Isolation of a Phagocytosis-inhibiting Substance from Culture Filtrates of an Encapsulated Staphylococcus aureus

Nature ◽  
1960 ◽  
Vol 186 (4718) ◽  
pp. 102-103 ◽  
Author(s):  
STEPHEN I. MORSE
Keyword(s):  
Staphylococcus Aureus ◽  
Culture Filtrates

STUDIES WITH STAPHYLOCOCCAL TOXINS: VI. SOME PROPERTIES OF A NON-HEMOLYTIC DERMONECROTIC TOXIN PRODUCED BY SOME STRAINS OF STAPHYLOCOCCUS AUREUS

1960 ◽  
Vol 6 (2) ◽  
pp. 195-201 ◽  
Author(s):  
J. Robinson ◽  
F. S. Thatcher ◽  
Jeannine Montford
Keyword(s):  
Staphylococcus Aureus ◽  
Proteolytic Activity ◽  
Carboxymethyl Cellulose ◽  
Dermonecrotic Toxin ◽  
Culture Filtrates ◽  
Zone Electrophoresis ◽  
Chromatographic Procedure ◽  
Selective Adsorbent

A non-hemolytic, dermonecrotic preparation with proteolytic activity was isolated from culture filtrates of some strains of Staphylococcus aureus using a chromatographic procedure with carboxymethyl-cellulose as a selective adsorbent. The preparation was resolved into components which were independently proteolytic and dermonecrotic using a zone electrophoresis procedure.The non-hemolytic dermonecrotic toxin possessed properties which characterize it as a protein. No relationship could be found between pathogenicity of the staphylococcus and its capacity to produce the toxin.The presence in some culture filtrates of the non-hemolytic, dermonecrotic toxin has been considered as a possible explanation for the lack of agreement noted by several authors between alpha hemolytic and dermonecrotic titers.

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

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