 1.0 in the suboesophageal lobes. The ratio increases during growth, especially in the suboesophageal division.

Application of Ogur & Rosen's Method for the Determination of Nucleic Acid Content of Bacteria

1957 ◽  
Vol 12 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Nobuyasu KAWASAKI ◽  
Ichiro TAKI ◽  
Chiaki WATANABE ◽  
Kiyoshi MATOBA ◽  
Mokichiro NISHIO ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content

scholarly journals Using flow cytometry and light-induced fluorescence technique to characterizethe variability and characteristics of bioaerosols in springtime at Metro Atlanta, Georgia

2018 ◽  
Author(s):  
Arnaldo Negron ◽  
Natasha DeLeon-Rodriguez ◽  
Samantha M. Waters ◽  
Luke D. Ziemba ◽  
Bruce Anderson ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Acid Content ◽  
Fungal Spores ◽  
Epifluorescence Microscopy ◽  
Nucleic Acid Content ◽  
Rain Event ◽  
Bacterial Cells ◽  
Sampling System ◽  
Spores And Pollen

Abstract. The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large volumes of air with a portable wet-walled cyclone bioaerosol sampler. A flow cytometry (FCM) protocol was then developed to quantify and characterize the PBAP populations from the sampler, which were confirmed against epifluorescence microscopy. The sampling system and FCM analysis were used to study PBAP in Atlanta, GA over a two-month period and showed clearly defined populations of DNA-containing particles: Low Nucleic Acid-content particles (bioLNA), High Nucleic Acid-content particles (HNA) being fungal spores and pollen. We find that daily-average springtime PBAP concentration (1 to 5 μm diameter) ranged between 1.4 × 104 and 1.1 × 105 m−3. The BioLNA population dominated PBAP during dry days (72 ± 18 %); HNA dominated the PBAP during humid days and following rain events, where HNA (e.g., wet-ejected fungal spores) comprised up to 92 % of the PBAP number. Concurrent measurements with a Wideband Integrated Bioaerosol Sensor (WIBS-4A) showed that FBAP and total FCM counts are similar; HNA (from FCM) significantly correlated with ABC type FBAP concentrations throughout the sampling period (and for the same particle size range, 1–5 μm diameter). However, the FCM bioLNA population, possibly containing bacterial cells, did not correlate to any FBAP type. The lack of correlation of any WIBS FBAP type with the bioLNA suggest bacterial cells may be more difficult to detect with autofluorescence than previously thought. Ιdentification of bacterial cells even in the FCM (bioLNA population) is challenging, given that the fluorescence level of stained cells at times may be comparable to that seen from abiotic particles. HNA and ABC displayed highest concentration on a humid and warm day after a rain event (4/14), suggesting that both populations correspond to wet-ejected fungal spores. Overall, information from both instruments combined reveals a highly dynamic airborne bioaerosol community over Atlanta, with a considerable presence of fungal spores during humid days, and a bioLNA population dominating bioaerosol community during dry days.

Age-Related Effect of Dietary Nucleotides on Liver Nucleic Acid Content in Rats

1997 ◽  
Vol 41 (5) ◽  
pp. 324-330 ◽  
Author(s):  
Ana T. López-Navarro ◽  
Angel Gil ◽  
Antonio Sánchez-Pozo
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Related Effect ◽  
Age Related ◽  
Dietary Nucleotides

scholarly journals Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary gland of lactating rats

1983 ◽  
Vol 212 (1) ◽  
pp. 149-153 ◽  
Author(s):  
M E Brosnan ◽  
R Farrell ◽  
H Wilansky ◽  
D H Williamson
Keyword(s):  
Mammary Gland ◽  
Nucleic Acid ◽  
Ornithine Decarboxylase ◽  
Acid Content ◽  
Marked Decrease ◽  
Mammary Glands ◽  
Nucleic Acid Content ◽  
Decarboxylase Activity ◽  
Ornithine Decarboxylase Activity ◽  
Polyamine Content

Starvation caused a marked increase in putrescine content in mammary gland of lactating rats, together with a marked decrease in activity of ornithine decarboxylase and appearance of measurable ornithine decarboxylase antizyme. 2. Refeeding for 5 h caused disappearance of free antizyme and ornithine decarboxylase activity returned to the value in fed animals. Putrescine concentration remained elevated. 3. There was no significant change in nucleic acid content of mammary gland from starved rats, but spermidine and spermine contents increased significantly. 4. Refeeding for 5 h returned the spermidine content of mammary glands to ‘fed’ values, and significantly decreased the content of spermine, although it did not reach control values. Thus changes in polyamine content of mammary gland in starved rats are clearly dissociated from changes in either RNA content or activities of polyamine-synthetic decarboxylases. 5. Starvation caused a fall in the content of spermidine in liver, with no change in spermine content. Refeeding for 5 h returned the spermidine content to ‘fed’ values.

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