Growth by Cell Division in Insect Tissue Culture

Nature ◽  
1960 ◽  
Vol 187 (4743) ◽  
pp. 1072-1074 ◽  
Author(s):  
B. M. JONES ◽  
I. CUNNINGHAM
Keyword(s):  
Tissue Culture ◽  
Cell Division ◽  
Insect Tissue Culture

Growth by cell division in insect tissue culture

1961 ◽  
Vol 23 (2) ◽  
pp. 386-401 ◽  
Author(s):  
B.M. Jones ◽  
I. Cunningham
Keyword(s):  
Tissue Culture ◽  
Cell Division ◽  
Insect Tissue Culture

Tests by Tissue Culture Methods on the Nature of Immunity Transplanted Skin

1948 ◽  
Vol s3-89 (7) ◽  
pp. 239-252
Author(s):  
P. B. MEDAWAR
Keyword(s):  
Tissue Culture ◽  
Cell Division ◽  
Epidermal Cell ◽  
Cell Suspensions ◽  
Acquired Immunity ◽  
Culture Methods ◽  
Division Frequency

The transplantation of skin from one rabbit to another elicits a reaction that conforms in main outline with that of an actively acquired immunity. The experiments described in this paper were designed to test the hypothesis that the regression of such grafts is secured by the action of antibodies demonstrable in vitro. Skin from adult rabbits has therefore been cultivated in the presence of serum and growing mesenchymal tissues derived solely from rabbits heavily and specifically immunized against it. Immune sera and tissues are without effect on the survival, cell-division frequency and migratory activities of explanted skin, and agglutinins for epidermal cell suspensions are not demonstrable in immune sera. With certain stated qualifications, it has therefore been concluded that the occurrence of free antibodies is not a sufficient explanation of the regression of skin homografts in vivo.

scholarly journals Cation Metabolism in Relation to Cell Size in Synchronously Grown Tissue Culture Cell

1967 ◽  
Vol 50 (4) ◽  
pp. 917-932 ◽  
Author(s):  
Chan Jung ◽  
Aser Rothstein
Keyword(s):  
Tissue Culture ◽  
Cell Division ◽  
Exponential Function ◽  
Single Cells ◽  
Cell Number ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Content Increase ◽  
Cation Content ◽  
Unidirectional Fluxes

In randomly grown tissue culture cells (mouse leukemic lymphoblast, L5178Y) the number, volume, and Na+ and K+ content increase as an exponential function with a doubling time of 11.3 hr. In synchronously grown cells the volume increase of the population and of single cells follows the same exponential function as in randomly grown cells. In contrast, the cation content fluctuates during a single cell cycle. About 1½ hr after the cell division burst (at the beginning of the S period), a net loss of K+ occurs for a period of about 1 hr amounting to about 20% of the total K. Over the next 5 to 6 hr, the deficit in K+ is eliminated. The Na+ content shows a double fluctuation. It falls during the cell division burst, rises when the K+ content decreases, falls again when K+ content rises, and then increases again before the next cell division burst. The net fluxes of both Na+ and K+ are very small compared to the unidirectional fluxes (less than 5%), thus small changes in the balance of influx and efflux account for the changes in cation content during the growth cycle. Both unidirectional fluxes increase dramatically (by a factor of two) about 2 hr after the cell division burst, and then remain constant until after the next cell division. The pattern of electrolyte regulation during cell division does not follow a simple function such as cell number, cell surface, or cell volume, but must be related to specific internal events in the cell.

Citrus tissue culture: regulation of stylar abscission in excised pistils

1980 ◽  
Vol 58 (11) ◽  
pp. 1257-1261 ◽  
Author(s):  
John W. Einset ◽  
Anne Cheng ◽  
Hamid Elhag
Keyword(s):  
Tissue Culture ◽  
Cell Division ◽  
Dichlorophenoxyacetic Acid ◽  
Navel Orange ◽  
Test Medium ◽  
Fresh Weight ◽  
Valencia Orange ◽  
Washington Navel ◽  
Citrus Tissue Culture ◽  
2,4 Dichlorophenoxyacetic Acid

Lemon pistil explants were obtained by cutting just above the region of the hypogynous disc (A type explant) or at the base of the pistil (B type explant) and cultured on test medium containing Murashige and Skoog salts, 50 g sucrose/L, 100 mg myo-inositol/L, 5 mg thiamine–HCl/L, and 0.5 mg kinetin/L, plus or minus supplements. Under appropriate conditions an abscission zone formed and styles abscised after 6–8 days of culture; in the field stylar abscission occurred 12–15 days postanthesis. Abscission in A type explants was markedly inhibited by 9 μM 2,4-dichlorophenoxyacetic acid but was unaffected by indole-3-acetic, 1-naphthaleneacetic, gibberellic, abscisic, caffeic, or p-coumaric acids. The response to 2,4-dichlorophenoxyacetic acid was reduced in B type explants. In an atmosphere containing 35–200 ppm ethylene, cell division occurred in the zone of stylar abscission producing a proliferating callus, and the content of cellulase increased from 0.6 to 53.7 enzyme units/g fresh weight compared with fresh explants. Stylar abscission was inhibited by 2,4-dichlorophenoxyacetic acid in A type explants of Washington navel orange, Valencia orange, and mandarin pistils, but not of grapefruit pistils. B type explants of Washington navel orange and mandarin pistils were less responsive to 2,4-dichlorophenoxyacetic acid.

Insect Tissue Culture: Use of Blastokinetic Stage of Leafhopper Embryo

Science ◽  
1964 ◽  
Vol 144 (3625) ◽  
pp. 1465-1467 ◽  
Author(s):  
H. Hirumi ◽  
K. Maramorosch
Keyword(s):  
Tissue Culture ◽  
Insect Tissue Culture

Morphogenesis in Insect Tissue Culture

1982 ◽  
pp. 237-265 ◽  
Author(s):  
Herbert Oberlander ◽  
Dwight E. Lynn
Keyword(s):  
Tissue Culture ◽  
Insect Tissue Culture
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