Splitting of the Electrophoretic Pattern of Serum Albumin during Short-term Action of Pepsin at Room Temperature

Nature ◽  
1959 ◽  
Vol 183 (4655) ◽  
pp. 190-191 ◽  
Author(s):  
E. ANNAU
2021 ◽  
pp. 108201322199884
Author(s):  
Rami Akkad ◽  
Ereddad Kharraz ◽  
Jay Han ◽  
James D House ◽  
Jonathan M Curtis

The odour emitted from the high-tannin fab bean flour ( Vicia faba var. minor), was characterized by headspace solid-phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC–MS). The relative odour activity value (ROAV) was used to monitor the changes in key volatile compounds in the flour during short-term storage at different temperature conditions. The key flavour compounds of freshly milled flour included hexanal, octanal, nonanal, decanal, 3-methylbutanal, phenyl acetaldehyde, (E)-2-nonenal, 1-hexanol, phenyl ethyl alcohol, 1-octen-3-ol, β-linalool, acetic acid, octanoic acid, and 3-methylbutyric acid; these are oxidative degradation products of unsaturated fatty acids and amino acids. Despite the low lipid content of faba beans, the abundances of aldehydes arising during room temperature storage greatly contributed to the flavour of the flour due to their very low odour thresholds. Two of the key volatiles responsible for beany flavour in flour (hexanal, nonanal) increased greatly after 2 weeks of storage at room temperature or under refrigerated conditions. These volatile oxidation products may arise as a result of enzymatic activity on unsaturated fatty acids, and was seen to be arrested by freezing the flour.


Author(s):  
Kamal Pandey ◽  
Bhaskar Basu ◽  
Sandipan Karmakar

“Smart cities” start with “Smart Buildings” that improve the quality of urban services while ensuring sustainability. The current scenario in India reveals that the corporate and residential building structures are incorporating various self-sustainable techniques. Out of the multiple factors governing the comfort of smart buildings, indoor room temperature is an important one, since it drives the need of cooling or heating through controlling systems. Around one-third of total energy consumption of commercial buildings in India is attributed to Heating, Ventilation and Air Conditioning (HVAC) systems. Accurate prediction of indoor room temperature helps in creating an efficient equilibrium between energy consumption and comfort level of the building, thus providing opportunities for efficient decision making for energy optimization. Considering Indian climatic and geographical conditions, this paper proposes an efficient decision making approach using Bayesian Dynamic Models (BDM) for short-term indoor room temperature forecasting of a corporate building structure. The results obtained from Bayesian Dynamic linear model, using Expectation Maximization (EM) algorithm, have been compared to standard Auto Regressive Integrated Moving Average (ARIMA) model, and have been found to be more accurate. Forecasting of indoor room temperature is a highly nonlinear phenomenon, so to further improve the accuracy of the linear models, a hybrid modeling approach has been proposed. The inclusion of state-of-the-art nonlinear models such as Artificial Neural Networks (ANNs) and Support Vector Regression (SVR) improves the forecasting accuracy of the linear models significantly. Results show that the hybrid model obtained using BDM and ANN is the best fit model.


2017 ◽  
Vol 13 (10) ◽  
pp. 5905-5913
Author(s):  
S. Saravanan ◽  
P.Senthil Kumar ◽  
T. Palanisamy ◽  
M. Ravichandran ◽  
V. Anandakrishnan ◽  
...  

AA6063-TiC composites have several weight percentages up to 9 wt. % were fabricated by using stir casting route method. The effects of the weight percentage of TiC particles on the microstructures and corrosion behavior of AA6063-TiC composites were studied. The results revealed that the AA6063-TiC composites exhibited higher density than the AA6063 matrix. The accelerated corrosion tests of AA6063-TiC composites in 3.5 wt. % NaCl aqueous solution at room temperature, the AA6063-TiC composites have better corrosion resistance than the AA6063 matrix. Increasing the weight percentage of the TiC particles to reduces the corrosion rate of the AA6063-TiC composites. In this process corrosion rate of 0.4402 mm/year for AA6063 matrix, 0.3891 mm/year for 3 wt. % , 0.3568 mm/year for 6 wt. % and 0.3062 mm/year for 9 wt. % of TiC particles respectively. The poor corrosion resistance of the composites can be attributed to the galvanic effects between the AA6063 matrix and TiC reinforcement.


2017 ◽  
Vol 6 (36) ◽  
pp. 2957-2962
Author(s):  
Reeta James ◽  
Jog Antony ◽  
Sreelakshmi Sreedhar ◽  
Rohit Mathew ◽  
Adarsh Surendran

Metabolites ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 515
Author(s):  
Daniela Duarte ◽  
Beatriz Castro ◽  
Joana Leonor Pereira ◽  
Joana Faria Marques ◽  
Ana Luísa Costa ◽  
...  

Maintaining a salivary metabolic profile upon sample collection and preparation is determinant in metabolomics. Nuclear magnetic resonance (NMR) spectroscopy was used to identify metabolite changes during short-term storage, at room temperature (RT)/4 °C/−20 °C, and after sample preparation, at RT/4 °C (mimicking typical clinical/laboratory settings). Interestingly, significant metabolic inter-individual and inter-day variability were noted, probably determining sample stability to some extent. After collection, no changes were noted at −20 °C (at least for 4 weeks). RT storage induced decreases in methylated macromolecules (6 h); lactate (8 h); alanine (12 h); galactose, hypoxanthine, pyruvate (24 h); sarcosine, betaine, choline, N-acetyl-glycoproteins (48 h), while acetate increased (48 h). Less, but different, changes were observed at 4 °C, suggesting different oral and microbial status at different temperatures (with a possible contribution from inter-individual and inter-day variability), and identifying galactose, hypoxanthine, and possibly, choline esters, as potential general stability indicators. After preparation, addition of NaN3 did not impact significantly on saliva stabilization, neither at RT nor at 4 °C, although its absence was accompanied by slight increases in fucose (6.5 h) and proline (8 h) at RT, and in xylose (24 h) at 4 °C. The putative metabolic origins of the above variations are discussed, with basis on the salivary microbiome. In summary, after collection, saliva can be stored at RT/4 °C for up to 6 h and at −20 °C for at least 4 weeks. Upon preparation for NMR analysis, samples are highly stable at 25 °C up to 8 h and at 4 °C up to 48 h, with NaN3 addition preventing possible early changes in fucose, proline (6–8 h), and xylose (24 h) levels.


2000 ◽  
Vol 20 (6) ◽  
pp. 703-707 ◽  
Author(s):  
Francisco Caravaca ◽  
Manuel Arrobas ◽  
Carmen Dominguez

Background Hypoalbuminemia is common in peritoneal dialysis (PD) patients; but the reduction in serum albumin levels (SAlb) that should be expected in stable PD patients is less clear. Objectives To determine prospectively, in a group of stable PD patients without comorbid conditions, the changes in SAlb concentration and in the concentrations of the other serum protein fractions. To investigate the best determinants of a significant decrease in SAlb levels. Design Prospective observational study. Methods Seventeen PD patients in stable clinical condition, with no signs of systemic inflammatory response, were included in the study. SAlb and the electrophoretic pattern of serum proteins were determined immediately before PD start, and after 6, 9, 12, 15, 18, 21, and 24 months on PD. In each study period, clinical characteristics, adequacy parameters, protein catabolic rate (PNPNA: protein equivalent of non protein nitrogen appearance), and protein losses were determined. Patients were divided into two subgroups according to whether SAlb decreased less than 10%, or 10% or more, from baseline values after 24 months on PD. The main differences between the subgroups were investigated. Results Mean SAlb did not decrease significantly after 24 months on PD (from baseline 3.99 ± 0.46 g/dL to 3.80 ± 0.54 g/dL), though percentage SAlb values did (58.36% ± 5.58% vs 55.15% ± 5.42%, p < 0.01). A weak increase in α2-globulin was observed after 18 months on PD (from 10.62% ± 2.53% to 12.96% ± 2.51%, p = 0.001). α1-Globulin showed a sustained increase from a mean baseline value of 3.51% ± 1.09% to 6.83% ± 2.13% after 24 months ( p < 0.0001). Seven patients had a reduction in SAlb greater than 10% after 24 months on PD. Kt/V urea and residual renal function tended to be lower in patients whose SAlb decreased. Mean PNPNA was significantly lower in patients who had a reduction in SAlb (0.76 ± 0.12 g/kg/day vs 0.96 ± 0.12 g/kg/day, p < 0.0001). However, total protein loss was even greater in patients who had no SAlb reduction. Conclusions After 24 months on PD, a mean reduction in SAlb of 10% – 15% from baseline values should be expected only in those stable patients whose PNPNA is low.


Author(s):  
Lewen Bi ◽  
Lanzhu Zhang

Bolted flange joints are widely used in petroleum, chemical, nuclear and power industries, etc. With more and more devices are used at high temperature, the performance of flange connections becomes more complex, especially with creep of different components in flange connection. At elevated temperature, with the loss of bolt force and gasket force due to creep, the joints are prone to leak. Based on this, this paper analyzed the relaxation of bolt force at elevated temperature due to creep of bolt, flange and gasket separately and simultaneously. Besides, the influence of different initial installation stress of bolts was also studied. The results showed bolted flange joints relaxed due to gasket creep during early short term service. However, contribution of bolt and flange creep became more and more significant with the extension of time. With considering the creep of bolt, flange and gasket simultaneously, 50% to 60% of the bolt material yield strength at room temperature was recommended as the bolt initial installation stress for the joint case studied in this paper.


Minerals ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 172
Author(s):  
Mamert Mbonimpa ◽  
Parrein Kwizera ◽  
Tikou Belem

When cemented paste backfill (CPB) is used to fill underground stopes opened in permafrost, depending on the distance from the permafrost wall, the curing temperature within the CPB matrix decreases progressively over time until equilibrium with the permafrost is reached (after several years). In this study, the influence of declining curing temperature (above freezing temperature) on the evolution of the unconfined compressive strength (UCS) of CPB over 28 days’ curing is investigated. CPB mixtures were prepared with a high early (HE) cement and a blend of 80% slag and 20% General Use cement (S-GU) at 5% and 3% contents and cured at room temperature in a humidity chamber and under decreasing temperatures in a temperature-controlled chamber. Results indicate that UCS is higher for CPB cured at room temperature than under declining temperatures. UCS increases progressively from the stope wall toward the inside of the CPB mass. Under declines in curing temperature, HE cement provides better short-term compressive strength than does S-GU binder. In addition, the gradual decline in temperature does not appear to affect the fact that the higher the binder proportion, the greater the strength development. Therefore, UCS is higher for samples prepared with 5% than 3% HE cement. Findings are discussed in terms of practical applications.


Sign in / Sign up

Export Citation Format

Share Document