scholarly journals Labelling of Cells in Thoracic Duct Lymph of the Guinea Pig after Tritiated Thymidine

Nature ◽  
1958 ◽  
Vol 182 (4649) ◽  
pp. 1608-1608 ◽  
Author(s):  
J. M. YOFFEY ◽  
N. B. EVERETT ◽  
W. O. REINHARDT
Keyword(s):  
Guinea Pig ◽  
Thoracic Duct ◽  
Tritiated Thymidine ◽  
Thoracic Duct Lymph ◽  
Duct Lymph

scholarly journals The Appearance of Labeled Cells in the Thoracic Duct Lymph of the Guinea Pig after the Administration of Tritiated Thymidine

Blood ◽  
1960 ◽  
Vol 15 (1) ◽  
pp. 82-94 ◽  
Author(s):  
N. B. EVERETT ◽  
W. O. REINHARDT ◽  
J. M. YOFFEY
Keyword(s):  
Single Dose ◽  
Guinea Pig ◽  
Intravenous Injection ◽  
Thoracic Duct ◽  
Guinea Pigs ◽  
Tritiated Thymidine ◽  
Thoracic Duct Lymph ◽  
Duct Lymph ◽  
Cell Series ◽  
Sequential Appearance

Abstract Tritium-labeled thymidine was given by either intraperitoneal or intravenous injection to 13 male guinea pigs of approximately 400 Gm. weight. At times varying from 1 hour to 30 days after the administration of thymidine, thoracic duct lymph was obtained and examined for the presence of labeled cells. After a single dose of thymidine, a steady stream of labeled lymphocytes, ranging from 2 to 7 per cent of the total cells, enters the blood over the period studied. The intensity of the labeling appears to diminish gradually. Labeled large and medium lymphocytes were found in the lymph during the first hour. Labeled small lymphocytes began to appear in the fourth hour, in small numbers, and thereafter increased, whereas the proportion of labeled large and medium lymphocytes steadily diminished. This sequential appearance of large, medium and small lymphocytes is interpreted as indicating the pattern of development of the cell series. The labeled small lymphocytes appearing in the lymph are considered to be newly formed from precursor cells located in the various lymphatic tissues.

scholarly journals Characterization of nonlymphoid cells derived from rat peripheral lymph

1983 ◽  
Vol 157 (6) ◽  
pp. 1758-1779 ◽  
Author(s):  
CW Pugh ◽  
GG MacPherson ◽  
HW Steer
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Thoracic Duct ◽  
Tritiated Thymidine ◽  
Thoracic Duct Lymph ◽  
Nonspecific Esterase ◽  
Cytoplasmic Inclusions ◽  
Peripheral Lymph ◽  
Duct Lymph

Mesenteric lymphadenectomy in rats is followed by union of peripheral and central lymphatics, allowing the collection of intestine-derived peripheral lymph cells via the thoracic duct for several days. These cells include a proportion of nonlymphoid cells (NLC) that show irregular and heterogeneous surface morphology including long pseudopodia and veils. They stain variably for nonspecific esterase and acid phosphatase and are ATPase-positive. Their nuclei are irregular and some contain cytoplasmic inclusions, some of which show peroxidase activity and/or contain DNA. NLC have a range of densitites generally lower than that of lymphocytes. Freshly collected NLC express the leukocyte-common antigen (defined by monoclonal antibody MRC Ox 1) and Ia antigens (I-A and I-E subregion products defined by monoclonal antibodies) but they show a relative lack of other surface markers normally found on rat B or T lymphocytes (W3/13, W3/25, MRC Ox 12 (sIg), MRC Ox 19) or rat macrophages (FcR, C'R, mannose R, W3/25). In general NLC are only weakly adherent to glass or plastic. Although a subpopulation of NLC appear to have had a phagocytic past, freshly collected NLC fail to phagocytose a variety of test particles in vitro. NLC also appear incapable of pinocytosis in vitro. This heterogeneity may represent distinct subpopulations of NLC or different stages in the development of a single cell lineage. Direct cannulation of mesenteric lacteals shows that the majority of NLC are derived from the small intestine and their precursors appear to be present both in lamina propria and Peyer's patches. Kinetic studies, following irradiation or intravenous tritiated thymidine, show that the majority of NLC turn over rapidly in the intestine with a modal time of 3-5 d. Studies with bone marrow chimeras show that they are derived from a rapidly dividing precursor present in normal bone marrow. NLC occur at very low frequencies in normal thoracic duct lymph at all times following cannulation. The evidence presented suggests that NLC closely resemble mouse lymphoid dendritic cells. This conclusion is supported by evidence already obtained showing that NLC are potent stimulators of the semi-allogeneic rat primary mixed leukocyte reaction. In addition to the ceils resembling dendritic cells rare monocytoid cells are found in thoracic duct lymph of lymphadenectomized specific pathogen-free rats. The proportion of these cells increases greatly when the animals are conventionally housed. It seems probable that the physiological function of NLC is to act as accessory cells in the lymph nodes to which they normally drain. Methods for enriching NLC and thus facilitating analysis of their functions are discussed.

scholarly journals THE ORIGIN AND TURNOVER OF MONONUCLEAR CELLS IN PERITONEAL EXUDATES IN RATS

1966 ◽  
Vol 124 (2) ◽  
pp. 241-254 ◽  
Author(s):  
Alvin Volkman
Keyword(s):  
Bone Marrow ◽  
Experimental Evidence ◽  
Thoracic Duct ◽  
Mononuclear Cells ◽  
Tritiated Thymidine ◽  
Thoracic Duct Lymph ◽  
Duct Lymph ◽  
Kinetics Of

Tritiated thymidine-labeling data in individual and parabiotic rats showed that macrophages in peritoneal exudates were derived from cells in the blood which were the progeny of rapidly and continuously proliferating precursors. The characteristics of this population identify them with free macrophages studied in other sites; similarly, they can be obtained from transfused bone marrow. Cells in the exudates which were morphologically indistinguishable from small lymphocytes were also found to have the labeling features of a rapidly proliferating population in contrast with the known kinetics of the majority of small lymphocytes in blood and thoracic duct lymph. However, experimental evidence indicated that the lymphocytelike exudate cells had emigrated from the blood and that bone marrow was a source of their precursors. These findings support the concept of the heterogeneity of lymphocytes. The possible relationships among the mononuclear cells is discussed.

Thoracic Duct Lymph and Lymphocytes in the Guinea Pig

1956 ◽  
Vol 187 (3) ◽  
pp. 493-500 ◽  
Author(s):  
W. O. Reinhardt ◽  
J. M. Yoffey
Keyword(s):  
Guinea Pig ◽  
Thoracic Duct ◽  
Marked Decrease ◽  
Lymph Flow ◽  
Thoracic Duct Lymph ◽  
Experimental Conditions ◽  
Duct Lymph ◽  
Order Of Magnitude ◽  
Adequate Stimulus ◽  
The Common

The guinea pig is a suitable animal for the collection of thoracic and cervical duct lymph. Values have been ascertained for lymph flow and lymphocyte output in the anesthetized male guinea pig under various experimental conditions. Evidence is presented which suggests the existence of a diurnal rhythm in level of thoracic duct output of lymphocytes. Effects of fasting and of hypoxia have been observed as examples of stimuli operating within a physiological range. Effects of interventions such as evisceration, biliary obstruction and fistulation, and treatment with adrenaline have also been observed. As compared with normal values, hypoxia was found to be an adequate stimulus to produce an increase in lymph flow and lymphocyte output. Fasting and treatment with adrenaline produced decreased lymphocyte output by the thoracic duct. Evidence provided by study after evisceration suggests that about 95% of the cells in the thoracic duct are derived from the lymphatic tissues associated with the gastroenteric tract and related tissues, residual thoracic duct lymph flow after evisceration being of the same order of magnitude as for the cervical lymph ducts. Obstruction of the common bile duct increased thoracic duct lymph flow as compared with the decrease produced by biliary fistulation. Removal of the thymus was associated with a marked decrease in the output of thoracic duct lymphocytes in both normal and splenectomized guinea pigs. Splenectomy was not associated with such a decrease in thoracic duct lymphocyte output.

scholarly journals Studies of Lymphocyte Kinetics in Man

Blood ◽  
1967 ◽  
Vol 29 (1) ◽  
pp. 22-28 ◽  
Author(s):  
SEYMOUR PERRY ◽  
GEORGE L. IRVIN ◽  
JACQUELINE WHANG
Keyword(s):  
Thoracic Duct ◽  
Peripheral Blood ◽  
Specific Activity ◽  
Tritiated Thymidine ◽  
Animal Experiments ◽  
Thoracic Duct Lymph ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Duct Lymph ◽  
Male Patients

Abstract Studies of certain aspects of lymphocyte kinetics were performed in nine patients with malignancies but who were hematologically normal. Following the administration of tritiated thymidine, well-labeled large lymphocytes appeared very promptly in thoracic duct lymph along with some lightly labeled small lymphocytes. Specific activity was higher in the thoracic duct lymph lymphocytes as compared to the peripheral blood leukocytes for at least the first 50 hours. When male patients were transfused with thoracic duct lymphocyte obtained from female donors, lymphocytes with a female karyotype were observed as early as 10 hours in the thoracic duct lymph and as early as one hour in the peripheral blood. The evidence presented in these studies confirms data previously obtained only in animal experiments and indicates that homologous lymphocytes may circulate as long as 9 days in appropriate recipients.

Measurement of Pancreatic Enzymes in Human Thoracic Duct Lymph

1960 ◽  
Vol 38 (6) ◽  
pp. 954-956 ◽  
Author(s):  
Allan E. Dumont ◽  
John H. Mulholland
Keyword(s):  
Thoracic Duct ◽  
Pancreatic Enzymes ◽  
Thoracic Duct Lymph ◽  
Duct Lymph

Thoracic duct lymph. Relative contribution from splanchnic and muscle tissue

Diabetes ◽  
1993 ◽  
Vol 42 (5) ◽  
pp. 720-731 ◽  
Author(s):  
G. M. Steil ◽  
M. A. Meador ◽  
R. N. Bergman
Keyword(s):  
Muscle Tissue ◽  
Thoracic Duct ◽  
Thoracic Duct Lymph ◽  
Relative Contribution ◽  
Duct Lymph

Dynamics of glucose production and uptake are more closely related to insulin in hindlimb lymph than in thoracic duct lymph

Diabetes ◽  
1994 ◽  
Vol 43 (2) ◽  
pp. 180-190 ◽  
Author(s):  
R. A. Poulin ◽  
G. M. Steil ◽  
D. M. Moore ◽  
M. Ader ◽  
R. N. Bergman
Keyword(s):  
Thoracic Duct ◽  
Glucose Production ◽  
Thoracic Duct Lymph ◽  
Duct Lymph

Lymphatic pump treatment mobilizes leukocytes from the gut associated lymphoid tissue into thoracic duct lymph

2008 ◽  
Vol 11 (4) ◽  
pp. 149 ◽  
Author(s):  
Artur Schander ◽  
Melissa K. Bearden ◽  
Jamie B. Huff ◽  
Arthur Williams ◽  
Scott T. Stoll ◽  
...  
Keyword(s):  
Thoracic Duct ◽  
Lymphoid Tissue ◽  
Thoracic Duct Lymph ◽  
Lymphatic Pump Treatment ◽  
Duct Lymph
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