Serological Detection of Fermentation Wastes

Nature ◽  
1958 ◽  
Vol 182 (4650) ◽  
pp. 1680-1681
Author(s):  
ROBERT L. BUNCH ◽  
EDWIN F. BARTH
1981 ◽  
Vol 256 (19) ◽  
pp. 9767-9769
Author(s):  
C.V. Mura ◽  
B.D. Stollar

2021 ◽  
Vol 10 (12) ◽  
pp. 2718
Author(s):  
Omid Madadi-Sanjani ◽  
Gunnar Bohlen ◽  
Fabian Wehrmann ◽  
Julia Andruszkow ◽  
Karim Khelif ◽  
...  

In biliary atresia (BA), apoptosis is part of the pathomechanism, which results in progressive liver fibrosis. There is increasing evidence suggesting that apoptotic liver injury can be non-invasively detected by measuring the caspase activity in the serum. The purpose of this study was to investigate whether serological detection of caspase activation mirrors apoptotic liver injury in the infective murine BA-model and represents a suitable biomarker for BA in humans. Analysis showed increased caspase-3 activity and apoptosis in the livers of cholestatic BALB/c mice, which correlated significantly with caspase activation in the serum. We then investigated caspase activation and apoptosis in liver tissues and sera from 26 BA patients, 23 age-matched healthy and 11 cholestatic newborns, due to other hepatopathies. Compared to healthy individuals, increased caspase activation in the liver samples of BA patients was present. Moreover, caspase-3 activity was significantly higher in sera from BA infants compared to patients with other cholestatic diseases (sensitivity 85%, specificity 91%). In conclusion, caspase activation and hepatocyte apoptosis play an important role in experimental and human BA. We demonstrated that serological detection of caspase activation represents a reliable non-invasive biomarker for monitoring disease activity in neonatal cholestatic liver diseases including BA.


2021 ◽  
Vol 23 ◽  
pp. 100539
Author(s):  
Ana Beatriz Vieira Sacchi ◽  
Marcos Rogério André ◽  
Ana Cláudia Calchi ◽  
Mariele de Santi ◽  
Andresa Guimarães ◽  
...  

2012 ◽  
Vol 107 (8) ◽  
pp. 1073-1075 ◽  
Author(s):  
Vanessa Melandri ◽  
Anthony Érico Guimarães ◽  
Nicholas Komar ◽  
Maurício L Nogueira ◽  
Adriano Mondini ◽  
...  

1994 ◽  
Vol 9 (2) ◽  
pp. 63-69 ◽  
Author(s):  
R. Moll

Cytokeratins, which comprise a multigene family of 20 related polypeptides (CKs 1–20), are constituents of the intermediate filaments of epithelial cells, in which they are expressed in various combinations depending on the epithelial type and the degree of differentiation. Of these, CK 19 (400 amino acids; 44.1 kilodaltons) is an example of a widely distributed CK, being expressed in various epithelia, including many simple epithelia. In contrast, the recently identified CK 20 (424 amino acids; 48.6 kilodaltons) is essentially confined to gastrointestinal epithelia, the urothelium and Merkel cells. The differential expression of individual CKs in various types of carcinomas makes them useful markers for histopathological carcinoma subtyping, providing relevant information concerning the differentiation and origin of carcinomas, especially when tumors first present as metastases. The CKs that are of particular value for differential diagnosis include CK 20, as it is mainly expressed in carcinomas derived from CK 20-positive epithelia; it is also found in bile-tract, pancreatic and mucinous ovarian adenocarcinomas, being absent in most other carcinomas. In certain carcinoma types, the changes in the expression of individual CKs that may occur during tumor progression could be of prognostic relevance. It remains to be established whether the serological detection of fragments of not only widely distributed but also more restrictedly expressed CKs may provide useful serological tumor markers in the future.


2015 ◽  
Vol 114 (S1) ◽  
pp. 145-154 ◽  
Author(s):  
Manuela Schnyder ◽  
Roland Schaper ◽  
Zoltán Lukács ◽  
Sándor Hornok ◽  
Róbert Farkas

2019 ◽  
Vol 34 ◽  
pp. 10-13 ◽  
Author(s):  
Abdul Rahman Alashraf ◽  
Seng Fong Lau ◽  
Kuan Hua Khor ◽  
Siti Khairani-Bejo ◽  
Abdul Rani Bahaman ◽  
...  

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