Trichloracetic Acid-Insoluble Phosphate Compounds in Cartilage

R. G. WHITEHEAD; S. M. WEIDMANN

doi:10.1038/183876a0

THE BIOCHEMISTRY OF THE NITRIFYING ORGANISMS: PART 7. THE PHOSPHATE COMPOUNDS OF NITROBACTER AND THE UPTAKE OF ORTHOPHOSPHATE BY THE ORGANISM

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o60-161 ◽

1960 ◽

Vol 38 (11) ◽

pp. 1295-1300

Author(s):

W. D. Butt ◽

H. Lees

Keyword(s):

Slow Rate ◽

Total Cell ◽

Nitrite Oxidation ◽

Trichloracetic Acid ◽

Alkali Extract ◽

Labile Component ◽

Autotrophic Bacterium ◽

Acid Labile ◽

Labile Compound ◽

Phosphate Compounds

The phosphate compounds present in the cells of the chemosynthetic autotrophic bacterium Nitrobacter were fractionated by means of successive extractions with cold 5% trichloracetic acid, ethanol, ethanol:ether, and dilute alkali. It was found that about 50% of the total cell phosphate was soluble in dilute alkali and, of this, 76% was acid-labile. Treatment of the alkali extract with Norit reduced the content of non-labile phosphate without affecting the acid-labile component; this labile compound was identified as a polyphosphate. By the use of 32P-labelled orthophosphate, it was shown that the cells absorbed orthophosphate from the medium at a slow rate during nitrite oxidation.

Download Full-text

THE BIOCHEMISTRY OF THE NITRIFYING ORGANISMS: PART 7. THE PHOSPHATE COMPOUNDS OF NITROBACTER AND THE UPTAKE OF ORTHOPHOSPHATE BY THE ORGANISM

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y60-161 ◽

1960 ◽

Vol 38 (1) ◽

pp. 1295-1300 ◽

Cited By ~ 1

Author(s):

W. D. Butt ◽

H. Lees

Keyword(s):

Slow Rate ◽

Total Cell ◽

Nitrite Oxidation ◽

Trichloracetic Acid ◽

Alkali Extract ◽

Labile Component ◽

Autotrophic Bacterium ◽

Acid Labile ◽

Labile Compound ◽

Phosphate Compounds

The phosphate compounds present in the cells of the chemosynthetic autotrophic bacterium Nitrobacter were fractionated by means of successive extractions with cold 5% trichloracetic acid, ethanol, ethanol:ether, and dilute alkali. It was found that about 50% of the total cell phosphate was soluble in dilute alkali and, of this, 76% was acid-labile. Treatment of the alkali extract with Norit reduced the content of non-labile phosphate without affecting the acid-labile component; this labile compound was identified as a polyphosphate. By the use of 32P-labelled orthophosphate, it was shown that the cells absorbed orthophosphate from the medium at a slow rate during nitrite oxidation.

Download Full-text