Effect of Season on the Follicle Stimulating Hormone and Luteinizing Hormone Potency of Sheep Anterior Pituitary Glands

Nature ◽  
1960 ◽  
Vol 188 (4750) ◽  
pp. 585-586 ◽  
Author(s):  
J. S. M. HUTCHINSON ◽  
HAMISH ROBERTSON
Keyword(s):  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
Follicle Stimulating Hormone ◽  
Pituitary Glands ◽  
Stimulating Hormone

Some effects of level of feeding and body condition upon sperm production and gonadotropin concentrations in the ram

1982 ◽  
Vol 34 (3) ◽  
pp. 265-277 ◽  
Author(s):  
J. E. Alkass ◽  
M. J. Bryant ◽  
J. S. Walton
Keyword(s):  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
Reproductive Tract ◽  
Follicle Stimulating Hormone ◽  
Live Weight ◽  
Maintenance Requirement ◽  
Scrotal Circumference ◽  
Significant Difference ◽  
Pituitary Glands ◽  
Stimulating Hormone

ABSTRACT1. In two experiments. Scottish Blackface and Finnish Landrace ♂ × Dorset Horn ♀ rams were housed in light-proof buildings and subjected to a constant 10-h light: 14-h dark photoperiod 70 days before and during the experiment. In experiment 1, four rams from each breed type were given either 750kJ/MJ(L) or 1250 to 1500 kJ/MJ(H) of their maintenance requirement for 20 weeks. In experiment 2, four rams of each breed were given either 750kJ/MJ(T) or 2000 kJ/MJ(F) of their maintenance requirement for 20 weeks, and were then fed to maintain live weight for a further 10 weeks. Daily sperm output in the urine and scrotal circumference were measured in both experiments. Various characteristics of ejaculated semen were measured in experiment 1. Sequential blood samples were collected from the rams on both experiments and assayed for follicle stimulating hormone and luteinizing hormone. At the end of each experiment the rams were slaughtered, and their testes, epididymides and anterior pituitary glands recovered.2. In Experiment 1, the L feeding level depressed progressively the numbers of sperm voided in the urine, scrotal circumference, and the concentration and total numbers of sperm in ejaculated semen. The testes and epididymides of L rams were lighter (P<0·01) and fewer sperm were recovered from the reproductive tract than i n H rams (P<0·01). The pituitary glands were lighter (P<0·05) and contained less luteinizing hormone (P<0·01) but amounts of follicle stimulating hormone similar to the H rams. Plasma luteinizing hormone levels were lower in L than in H rams at 20 weeks (P<0·05).3. In experiment 2, the T rams showed a progressive depression of numbers of sperm in the urine and scrotal circumference, such that at 20 weeks differences between F and T treatments were statistically significant (P<0·05 and 001 respectively). Subsequently, some recovery occurred which, with a reduction in these measures on F treatments, led to there being no statistically significant difference at 30 weeks. There were no statistically significant differences between F and T treatments in testes and epididymes weights, total number of sperm in the tract, and anterior pituitary weight, follicle stimulating hormone and luteinizing hormone content.

Effect of Starvation on Pituitary and Serum Follicle-Stimulating Hormone and Luteinizing Hormone Following Ovariectomy in the Rat

1972 ◽  
Vol 50 (8) ◽  
pp. 768-773 ◽  
Author(s):  
E. A. Ibrahim ◽  
B. E. Howland
Keyword(s):  
Luteinizing Hormone ◽  
Pituitary Gland ◽  
Follicle Stimulating Hormone ◽  
Female Rats ◽  
Intact Female ◽  
Pituitary Glands ◽  
Ad Libitum ◽  
Stimulating Hormone

The concentration of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in serum and pituitary glands was studied in intact female rats and rats that were ovariectomized on day 0 of the experiment and then starved or fed for 2, 4, 7, or 9 days. Ovariectomy resulted in enhanced rates of synthesis and release of FSH and LH as indicated by the significant (P < 0.01) rises in the concentration of both hormones in the pituitary gland and serum.Starvation resulted in a decrease in body and pituitary weight. The concentration of FSH and LH in pituitary glands of starved rats was higher (P < 0.05) than that in fed rats on days 7 and 9. The concentration of FSH and LH in serum of starved rats was increased after ovariectomy but the levels on days 7 and 9 were lower than those of fed rats.These results suggest that the synthesis of FSH and LH was enhanced in both starved and fed rats following ovariectomy while the rate of release of both hormones was decreased at 7 and 9 days of starvation in comparison with rats fed ad libitum.

Inhibition of release of a novel pituitary polypeptide, 7B2, follicle-stimulating hormone, and luteinizing hormone from rat anterior pituitary cells in vitro by human β-inhibin

1986 ◽  
Vol 64 (9) ◽  
pp. 1259-1262 ◽  
Author(s):  
John S. D. Chan ◽  
Jie-Ying Deng ◽  
Anoop K. Brar ◽  
Nabil G. Seidah ◽  
Michel Chrétien
Keyword(s):  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
Polyclonal Antibodies ◽  
Follicle Stimulating Hormone ◽  
Feedback Mechanism ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Specific Radioimmunoassay ◽  
Stimulating Hormone

We have recently purified a novel pituitary polypeptide designated 7B2. By raising polyclonal antibodies to a synthetic 7B2 fragment in rabbits, we have developed a sensitive and specific radioimmunoassay for this novel polypeptide, and it has been used for the study of the release of immunoreactive 7B2 from rat anterior pituitary cells in vitro. In addition, immunocytochemical study shows that 7B2 is present in the gonadotropin cells of rat anterior pituitary. The aim of the present studies is to investigate the effect of human β-inhibin, testosterone, and combined testosterone plus human β-inhibin on the induced release of immunoreactive 7B2, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) in rat anterior pituitary cell culture in vitro. Our results show that both human β-inhibin and testosterone effectively suppress the stimulatory effect of luteinizing hormone-releasing hormone (LHRH) on immunoreactive 7B2, FSH, and LH release. The present data indicate that the regulation of secretion of 7B2 and pituitary gonadotropins may be under a similar type of feedback mechanism.

PURIFICATION OF FOLLICLE-STIMULATING HORMONE FROM HUMAN ANTERIOR PITUITARY GLANDS

1964 ◽  
Vol 42 (6) ◽  
pp. 841-849 ◽  
Author(s):  
W. H. McShan ◽  
B. B. Saxena ◽  
R. O. Creek
Keyword(s):  
Gel Electrophoresis ◽  
Anterior Pituitary ◽  
Follicle Stimulating Hormone ◽  
Starch Gel Electrophoresis ◽  
Thyrotropic Hormone ◽  
Pituitary Glands ◽  
Starch Gel ◽  
Gonadotropic Activity ◽  
Human Anterior Pituitary ◽  
Stimulating Hormone

The results of this study indicate that highly purified follicle-stimulating hormone (FSH) was prepared from human anterior pituitary glands by ammonium sulphate (AS) fractionation, zone electrophoresis, and starch gel electrophoresis. The activity of this preparation was approximately 14.7 times that of the sheep pituitary FSH standard. The fractions from zone and starch gel electrophoresis with which luteinizing hormone (LH) was associated also contained thyrotropic hormone (TSH). There was little decrease in the gonadotropic activity of human anterior pituitary glands recovered at different times up to 24 hours post-mortem.

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