An Effect of Tritiated Thymidine on the Incorporation of Thymidine into Chromosomal Deoxyribonucleic Acid

Nature ◽  
1960 ◽  
Vol 188 (4749) ◽  
pp. 511-512 ◽  
Author(s):  
MARGARIDA KRAUSE ◽  
W. PLAUT
Keyword(s):  
Deoxyribonucleic Acid ◽  
Tritiated Thymidine

INCORPORATION OF RADIOACTIVE PURINE AND PYRIMIDINE BASES AND OF THYMIDINE INTO THE DEOXYRIBONUCLEIC ACID OF SALMON MILTS

1964 ◽  
Vol 42 (1) ◽  
pp. 51-57 ◽  
Author(s):  
H. L. A. Tarr
Keyword(s):  
Orotic Acid ◽  
Deoxyribonucleic Acid ◽  
Tritiated Thymidine ◽  
Experimental Conditions ◽  
Live Fish ◽  
Pyrimidine Bases

C14-labeled adenine, guanine and cytosine, and tritiated thymidine were incorporated into the deoxyribonucleic acid of salmon milts, either by injection into the milts of live fish or into excised milts. The amount incorporated was very small. Under the experimental conditions radioactive nucleosides, deoxyuridine, adenosine 5′-mono- and tri-phosphates, orotic acid, uracil, ribose 1-phosphate, and ribose 5-phosphate were not incorporated. It is suggested that these results may be due to the comparative impermeability of the cells to the various compounds.

EFFECTS OF A GLUCOCORTICOID (DEXAMETHASONE) ON THE EOSINOPHILS OF THE RAT

1967 ◽  
Vol 37 (4) ◽  
pp. 463-469 ◽  
Author(s):  
EVELYN C. BLENKINSOPP ◽  
W. K. BLENKINSOPP
Keyword(s):  
Continuous Infusion ◽  
Deoxyribonucleic Acid ◽  
Tritiated Thymidine ◽  
Blood Eosinophil ◽  
Consequent Reduction ◽  
Reticulo Endothelial System ◽  
Eosinophil Counts

SUMMARY The effects of single and continuous administrations of a synthetic gluco-corticoid (dexamethasone) on eosinophil numbers and distribution have been studied. Absolute blood eosinophil counts were made, and the turnover of the eosinophils was examined by continuous infusion of tritiated thymidine to label the deoxyribonucleic acid of all newly formed cells. Dexamethasone equivalent to less than 200 μg. cortisone/100 g. rat/day produced disappearance of eosinophils from the blood and the normal output of glucocorticoids is therefore probably less than this. Single administrations of dexamethasone produced a blood eosinopenia within 2 hr. due to removal and destruction of eosinophils by the reticulo-endothelial system. Continuous dexamethasone administration reduced the number of proliferating eosinophil cells in the marrow, with a consequent reduction in the number of eosinophils in the tissues.

Duration of Availability of Tritiated Thymidine Following Intraperitoneal Injection

1968 ◽  
Vol 3 (1) ◽  
pp. 89-93
Author(s):  
W. K. BLENKINSOPP
Keyword(s):  
Cell Division ◽  
Intraperitoneal Injection ◽  
Deoxyribonucleic Acid ◽  
Direct Evidence ◽  
Tritiated Thymidine ◽  
Indirect Evidence ◽  
Acid Synthesis ◽  
Single Intraperitoneal Injection ◽  
Deoxyribonucleic Acid Synthesis

Much indirect evidence supports the assumption that tritiated thymidine does not label cells which enter the deoxyribonucleic acid synthesis phase (S) more than 1 h after injection. Direct evidence confirming this assumption was obtained by counting labelled epithelial nuclei in mice killed 1, 4 or 6 h after a single intraperitoneal injection of [3H]thymidine; colchicine was used to prevent the increase in number of labelled nuclei which would otherwise have occurred because of cell division. The proportion of cells labelled was the same at 1 h as at 4 or 6 h after injection of [3H]thymidine. Nuclei were regarded as labelled if they were overlaid by 4 grains or more; comparison of nuclear and background labelling indicated that nuclei overlaid by 3 grains or less represented background labelling.

scholarly journals Study upon The Tritiated Thymidine Incorporation into Deoxyribonucleic Acid of Tissue Cultured HeLa Cells

RADIOISOTOPES ◽  
1961 ◽  
Vol 10 (1) ◽  
pp. 135-139
Author(s):  
Toyozo SEKIGUCHI ◽  
Takako KANKURA ◽  
Hideo ETO ◽  
Tetuo IWAKURA
Keyword(s):  
Hela Cells ◽  
Deoxyribonucleic Acid ◽  
Thymidine Incorporation ◽  
Tritiated Thymidine

DEOXYRIBONUCLEIC ACID LABELLING AFTER TRITIATED THYMIDINE INJECTION

1966 ◽  
Vol 44 (3) ◽  
pp. 451-454 ◽  
Author(s):  
N. Brière ◽  
H. Isler
Keyword(s):  
Tissue Culture ◽  
Deoxyribonucleic Acid ◽  
Tritiated Thymidine ◽  
Time Intervals ◽  
Young Rats ◽  
Highly Sensitive

Young rats were injected with thymidine-3H and the level of radioactivity in their blood was measured by a highly sensitive radioautographic technique. Radioactivity (10−6 μcurie/ml) was still detected in serum 7 days after injection. To determine whether the blood of these rats contained deoxyribonucleic acid (DNA) precursors, the sera, taken at various time intervals after injection, were introduced into tissue culture chambers. By radioautography of these cultures, it was found that they contained cells with labelled nuclei, even when exposed to sera taken 30 hours after injection. The presence of nuclear precursors in these sera probably indicates a reutilization of DNA from other cells.

Automated analysis of antigen-stimulated lymphocytes.

1976 ◽  
Vol 24 (1) ◽  
pp. 373-377 ◽  
Author(s):  
M Cassidy ◽  
C Yee ◽  
J Costa
Keyword(s):  
Deoxyribonucleic Acid ◽  
Human Peripheral Blood ◽  
Tritiated Thymidine ◽  
Automated Analysis ◽  
Peripheral Blood Lymphocytes ◽  
Type I ◽  
Lymphocyte Response ◽  
Purified Protein Derivative ◽  
Blastogenic Response ◽  
Low Levels

Human peripheral blood lymphocytes were stimulated with phytohemagglutinin and tuberculin-purified protein derivative to determine if flow microfluorometric techniques had the sensitivity to detect high and low levels of blastogenic response. The deoxyribonucleic acid content of the lymphocytes was analyzed after culture. Blastogenic response was found to be measurable both sensitively and reproducibly. Tritiated thymidine incorporation measurements were also made. Preliminary experiments using inactivated Herpesvirion (type I) as a stimulant showed blastogenic response detectable by flow microfluorometric deoxyribonucleic acid measurements. Refinements of this technique may prove to be useful in the study of lymphocyte response to viral antigens in patient populations.

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